Abstract
GroEL is thought to assist protein folding in part by providing a passive 'box' in which folding can occur without danger of aggregation. Two new papers now suggest that GroEL can also play a more active role by promoting rearrangement and even unfolding of kinetic folding intermediates.
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References
Sigler, P.B. et al. Annu. Rev. Biochem. 67, 581–608 (1998).
Rye, H.S. et al. Cell 97, 325–38 (1999).
Peralta, D., Hartman, D.J., Hoogenraad, N.J. & Hoj, P.B. FEBS Lett. 339, 45–49 (1994).
Sparrer, H., Rutkat, K. & Buchner, J. Proc. Natl. Acad. Sci. USA 94, 1096–100 (1997).
Todd, M.J., Viitanen, P.V. & Lorimer, G.H. Science 265, 659–666 (1994).
Coyle, J.E. et al. Nature Struct. Biol. 6, 683–690 (1999).
Shtilerman, M., Lorimer, G.H. & Englander, S.W. Science 284, 822–825 (1999).
Radford, S.E. & Dobson, C.M. Phil. Trans. R. Soc. Lond. B 348, 17–25 (1995).
Ewalt, K.L., Hendrick, J.P., Houry, W.A. & Hartl, F.U. Cell 90, 491–500 (1997).
Lorimer, G.H. FASEB J. 10, 5–9 (1996).
Ellis, R.J. & Hartl, F.U. FASEB J. 10, 20–26 (1996).
Dill, K.A. & Chan, H.S. Nature Struct. Biol. 4, 10–9 (1997).
Sosnick, T.R., Mayne, L., Hiller, R. & Englander, S.W. Nature Struct. Biol. 1, 149–156 (1994).
Weissman, J.S. & Kim, P.S. Science 253, 1386–1393 (1991).
Todd, M.J., Lorimer, G.H. & Thirumalai, D. Proc. Natl. Acad. Sci. USA 93, 4030–5 (1996).
Corrales, F.J. & Fersht, A.R. Proc. Natl. Acad. Sci. USA 93, 4509–12 (1996).
Goloubinoff, P., Gatenby, A.A. & Lorimer, G.H. Nature 337, 44–47 (1989).
Goloubinoff, P., Christeller, J.T., Gatenby, A.A. & Lorimer, G.H. Nature 342, 884–889 (1989).
Weissman, J.S., Kashi, Y., Fenton, W.A. & Horwich, A.L. Cell 78, 693–702 (1994).
Zahn, R., Perrett, S., Stenberg, G. & Fersht, A.R. Science 271, 642–645 (1996).
Walter, S., Lorimer, G.H. & Schmid, F.X. Proc. Natl. Acad. Sci. USA 93, 9425–30 (1996).
Weissman, J.S., Rye, H.S., Fenton, W.A., Beechem, J.M. & Horwich, A.L. Cell 84, 481–490 (1996).
Rye, H.S. et al. Nature 388, 792–8 (1997).
Braig, K. et al. Nature 371, 578–586 (1994).
Xu, Z., Horwich, A.L. & Sigler, P.B. Nature 388, 741–50 (1997).
Acknowledgements
We thank G. Lorimer and P. Sigler for Fig. 3, and members of the Weissman lab for helpful comments.
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Wang, J., Weissman, J. Thinking outside the box: new insights into the mechanism of GroEL-mediated protein folding. Nat Struct Mol Biol 6, 597–600 (1999). https://doi.org/10.1038/10636
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DOI: https://doi.org/10.1038/10636
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