The growth of three–dimensional protein crystals is seeded by two–dimensional crystals formed on lipid layers. Such crystallization occurs faster and at lower precipitant and protein concentrations than conventional crystal growth. This approach may also allow the crystallization of proteins that resist attempts at crystal growth by other means.
This is a preview of subscription content, access via your institution
Subscribe to Journal
Get full journal access for 1 year
only $8.25 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
McPherson, A. in Crystallization of Membrane Proteins (ed. Michel, H.) 1–51 (CRC Press, Boca Raton, Florida, 1991).
McPherson, A. & Shichta, P. Heterogeneous and epitaxial nudeation of protein crystals on mineral surfaces. Science, 385–387 (1988).
Uzgiris, E.E. & Kornberg, R.D. Two-dimensional crystalllization techniques for imaging macromolecules, with an application to antigen-antibody-complement complexes. Nature 301, 125–129 (1983).
Kornberg, R.D. & Darst, S.A. Two-dimensional crystals of proteins on lipid layers. Curr. Opin. struct. Biol. 1, 642–646 (1991).
Ku, A.C., Darst, S.A., Kornberg, R.D., Robertson, C.R. & Gast, A.P. Dendritic growth of two-dimensional protein crystals. Langmuir 8, 2357–2360 (1992).
Kubalek, E.W., Darst, S.A. & Kornberg, R.D. Improved transfer of two-dimensional crystals from the air/water interface to specimen support grids for high-resolution analysis by electron microscopy. Ultramicroscopy 35, 295–304 (1991).
Darst, S.A., Kubalek, E.W., Edwards, A.E. & Kornberg, R.D. Two-dimensional and epitaxial crystallization of a mutant form of yeast RNA polymerase II. J. molec. Biol. 221, 347–357 (1991).
Pahler, A., Hendrickson, W., Kolks, M., Argarana, C. & Cantor, C. Characterization and crystallization of core streptavidin. J. biol. Chem. 262, 13933–13937 (1987).
Weber, P.C., Cox, M.J., Salemme, F.R. & Ohlendorf, D.H. Crystallographic data for Strepfomyces avidnii strewptavidin. J. biol. Chem. 262, 12728–12729 (1987).
Young, R.A. Polymerase II. Rev. Biochem. 60, 689–715 (1991).
Thuriax, P. & Sentenac, A. In The molecular and cellular biology of the yeast saccharomyces: gene expression. II, 1–48 (Cold Spring Harbor Laboratory Press, New York, 1992).
Frappier, L. & O'Donnell, M. Overproduction, purification and characterization of EBNA1, the origin binding protein of Epstein-Barr virus J. biol. Chem. 266, 7819–7826 (1991).
About this article
Cite this article
Edward, A., Darst, S., Hemming, S. et al. Epitaxial growth of protein crystals on lipid layers. Nat Struct Mol Biol 1, 195–197 (1994). https://doi.org/10.1038/nsb0394-195