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Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain

Nature Structural Biology volume 2, pages 898–905 (1995)Cite this article

Abstract

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two α-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl Sγ hydrogen bonds stablizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

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Author notes

  1. Kevin H. Gardner

    Present address: Department of Medical Genetics, Medical Sciences Building, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

  2. Stephen F. Anderson

    Present address: Department of Pathology, Brigham and Women's Hospital/Harvard Medical School, Boston, Massachusetts, 02115, USA

Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520-8114, USA

    Joseph E. Coleman

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  3. Joseph E. Coleman
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Gardner, K., Anderson, S. & Coleman, J. Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain. Nat Struct Mol Biol 2, 898–905 (1995). https://doi.org/10.1038/nsb1095-898

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