Correspondence | Published:

Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli

The structure of an inactive mutant, heat-labile enterotoxin raises the possibility of a direct functional role for an internal, water-filled cavity.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Cassel, D. & Pfeuffer, T. Proc. natn. Acad. Sci. U.S.A. 75, 2669–2673 (1978).

  2. 2

    Sixma, T.K et al. Nature 351, 371–378 (1991).

  3. 3

    Sixma, T.K. et al. J. molec. Biol. 230, 890–918 (1993).

  4. 4

    Domenighini, M., Montecucco, C., Ripka, W.C. & Rappuoli, R. Molec. Microbiol. 5, 23–31 (1991).

  5. 5

    Burnette, W.N. et al. Infect. Immun. 59, 4266–4270 (1991).

  6. 6

    Lobet, Y., Cluff, C.W. & Cieplak, W., Infect. Immun. 59, 2870–2879 (1991).

  7. 7

    Harford, S., Dykes, C.W., Hobden, A.N., Read, M.J. & Halliday, I.J. Eur. J. Biochem. 183, 311–316 (1989).

  8. 8

    Pizza, M. et al. Molec. Microbiol. 14, 51–60 (1994).

  9. 9

    Moss, J., Stanley, S., Vaughan, M. & Tsuji, T. J. biol. Chem. 268, 6383–6387 (1993).

  10. 10

    Williams, M.A., Goodfellow, J.M. & Thornton, J.M. Prof. Sci. 3, 1224–1235 (1994).

  11. 11

    Pronk, S.E. et al. J. biol.Chem. 260, 13580–13584 (1987).

  12. 12

    CCP4 The SERC (UK) collaborative computing project no. 4, a suite of programs for protein crystallograpriy(Daresbury Laboratory, Warrington WA4 4AD, UK; 1979).

  13. 13

    Brünger, A.T. X-PLOR Version 2.1 Manual. (Yale University; 1990).

  14. 14

    Kraulis, P. J. appl. Crystallogr. 24, 946–950 (1991).

  15. 15

    Bacon, D.J. & Anderson, W.F. J. molec. Graphics 6, 219–220 (1988).

  16. 16

    Merritt, E.A. & Murphy, M.E.P. Acta crystallogr. D50, 869–873 (1994).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article