Abstract
Telomerases contain an essential RNA subunit (TER), as well as an essential protein reverse transcriptase subunit (TERT). The RNA subunit includes a short template region that is copied into telomeric DNA, but otherwise it is large and divergent. However, phylogenetic studies have revealed a conserved core secondary structure for TER. Much of the divergence can be accounted for by the acquisition of different types of RNA domains that function in RNA stabilization. Some of the nontemplate portions of TER, which include regions in the conserved core, are important for aspects of telomerase enzymatic activity independent of their role in telomerase assembly. Mutational studies indicate that telomerase enzyme function results from a collaboration of both protein and RNA functional groups contributed by TERT and TER.
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References
Greider, C.W. & Blackburn, E.H. Nature 337, 331–337 (1989).
Yu, G.-L., Bradley, J.D., Attardi, L.D. & Blackburn, E.H. Nature 344, 126–132 (1990).
Nakamura, T.M. & Cech, T.R. Cell 92, 587–590 (1998).
Aigner, S. et al. EMBO J. in the press (2000).
Nugent, C.I. & Lundblad, V. Genes Dev. 12, 1073–1085 (1998).
Mitchell, J.R., Wood, E. & Collins, K. Nature 402, 551–555 (1999).
Harrington, L. et al. Genes Dev. 11, 3109–3115 (1997).
Le, S., Sternglanz, R. & Greider, C.W. Mol. Biol. Cell 11, 999–1010 (2000).
Holt, S.E. et al. Genes Dev. 13, 817–826 (1999).
Weinrich, S.L. et al. Nature Genet. 17, 498–502 (1997).
Ritchie, K.B., Mallory, J.C. & Petes, T.D. Mol. Cell. Biol. 19, 6065–6075 (1999).
Naito, T., Matsuura, A. & Ishikawa, F. Nature Genet. 20, 203–206 (1998).
Greider, C.W. & Blackburn, E.H. Cell 51, 887–898 (1987).
Romero, D.P. & Blackburn, E.H. Cell 67, 343–353 (1991).
McCormick-Graham, M. & Romero, D.P. Nucleic Acids Res. 23, 1091–1097 (1995).
Lingner, J., Hendrick, L.L. & Cech, T.R. Genes Dev. 8, 1984–1998 (1994).
Chen, J.L., Blasco, M.A. & Greider, C.W. Cell 100, 503–514 (2000).
Gilley, D. & Blackburn, E.H. Proc. Natl. Acad. Sci. USA 96, 6621–6625 (1999).
Seto, A.G., Zaug, A.J., Sobel, S.G., Wolin, S.L. & Cech, T.R. Nature 401, 177–180 (1999).
Mitchell, J.R. & Collins, K. Mol. Cell in the press (2000).
Licht, J.D. & Collins, K. Genes Dev. 13, 1116–1125 (1999).
Autexier, C. & Triki, I. Nucleic Acids Res. 27, 2227–2234 (1999).
Roy, J., Fulton, T.B. & Blackburn, E.H. Genes Dev. 12, 3286–3300 (1998).
Beattie, T.L., Zhou, W., Robinson, M.O. & Harrington, L. Mol. Biol. Cell in the press (2000).
Shippen-Lentz, D. & Blackburn, E.H. Science 247, 546–552 (1990).
Wang, H., Gilley, D. & Blackburn, E.H. EMBO J. 17, 1152–1160 (1998).
Bryan, T.M., Goodrich, K.J. & Cech, T.R. Mol. Cell in the press (2000).
Collins, K. & Greider, C.W. Genes Dev. 7, 1364–1376 (1993).
Prescott, J. & Blackburn, E.H. Genes Dev. 11, 528–540 (1997).
Greene, E.C., Bednenko, J. & Shippen, D.E. Mol. Cell. Biol. 18, 1544–1552 (1998).
Melek, M., Greene, E.C. & Shippen, D.E. Mol. Cell. Biol. 16, 3437–3445 (1996).
Gilley, D., Lee, M.S. & Blackburn, E.H. Genes Dev. 9, 2214–2226 (1995).
Miller, M.C., Liu, J.K. & Collins, K. EMBO J. in the press (2000).
Friedman, K.L. & Cech, T.R. Genes Dev. 13, 2863–2874 (1999).
Xia, J., Peng, Y., Mian, S. & Lue, N.F. Mol. Cell. Biol. 20, 5196–5207 (2000).
Prescott, J. & Blackburn, E.H. Genes Dev. 11, 2790–2800 (1997).
Autexier, C. & Greider, C.W. Nucleic Acids Res. 26, 787–795 (1998).
Beattie, T.L., Zhou, W., Robinson, M.O. & Harrington, L. Curr. Biol. 8, 177–180 (1998).
Tesmer, V.M. et al. Mol. Cell. Biol. 19, 6207–6216 (1999).
Gilley, D. & Blackburn, E.H. Mol. Cell. Biol. 16, 66–75 (1996).
Autexier, C. & Greider, C.W. Genes Dev. 8, 563–575 (1994).
Autexier, C. & Greider, C.W. Genes Dev. 9, 2227–2239 (1995).
Fulton, T.B. & Blackburn, E.H. Mol. Cell. Biol. 18, 4961–4970 (1998).
Tzfati, Y., Fulton, T.B., Roy, J. & Blackburn, E.H. Science 288, 863–867 (2000).
Bhattacharyya, A. & Blackburn, E.H. Proc. Natl. Acad. Sci. USA 94, 2823–2827 (1997).
Ware, T.L., Wang, H. & Blackburn, E.H. EMBO J. 19, 3119–3131 (2000).
Eickbush, T.H. Science 277, 911–912 (1997).
Saldanha, R. et al. Biochemistry 38, 9069–9083 (1999).
Eickbush, D.G., Luan, D.D. & Eickbush, T.H. Mol. Cell. Biol. 20, 213–223 (2000).
Zimmerly, S., Moran, J.V., Perlman, P.S. & Lambowitz, A.M. J. Mol. Biol. 289, 473–490 (1999).
Tavis, J.E. & Ganem, D. J. Virol. 70, 5741–5750 (1996).
Mujeeb, A., Clever, J.L., Billeci, T.M., James, T.L. & Parslow, T.G. Nature Struct. Biol. 5, 432–436 (1998).
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Blackburn, E. The end of the (DNA) line. Nat Struct Mol Biol 7, 847–850 (2000). https://doi.org/10.1038/79594
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DOI: https://doi.org/10.1038/79594
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