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Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms

Nature Structural Biology volume 5, pages 347–351 (1998)Cite this article

Abstract

The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 Å resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.

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Author notes

  1. Shigehiro Nagashima

    Present address: Namba Protonic Nanomachine Project, ERATO, JST, Hikaridai 3-4, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan

  2. Shigehiro Nagashima, Masayoshi Nakasako, Naoshi Dohmae, Masanari Tsujimura, Koji Takio, Masafumi Odaka, Masafumi Yohda, Nobuo Kamiya and Isao Endo: All of the authors contributed equally to this work.

Authors and Affiliations

  1. The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    Shigehiro Nagashima, Naoshi Dohmae, Masanari Tsujimura, Koji Takio, Masafumi Odaka, Masafumi Yohda, Nobuo Kamiya & Isao Endo

  2. PRESTO, JST and Institute for Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-0032, Japan

    Masayoshi Nakasako

  3. Graduate School of Science and Engineering, Saitama University, Shimo-Okubo 251, Urawa, Saitama, 338-0825, Japan

    Masanari Tsujimura

  4. TARA Sakabe Project, University of Tsukuba, Tennodai 1-1-1, Tsukuba-city, Ibaraki, 305-0006, Japan

    Nobuo Kamiya

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  1. Shigehiro Nagashima
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Correspondence to Isao Endo.

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Nagashima, S., Nakasako, M., Dohmae, N. et al. Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nat Struct Mol Biol 5, 347–351 (1998). https://doi.org/10.1038/nsb0598-347

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  • DOI: https://doi.org/10.1038/nsb0598-347

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