Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

From crystal clear ligand binding to designer I domains

Nature Structural Biology volume 7, pages 614–616 (2000)Cite this article

A long awaited crystal structure of an integrin I domain in complex with a peptide derived from collagen has revealed the ligand-bound conformation of this domain and suggests a mechanism for allosteric control of integrin function by ligand binding. Also, a computational protein design approach has allowed the creation of stable, high affinity forms of the I domain for the first time.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Schematic view of the changes in metal ion coordination of the α2 I domain MIDAS motif upon ligand binding.
Figure 2: Open and closed conformations of the α2 and Mac-1 I domains.
Figure 3: Schematic diagram of integrin domain structure and hypothetical model of integrin allostery1,23,26,27,28,29,30.

References

  1. Emsley, J., Knight, C.G., Farndale, R.W., Barnes, M.J. & Liddington, R.C. Cell 101, 47–56 (2000).

    Article  CAS  Google Scholar 

  2. Shimaoka, M., Shifman, J.M., Takagi, J., Mayo, S.L. & Springer, T.A. Nature Struct. Biol. 7, 674–678 (2000).

    Article  CAS  Google Scholar 

  3. Springer, T.A. Proc. Natl. Acad. Sci. USA 94, 65–72 (1997).

    Article  CAS  Google Scholar 

  4. Michishita, M., Videm, V. & Arnaout, M.A. Cell 72, 857–867 (1993).

    Article  CAS  Google Scholar 

  5. Lee, J.-O., Rieu, P., Arnaout, M.A. & Liddington, R. Cell 80, 631–638 (1995).

    Article  CAS  Google Scholar 

  6. Lambright, D.G., Noel, J.P., Hamm, H.E. & Sigler, P.B. Nature 369, 621–628 (1994).

    Article  CAS  Google Scholar 

  7. Lee, J.-O., Bankston, L.A., Arnaout, M.A. & Liddington, R.C. Structure 3, 1333–1340 (1995).

    Article  CAS  Google Scholar 

  8. Qu, A. & Leahy, D.J. Proc. Natl. Acad. Sci. USA 92, 10277–10281 (1995).

    Article  CAS  Google Scholar 

  9. Qu, A. & Leahy, D.J. Structure 4, 931–942 (1996).

    Article  CAS  Google Scholar 

  10. Emsley, J., King, S.L., Bergelson, J.M. & Liddington, R.C. J. Biol. Chem. 272, 28512–28517 (1997).

    Article  CAS  Google Scholar 

  11. Rich, R.L. et al. J. Biol. Chem. 274, 24906–24913 (1999).

    Article  CAS  Google Scholar 

  12. Baldwin, E.T. et al. Structure 6, 923–935 (1998).

    Article  CAS  Google Scholar 

  13. Li, R., Rieu, P., Griffith, D.L., Scott, D. & Arnaout, M.A. J. Cell Biol. 143, 1523–1534 (1998).

    Article  CAS  Google Scholar 

  14. Dickeson, S.K., Bhattacharyya-Pakrasi, M., Mathis, N.L., Schlesinger, P.H. & Santoro, S.A. Biochemistry 37, 11280–11288 (1998).

    Article  CAS  Google Scholar 

  15. Huth, J.R. et al. Proc. Natl. Acad. Sci. U S A 97, 5231–5236 (2000).

    Article  CAS  Google Scholar 

  16. McDowall, A., Leitinger, B., Stanley, P., Bates, P.A., Randi, A. & Hogg, N. J. Biol. Chem. 273, 27396–27403 (1998).

    Article  Google Scholar 

  17. Zhang, L. & Plow, E.F. J. Biol. Chem. 271, 29953–29957 (1996).

    Article  CAS  Google Scholar 

  18. Kallen, J. et al. J. Mol. Biol. 292, 1–9 (1999).

    Article  CAS  Google Scholar 

  19. Oxvig, C., Lu, C. & Springer, T.A. Proc. Natl. Acad. Sci. USA 96, 2215–2220 (1999).

    Article  CAS  Google Scholar 

  20. Dahiyat, B.I. & Mayo, S.L. Science 278, 82–87 (1997).

    Article  CAS  Google Scholar 

  21. Irie, A., Kamata, T. & Takada, Y. Proc. Natl. Acad. Sci. USA 94, 7198–7203 (1997).

    Article  CAS  Google Scholar 

  22. Cierniewski, C.S. et al. J. Biol. Chem. 274, 16923–16932 (1999).

    Article  CAS  Google Scholar 

  23. Hantgan, R.R., Paumi, C., Rocco, M. & Weisel, J.W. Biochemistry 38, 14461–14474 (1999).

    Article  CAS  Google Scholar 

  24. Stephens, P.E., Ortlepp, S., Perkins, V.C., Robinson, M.K. & Kirby, H. Cell Adhes. Commun. 7, 377–390 (2000).

    Article  CAS  Google Scholar 

  25. Pampori, N. et al., J. Biol. Chem. 274, 21609–21616 (1999).

    Article  CAS  Google Scholar 

  26. Puzon-McLaughlin, W., Kamata, T. & Takada, Y. J. Biol. Chem. 275, 7795–7802 (2000).

    Article  CAS  Google Scholar 

  27. Zang, Q., Lu, C., Huang, C., Takagi, J. & Springer, T.A. J. Biol. Chem., in press (2000).

  28. Hughes, P.E. et al. J. Biol. Chem. 271, 6571–6574 (1996).

    Article  CAS  Google Scholar 

  29. Vinogradova, O., Haas, T., Plow, E.F. & Qin, J. Proc. Natl. Acad. Sci. U S A 97, 1450–1455 (2000).

    Article  CAS  Google Scholar 

  30. Bork, P., Doerks, T., Springer, T.A. & Snel, B. Trends Biochem. Sci. 24, 261–263 (1999).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    Birgit Leitinger & Nancy Hogg

Authors
  1. Birgit Leitinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nancy Hogg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Birgit Leitinger or Nancy Hogg.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Leitinger, B., Hogg, N. From crystal clear ligand binding to designer I domains. Nat Struct Mol Biol 7, 614–616 (2000). https://doi.org/10.1038/77895

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/77895

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing