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The impact of insulin resistance on the kidney and vasculature

Key Points

  • In addition to classical insulin target tissues (liver, skeletal muscle and white adipose tissue) insulin acts on most human organs and cell types, including the arterial vasculature and the kidney

  • In insulin-resistant states such as obesity or type 2 diabetes mellitus, not only are the classical insulin effects impaired, but also the effects of insulin on the vasculature and the kidney

  • Insulin stimulates its own delivery to target cells by actions on the vasculature involving increased capillary recruitment and endothelial transcytosis; these effects are impaired in insulin-resistant states

  • Insulin resistance affects many aspects of kidney function, including renal haemodynamics, podocyte viability and tubular function

  • The action of insulin on renal sodium handling is preserved in insulin resistance and contributes to sodium retention and arterial hypertension

  • Renal and vascular insulin resistance can be improved through an integrated approach including lifestyle interventions and pharmacological agents

Abstract

Insulin resistance is a systemic disorder that affects many organs and insulin-regulated pathways. The disorder is characterized by a reduced action of insulin despite increased insulin concentrations (hyperinsulinaemia). The effects of insulin on the kidney and vasculature differ in part from the effects on classical insulin target organs. Insulin causes vasodilation by enhancing endothelial nitric oxide production through activation of the phosphatidylinositol 3-kinase pathway. In insulin-resistant states, this pathway is impaired and the mitogen-activated protein kinase pathway stimulates vasoconstriction. The action of insulin on perivascular fat tissue and the subsequent effects on the vascular wall are not fully understood, but the hepatokine fetuin-A, which is released by fatty liver, might promote the proinflammatory effects of perivascular fat. The strong association of salt-sensitive arterial hypertension with insulin resistance indicates an involvement of the kidney in the insulin resistance syndrome. The insulin receptor is expressed on renal tubular cells and podocytes and insulin signalling has important roles in podocyte viability and tubular function. Renal sodium transport is preserved in insulin resistance and contributes to the salt-sensitivity of blood pressure in hyperinsulinaemia. Therapeutically, renal and vascular insulin resistance can be improved by an integrated holistic approach aimed at restoring overall insulin sensitivity and improving insulin signalling.

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Figure 1: The insulin signalling pathway.
Figure 2: Organ crosstalk in the insulin-resistant state.
Figure 3: The effects of insulin on the arterial vasculature.
Figure 4: Transendothelial transport of insulin in a skeletal muscle capillary.
Figure 5: Insulin signal transduction in the endothelial cells of resistance arterioles.
Figure 6: Perivascular adipose tissue influences vascular function.
Figure 7: Insulin signalling in the principal cell of the aldosterone-sensitive distal nephron.

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References

  1. Cuatrecasas, P. The insulin receptor. Diabetes 21, 396–402 (1972).

    Article  CAS  PubMed  Google Scholar 

  2. Olefsky, J. M. Insulin binding, biologic activity, and metabolism of biosynthetic human insulin. Diabetes Care 4, 244–247 (1981).

    Article  CAS  PubMed  Google Scholar 

  3. Kahn, C. R., Neville, D. M. Jr & Roth, J. Insulin-receptor interaction in the obese-hyperglycemic mouse. A model of insulin resistance. J. Biol. Chem. 248, 244–250 (1973).

    CAS  PubMed  Google Scholar 

  4. Groop, L. C. et al. Glucose and free fatty acid metabolism in non-insulin-dependent diabetes mellitus. Evidence for multiple sites of insulin resistance. J. Clin. Invest. 84, 205–213 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Prager, R., Wallace, P. & Olefsky, J. M. In vivo kinetics of insulin action on peripheral glucose disposal and hepatic glucose output in normal and obese subjects. J. Clin. Invest. 78, 472–481 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Rask-Madsen, C. & Kahn, C. R. Tissue-specific insulin signaling, metabolic syndrome, and cardiovascular disease. Arterioscler Thromb. Vasc. Biol. 32, 2052–2059 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Heni, M., Kullmann, S., Preissl, H., Fritsche, A. & Haring, H. U. Impaired insulin action in the human brain: causes and metabolic consequences. Nat. Rev. Endocrinol. 11, 701–711 (2015). This review summarizes the current knowledge of normal and impaired cerebral insulin effects.

    Article  CAS  PubMed  Google Scholar 

  8. Kellerer, M. et al. Distinct alpha-subunit structures of human insulin receptor A and B variants determine differences in tyrosine kinase activities. Biochemistry 31, 4588–4596 (1992).

    Article  CAS  PubMed  Google Scholar 

  9. Seino, S. & Bell, G. I. Alternative splicing of human insulin receptor messenger RNA. Biochem. Biophys. Res. Commun. 159, 312–316 (1989).

    Article  CAS  PubMed  Google Scholar 

  10. Belfiore, A., Frasca, F., Pandini, G., Sciacca, L. & Vigneri, R. Insulin receptor isoforms and insulin receptor/insulin-like growth factor receptor hybrids in physiology and disease. Endocr. Rev. 30, 586–623 (2009).

    Article  CAS  PubMed  Google Scholar 

  11. Kasuga, M., Karlsson, F. A. & Kahn, C. R. Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor. Science 215, 185–187 (1982).

    Article  CAS  PubMed  Google Scholar 

  12. Backer, J. M. et al. Phosphatidylinositol 3′-kinase is activated by association with IRS-1 during insulin stimulation. EMBO J. 11, 3469–3479 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Sun, X. J. et al. Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein. Nature 352, 73–77 (1991).

    Article  CAS  PubMed  Google Scholar 

  14. Taniguchi, C. M., Emanuelli, B. & Kahn, C. R. Critical nodes in signalling pathways: insights into insulin action. Nat. Rev. Mol. Cell Biol. 7, 85–96 (2006).

    Article  CAS  PubMed  Google Scholar 

  15. Farese, R. V., Sajan, M. P. & Standaert, M. L. Atypical protein kinase C in insulin action and insulin resistance. Biochem. Soc. Trans. 33, 350–353 (2005).

    Article  CAS  PubMed  Google Scholar 

  16. Manning, B. D. & Cantley, L. C. AKT/PKB signaling: navigating downstream. Cell 129, 1261–1274 (2007).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Brady, M. J. & Saltiel, A. R. The role of protein phosphatase-1 in insulin action. Recent Prog. Horm. Res. 56, 157–173 (2001).

    Article  CAS  PubMed  Google Scholar 

  18. Elchebly, M. et al. Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene. Science 283, 1544–1548 (1999).

    Article  CAS  PubMed  Google Scholar 

  19. Lazar, D. F. & Saltiel, A. R. Lipid phosphatases as drug discovery targets for type 2 diabetes. Nat. Rev. Drug Discov. 5, 333–342 (2006).

    Article  CAS  PubMed  Google Scholar 

  20. Vinciguerra, M. & Foti, M. PTEN and SHIP2 phosphoinositide phosphatases as negative regulators of insulin signalling. Arch. Physiol. Biochem. 112, 89–104 (2006).

    Article  CAS  PubMed  Google Scholar 

  21. Emanuelli, B. et al. SOCS-3 is an insulin-induced negative regulator of insulin signaling. J. Biol. Chem. 275, 15985–15991 (2000).

    Article  CAS  PubMed  Google Scholar 

  22. Holt, L. J. & Siddle, K. Grb10 and Grb14: enigmatic regulators of insulin action—and more? Biochem. J. 388, 393–406 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Copps, K. D. & White, M. F. Regulation of insulin sensitivity by serine/threonine phosphorylation of insulin receptor substrate proteins IRS1 and IRS2. Diabetologia 55, 2565–2582 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Fritsche, L. et al. Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907. Am. J. Physiol. Endocrinol. Metab. 300, E824–836 (2011).

    Article  CAS  PubMed  Google Scholar 

  25. Neukamm, S. S. et al. Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. J. Biol. Chem. 288, 16403–16415 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Weigert, C. et al. Interplay and effects of temporal changes in the phosphorylation state of serine-302, -307, and -318 of insulin receptor substrate-1 on insulin action in skeletal muscle cells. Mol. Endocrinol. 22, 2729–2740 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Weigert, C. et al. The phosphorylation of Ser318 of insulin receptor substrate 1 is not per se inhibitory in skeletal muscle cells but is necessary to trigger the attenuation of the insulin-stimulated signal. J. Biol. Chem. 280, 37393–37399 (2005). This study shows the complex molecular regulation of the function of insulin receptor substrate 1 by specific serine phosphorylation.

    Article  CAS  PubMed  Google Scholar 

  28. Boucher, J., Kleinridders, A. & Kahn, C. R. Insulin receptor signaling in normal and insulin-resistant states. Cold Spring Harb Perspect Biol 6 a009191 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Kowluru, A. & Matti, A. Hyperactivation of protein phosphatase 2A in models of glucolipotoxicity and diabetes: potential mechanisms and functional consequences. Biochem. Pharmacol. 84, 591–597 (2012).

    Article  CAS  PubMed  Google Scholar 

  30. Vaidyanathan, K. & Wells, L. Multiple tissue-specific roles for the O-GlcNAc post-translational modification in the induction of and complications arising from type II diabetes. J. Biol. Chem. 289, 34466–34471 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Potenza, M. A., Addabbo, F. & Montagnani, M. Vascular actions of insulin with implications for endothelial dysfunction. Am. J. Physiol. Endocrinol. Metab. 297, E568–577 (2009).

    Article  CAS  PubMed  Google Scholar 

  32. Hale, L. J. & Coward, R. J. The insulin receptor and the kidney. Curr. Opin. Nephrol. Hypertens. 22, 100–106 (2013).

    Article  CAS  PubMed  Google Scholar 

  33. Coward, R. J. et al. The human glomerular podocyte is a novel target for insulin action. Diabetes 54, 3095–3102 (2005).

    Article  CAS  PubMed  Google Scholar 

  34. Conti, F. G. et al. Studies on binding and mitogenic effect of insulin and insulin-like growth factor I in glomerular mesangial cells. Endocrinology 122, 2788–2795 (1988).

    Article  CAS  PubMed  Google Scholar 

  35. Conti, F. G., Elliot, S. J., Striker, L. J. & Striker, G. E. Binding of insulin-like growth factor-I by glomerular endothelial and epithelial cells: further evidence for IGF-I action in the renal glomerulus. Biochem. Biophys. Res. Commun. 163, 952–958 (1989).

    Article  CAS  PubMed  Google Scholar 

  36. Nakamura, R., Emmanouel, D. S. & Katz, A. I. Insulin binding sites in various segments of the rabbit nephron. J. Clin. Invest. 72, 388–392 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Ejerblad, E. et al. Obesity and risk for chronicrenal failure. J. Am. Soc. Nephrol. 17, 1695–1702 (2006).

    Article  CAS  PubMed  Google Scholar 

  38. Fox, C. S. et al. Predictors of new-onset kidney disease in a community-based population. Jama 291, 844–850 (2004).

    Article  CAS  PubMed  Google Scholar 

  39. Kanasaki, K., Kitada, M., Kanasaki, M. & Koya, D. The biological consequence of obesity on the kidney. Nephrol. Dial. Transplant 28, (Suppl. 4), 1–7 (2013).

    Google Scholar 

  40. Pinto-Sietsma, S. J. et al. A central body fat distribution is related to renal function impairment, even in lean subjects. Am. J. Kidney Dis. 41, 733–741 (2003).

    Article  PubMed  Google Scholar 

  41. Ritz, E. Metabolic syndrome and kidney disease. Blood Purif. 26, 59–62 (2008).

    Article  CAS  PubMed  Google Scholar 

  42. Kramer, H. et al. Waist Circumference, Body Mass Index, and ESRD in the REGARDS (Reasons for Geographic and Racial Differences in Stroke) Study. Am. J. Kidney Dis. 67, 62–69 (2016).

    Article  PubMed  Google Scholar 

  43. Chandie Shaw, P. K. et al. Central obesity is an independent risk factor for albuminuria in nondiabetic South Asian subjects. Diabetes Care 30, 1840–1844 (2007).

    Article  PubMed  Google Scholar 

  44. Cirillo, M. et al. Microalbuminuria in nondiabetic adults: relation of blood pressure, body mass index, plasma cholesterol levels, and smoking: The Gubbio Population Study. Arch. Intern. Med. 158, 1933–1939 (1998).

    Article  CAS  PubMed  Google Scholar 

  45. Tozawa, M. et al. Influence of smoking and obesity on the development of proteinuria. Kidney Int. 62, 956–962 (2002).

    Article  PubMed  Google Scholar 

  46. Nerpin, E. et al. Insulin sensitivity measured with euglycemic clamp is independently associated with glomerular filtration rate in a community-based cohort. Diabetes Care 31, 1550–1555 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

  47. De Cosmo, S., Menzaghi, C., Prudente, S. & Trischitta, V. Role of insulin resistance in kidney dysfunction: insights into the mechanism and epidemiological evidence. Nephrol. Dial. Transplant 28, 29–36 (2013).

    Article  CAS  PubMed  Google Scholar 

  48. Saltiel, A. R. & Kahn, C. R. Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414, 799–806 (2001).

    Article  CAS  PubMed  Google Scholar 

  49. Karlsson, F. H. et al. Gut metagenome in European women with normal, impaired and diabetic glucose control. Nature 498, 99–103 (2013).

    Article  CAS  PubMed  Google Scholar 

  50. Odegaard, J. I. & Chawla, A. Pleiotropic actions of insulin resistance and inflammation in metabolic homeostasis. Science 339, 172–177 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Adamczak, M. & Wiecek, A. The adipose tissue as an endocrine organ. Semin. Nephrol. 33, 2–13 (2013).

    Article  CAS  PubMed  Google Scholar 

  52. Sharma, K. et al. Adiponectin regulates albuminuria and podocyte function in mice. J. Clin. Invest. 118, 1645–1656 (2008).

    CAS  PubMed  PubMed Central  Google Scholar 

  53. Wolf, G. et al. Leptin stimulates proliferation and TGF-beta expression in renal glomerular endothelial cells: potential role in glomerulosclerosis [seecomments]. Kidney Int. 56, 860–872 (1999).

    Article  CAS  PubMed  Google Scholar 

  54. Nerlich, A. G., Schleicher, E. D., Wiest, I., Specks, U. & Timpl, R. Immunohistochemical localization of collagen VI in diabetic glomeruli. Kidney Int. 45, 1648–1656 (1994).

    Article  CAS  PubMed  Google Scholar 

  55. Stefan, N. et al. Obesity and renal disease: not all fat is created equal and not all obesity is harmful to the kidneys. Nephrol. Dial. Transplant 56, 860–872 (2014).

    Google Scholar 

  56. Stefan, N. & Haring, H. U. The role of hepatokines in metabolism. Nat. Rev. Endocrinol. 9, 144–152 (2013).

    Article  CAS  PubMed  Google Scholar 

  57. Stefan, N., Haring, H. U., Hu, F. B. & Schulze, M. B. Metabolically healthy obesity: epidemiology, mechanisms, and clinical implications. Lancet Diabetes Endocrinol. 1, 152–162 (2013).

    Article  PubMed  Google Scholar 

  58. Stefan, N. et al. Identification and characterization of metabolically benign obesity in humans. Arch. Intern. Med. 168, 1609–1616 (2008).

    Article  PubMed  Google Scholar 

  59. Haukeland, J. W. et al. Fetuin A in nonalcoholic fatty liver disease: in vivo and in vitro studies. Eur. J. Endocrinol. 166, 503–510 (2012).

    Article  CAS  PubMed  Google Scholar 

  60. Lehmann, R. et al. Circulating lysophosphatidylcholines are markers of a metabolically benign nonalcoholic fatty liver. Diabetes Care 36, 2331–2338 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  61. Stefan, N. & Haring, H. U. The metabolically benign and malignant fatty liver. Diabetes 60, 2011–2017 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. Stefan, N. et al. Alpha2-Heremans-Schmid glycoprotein/fetuin-A is associated with insulin resistance and fat accumulation in the liver in humans. Diabetes Care 29, 853–857 (2006).

    Article  CAS  PubMed  Google Scholar 

  63. Auberger, P. et al. Characterization of a natural inhibitor of the insulin receptor tyrosine kinase: cDNA cloning, purification, and anti-mitogenic activity. Cell 58, 631–640 (1989).

    Article  CAS  PubMed  Google Scholar 

  64. Hennige, A. M. et al. Fetuin-A induces cytokine expression and suppresses adiponectin production. PLoS ONE 3, e1765 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Ix, J. H. et al. Fetuin-A and incident diabetes mellitus in older persons. Jama 300, 182–188 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Stefan, N. et al. Plasma fetuin-A levels and the risk of type 2 diabetes. Diabetes 57, 2762–2767 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Fisher, E. et al. Association of AHSG gene polymorphisms with fetuin-A plasma levels and cardiovascular diseases in the EPIC-Potsdam study. Circ. Cardiovasc. Genet. 2, 607–613 (2009).

    Article  CAS  PubMed  Google Scholar 

  68. Weikert, C. et al. Plasma fetuin-a levels and the risk of myocardial infarction and ischemic stroke. Circulation 118, 2555–2562 (2008).

    Article  CAS  PubMed  Google Scholar 

  69. Pal, D. et al. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat. Med. 18, 1279–1285 (2012).

    Article  CAS  PubMed  Google Scholar 

  70. Stefan, N. & Haring, H. U. Circulating fetuin-A and free fatty acids interact to predict insulin resistance in humans. Nat. Med. 19, 394–395 (2013).

    Article  CAS  PubMed  Google Scholar 

  71. Stefan, N., Schick, F. & Haring, H. U. Ectopic fat in insulin resistance, dyslipidemia, and cardiometabolic disease. N. Engl. J. Med. 371, 2236–2237 (2014).

    Article  PubMed  Google Scholar 

  72. Schafer, C. et al. The serum protein alpha 2-Heremans-Schmid glycoprotein/fetuin-A is a systemically acting inhibitor of ectopic calcification. J. Clin. Invest. 112, 357–366 (2003).

    Article  PubMed  PubMed Central  Google Scholar 

  73. Li, M. et al. Association between higher serum fetuin-A concentrations and abnormal albuminuria in middle-aged and elderly chinese with normal glucose tolerance. Diabetes Care 33, 2462–2464 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. Page, M. M. & Watkins, P. J. Provocation of postural hypotension by insulin in diabetic autonomic neuropathy. Diabetes 25, 90–95 (1976).

    Article  CAS  PubMed  Google Scholar 

  75. Baron, A. D. Hemodynamic actions of insulin. Am. J. Physiol. 267, E187–E202 (1994).

    CAS  PubMed  Google Scholar 

  76. Laakso, M., Edelman, S. V., Brechtel, G. & Baron, A. D. Decreased effect of insulin to stimulate skeletal muscle blood flow in obese man. A novel mechanism for insulin resistance. J. Clin. Invest. 85, 1844–1852 (1990). This study provides experimental evidence for insulin-mediated vasodilation and its increasing impairment in patients with insulin-resistance and diabetes.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Laakso, M. et al. Kinetics of in vivo muscle insulin-mediated glucose uptake in human obesity. Diabetes 39, 965–974 (1990).

    Article  CAS  PubMed  Google Scholar 

  78. Kim, J. A., Montagnani, M., Koh, K. K. & Quon, M. J. Reciprocal relationships between insulin resistance and endothelial dysfunction: molecular and pathophysiological mechanisms. Circulation 113, 1888–1904 (2006).

    Article  PubMed  Google Scholar 

  79. Jahn, L. A. et al. Insulin enhances endothelial function throughout the arterial tree in healthy but not metabolic syndrome subjects. J. Clin. Endocrinol. Metab. 101, 1198–1206 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  80. Steinberg, H. O., Brechtel, G., Johnson, A., Fineberg, N. & Baron, A. D. Insulin-mediated skeletal muscle vasodilation is nitric oxide dependent. A novel action of insulin to increase nitric oxide release. J. Clin. Invest. 94, 1172–1179 (1994). This study showed for the first time that insulin effects on all levels of the vascular tree are impaired in patients with insulin resistance.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  81. Jialal, I. et al. Characterization of the receptors for insulin and the insulin-like growth factors on micro- and macrovascular tissues. Endocrinology 117, 1222–1229 (1985).

    Article  CAS  PubMed  Google Scholar 

  82. Montero, D. Hemodynamic actions of insulin: beyond the endothelium. Front. Physiol. 4, 389 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  83. King, G. L. & Johnson, S. M. Receptor-mediated transport of insulin across endothelial cells. Science 227, 1583–1586 (1985).

    Article  CAS  PubMed  Google Scholar 

  84. Kubota, T. et al. Impaired insulin signaling in endothelial cells reduces insulin-induced glucose uptake by skeletal muscle. Cell Metab. 13, 294–307 (2011).

    Article  CAS  PubMed  Google Scholar 

  85. Azizi, P. M. et al. Clathrin-dependent entry and vesicle-mediated exocytosis define insulin transcytosis across microvascular endothelial cells. Mol. Biol. Cell 26, 740–750 (2015). This study shows the detailed molecular mechanism of insulin transcytosis through the endothelial layer.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  86. Wang, H., Wang, A. X., Aylor, K. & Barrett, E. J. Nitric oxide directly promotes vascular endothelial insulin transport. Diabetes 62, 4030–4042 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  87. Symons, J. D. et al. Contribution of insulin and Akt1 signaling to endothelial nitric oxide synthase in the regulation of endothelial function and blood pressure. Circ. Res. 104, 1085–1094 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  88. Muniyappa, R., Iantorno, M. & Quon, M. J. An integrated view of insulin resistance and endothelial dysfunction. Endocrinol. Metab. Clin. North Am. 37, 685–711, (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  89. Wang, Y. et al. APPL1 counteracts obesity-induced vascular insulin resistance and endothelial dysfunction by modulating the endothelial production of nitric oxide and endothelin-1 in mice. Diabetes 60, 3044–3054 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  90. Ryu, J. et al. APPL1 potentiates insulin sensitivity by facilitating the binding of IRS1/2 to the insulin receptor. Cell Rep. 7, 1227–1238 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  91. Du, K., Herzig, S., Kulkarni, R. N. & Montminy, M. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science 300, 1574–1577 (2003).

    Article  CAS  PubMed  Google Scholar 

  92. de Boer, M. P. et al. Globular adiponectin controls insulin-mediated vasoreactivity in muscle through AMPKα2. Vascul Pharmacol. 78, 24–35 (2016).

    Article  CAS  PubMed  Google Scholar 

  93. Dong, Z. et al. Protein kinase A mediates glucagon-like peptide 1-induced nitric oxide production and muscle microvascular recruitment. Am. J. Physiol. Endocrinol. Metab. 304, E222–E228 (2013).

    Article  CAS  PubMed  Google Scholar 

  94. Wang, B. et al. Blood pressure-lowering effects of GLP-1 receptor agonists exenatide and liraglutide: a meta-analysis of clinical trials. Diabetes Obes. Metab. 15, 737–749 (2013).

    Article  CAS  PubMed  Google Scholar 

  95. Vicent, D. et al. The role of endothelial insulin signaling in the regulation of vascular tone and insulin resistance. J. Clin. Invest. 111, 1373–1380 (2003).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  96. Duplain, H. et al. Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase. Circulation 104, 342–345 (2001).

    Article  CAS  PubMed  Google Scholar 

  97. Abe, H. et al. Hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation in mice lacking insulin receptor substrate-1. J. Clin. Invest. 101, 1784–1788 (1998).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  98. Huang, C. et al. Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155. Int. J. Mol. Med. 36, 239–248 (2015).

    Article  CAS  PubMed  Google Scholar 

  99. Hashimoto, S. et al. Insulin receptor substrate-2 (Irs2) in endothelial cells plays a crucial role in insulin secretion. Diabetes 64, 876–886 (2015).

    Article  CAS  PubMed  Google Scholar 

  100. Hayashi, K. et al. Effects of insulin on rat renal microvessels: studies in the isolated perfused hydronephrotic kidney. Kidney Int. 51, 1507–1513 (1997).

    Article  CAS  PubMed  Google Scholar 

  101. Schmetterer, L. et al. Renal and ocular hemodynamic effects of insulin. Diabetes 46, 1868–1874 (1997).

    Article  CAS  PubMed  Google Scholar 

  102. Hayashi, K. et al. Altered renal microvascular response in Zucker obese rats. Metabolism 51, 1553–1561 (2002).

    Article  CAS  PubMed  Google Scholar 

  103. Buscemi, S. et al. Intra-renal hemodynamics and carotid intima-media thickness in the metabolic syndrome. Diabetes Res. Clin. Pract. 86, 177–185 (2009).

    Article  CAS  PubMed  Google Scholar 

  104. Novikov, A. & Vallon, V. Sodium glucose cotransporter 2 inhibition in the diabetic kidney: an update. Curr. Opin. Nephrol. Hypertens. 25, 50–58 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  105. Siegel-Axel, D. I. & Haring, H. U. Perivascular adipose tissue: An unique fat compartment relevant for the cardiometabolic syndrome. Rev. Endocr. Metab. Disord. 17, 51–60 (2016). This review describes the interactions of perivascular fat at different anatomical locations on the underlying vessel wall.

    Article  CAS  PubMed  Google Scholar 

  106. Tano, J. Y., Schleifenbaum, J. & Gollasch, M. Perivascular adipose tissue, potassium channels, and vascular dysfunction. Arterioscler Thromb. Vasc. Biol. 34, 1827–1830 (2014).

    Article  CAS  PubMed  Google Scholar 

  107. Gil-Ortega, M., Somoza, B., Huang, Y., Gollasch, M. & Fernandez-Alfonso, M. S. Regional differences in perivascular adipose tissue impacting vascular homeostasis. Trends Endocrinol. Metab. 26, 367–375 (2015).

    Article  CAS  PubMed  Google Scholar 

  108. Rittig, K. et al. The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells. Diabetologia 55, 1514–1525 (2012).

    Article  CAS  PubMed  Google Scholar 

  109. Siegel-Axel, D. I. et al. Fetuin-A influences vascular cell growth and production of proinflammatory and angiogenic proteins by human perivascular fat cells. Diabetologia 57, 1057–1066 (2014).

    Article  CAS  PubMed  Google Scholar 

  110. Gao, Y. J., Lu, C., Su, L. Y., Sharma, A. M. & Lee, R. M. Modulation of vascular function by perivascular adipose tissue: the role of endothelium and hydrogen peroxide. Br. J. Pharmacol. 151, 323–331 (2007).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  111. van den Born, J. C., Hammes, H. P., Greffrath, W., van Goor, H. & Hillebrands, J. L. Gasotransmitters in Vascular Complications of Diabetes. Diabetes 65, 331–345 (2016).

    Article  CAS  PubMed  Google Scholar 

  112. Houben, A. J. et al. Perivascular fat and the microcirculation: relevance to insulin resistance, diabetes, and cardiovascular disease. Curr. Cardiovasc. Risk Rep. 6, 80–90 (2012). This article emphasises the possible roles of perivascular fat in vascular dysfunction.

    Article  CAS  PubMed  Google Scholar 

  113. Yudkin, J. S., Eringa, E. & Stehouwer, C. D. “Vasocrine” signalling from perivascular fat: a mechanism linking insulin resistance to vascular disease. Lancet 365, 1817–1820 (2005).

    Article  PubMed  Google Scholar 

  114. Rittig, K. et al. Perivascular fatty tissue at the brachial artery is linked to insulin resistance but not to local endothelial dysfunction. Diabetologia 51, 2093–2099 (2008).

    Article  CAS  PubMed  Google Scholar 

  115. de Vries, A. P. et al. Fatty kidney: emerging role of ectopic lipid in obesity-related renal disease. Lancet Diabetes Endocrinol. 2, 417–426 (2014).

    Article  CAS  PubMed  Google Scholar 

  116. Foster, M. C. et al. Fatty kidney, hypertension, and chronic kidney disease: the Framingham Heart Study. Hypertension 58, 784–790 (2011).

    Article  CAS  PubMed  Google Scholar 

  117. Lamacchia, O. et al. Para- and perirenal fat thickness is an independent predictor of chronic kidney disease, increased renal resistance index and hyperuricaemia in type-2 diabetic patients. Nephrol. Dial. Transplant. 26, 892–898 (2011).

    Article  PubMed  Google Scholar 

  118. Wagner, R. et al. Exercise-induced albuminuria is associated with perivascular renal sinus fat in individuals at increased risk of type 2 diabetes. Diabetologia 55, 2054–2058 (2012).

    Article  CAS  PubMed  Google Scholar 

  119. Hysing, J., Ostensen, J., Tolleshaug, H., Andersen, K. J. & Kiil, F. Luminal and basolateral uptake and degradation of insulin in the proximal tubules of the dog kidney. Acta Physiol. Scand. 146, 241–250 (1992).

    Article  CAS  PubMed  Google Scholar 

  120. ter Maaten, J. C. et al. Insulin's acute effects on glomerular filtration rate correlate with insulin sensitivity whereas insulin's acute effects on proximal tubular sodium reabsorption correlation with salt sensitivity in normal subjects. Nephrol. Dial. Transplant. 14, 2357–2363 (1999).

    Article  CAS  PubMed  Google Scholar 

  121. Hiromura, K., Monkawa, T., Petermann, A. T., Durvasula, R. V. & Shankland, S. J. Insulin is a potent survival factor in mesangial cells: role of the PI3-kinase/Akt pathway. Kidney Int. 61, 1312–1321 (2002).

    Article  CAS  PubMed  Google Scholar 

  122. Foutz, R. M., Grimm, P. R. & Sansom, S. C. Insulin increases the activity of mesangial BK channels through MAPK signaling. Am. J. Physiol. Renal Physiol. 294, F1465–1472 (2008).

    Article  CAS  PubMed  Google Scholar 

  123. Thameem, F. et al. The Gly(972)Arg variant of human IRS1 gene is associated with variation in glomerular filtration rate likely through impaired insulin receptor signaling. Diabetes 61, 2385–2393 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  124. Yano, N. et al. In vitro silencing of the insulin receptor attenuates cellular accumulation of fibronectin in renal mesangial cells. Cell Commun. Signal. 10, 29 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  125. Isshiki, K. et al. Insulin regulates SOCS2 expression and the mitogenic effect of IGF-1 in mesangial cells. Kidney Int. 74, 1434–1443 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  126. Kong, Y. L. et al. Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway. Acta Pharmacol. Sin. 37, 217–227 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  127. Weigert, C. et al. Evidence for a novel TGF-beta1-independent mechanism of fibronectin production in mesangial cells overexpressing glucose transporters. Diabetes 52, 527–535 (2003).

    Article  CAS  PubMed  Google Scholar 

  128. Coward, R. J. et al. Nephrin is critical for the action of insulin on human glomerular podocytes. Diabetes 56, 1127–1135 (2007).

    Article  CAS  PubMed  Google Scholar 

  129. Kim, E. Y., Anderson, M. & Dryer, S. E. Insulin increases surface expression of TRPC6 channels in podocytes: role of NADPH oxidases and reactive oxygen species. Am. J. Physiol. Renal Physiol. 302, F298–F307 (2012).

    Article  CAS  PubMed  Google Scholar 

  130. Kim, E. Y. & Dryer, S. E. Effects of insulin and high glucose on mobilization of slo1 BKCa channels in podocytes. J. Cell. Physiol. 226, 2307–2315 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  131. Tejada, T. et al. Failure to phosphorylate AKT in podocytes from mice with early diabetic nephropathy promotes cell death. Kidney Int. 73, 1385–1393 (2008).

    Article  CAS  PubMed  Google Scholar 

  132. Welsh, G. I. et al. Insulin signaling to the glomerular podocyte is critical for normal kidney function. Cell Metab. 12, 329–340 (2010). This study shows podocyte loss upon disruption of insulin signalling, highlighting the essential role of insulin in podocyte health and viability.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  133. Madhusudhan, T. et al. Defective podocyte insulin signalling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy. Nat. Commun. 6, 6496 (2015).

    Article  CAS  PubMed  Google Scholar 

  134. Baum, M. Insulin stimulates volume absorption in the rabbit proximal convoluted tubule. J. Clin. Invest. 79, 1104–1109 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  135. Takahashi, N., Ito, O. & Abe, K. Tubular effects of insulin. Hypertens. Res. 19 (Suppl. 1), S41–S45 (1996).

    Article  CAS  PubMed  Google Scholar 

  136. DeFronzo, R. A., Goldberg, M. & Agus, Z. S. The effects of glucose and insulin on renal electrolyte transport. J. Clin. Invest. 58, 83–90 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  137. Nizet, A., Lefebvre, P. & Crabbe, J. Control by insulin of sodium potassium and water excretion by the isolated dog kidney. Pflugers Arch. 323, 11–20 (1971).

    Article  CAS  PubMed  Google Scholar 

  138. Brands, M. W., Hildebrandt, D. A., Mizelle, H. L. & Hall, J. E. Sustained hyperinsulinemia increases arterial pressure in conscious rats. Am. J. Physiol. 260, R764–R768 (1991).

    CAS  PubMed  Google Scholar 

  139. Brands, M. W. & Manhiani, M. M. Sodium-retaining effect of insulin in diabetes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 303, R1101–R1109 (2012). This review unravels the controversy regarding the role of insulin in sodium transport in vivo.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  140. Manhiani, M. M., Cormican, M. T. & Brands, M. W. Chronic sodium-retaining action of insulin in diabetic dogs. Am. J. Physiol. Renal Physiol. 300, F957–F965 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  141. Blazer-Yost, B. L., Esterman, M. A. & Vlahos, C. J. Insulin-stimulated trafficking of ENaC in renal cells requires PI 3-kinase activity. Am. J. Physiol. Cell Physiol. 284, C1645–C1653 (2003).

    Article  CAS  PubMed  Google Scholar 

  142. Lang, F., Artunc, F. & Vallon, V. The physiological impact of the serum and glucocorticoid-inducible kinase SGK1. Curr. Opin. Nephrol. Hypertens. 18, 439–448 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  143. Lang, F. et al. Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy. Proc. Natl Acad. Sci. USA 97, 8157–8162 (2000).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  144. Tiwari, S. et al. Impaired sodium excretion and increased blood pressure in mice with targeted deletion of renal epithelial insulin receptor. Proc. Natl Acad. Sci. USA 105, 6469–6474 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  145. Li, L., Garikepati, R. M., Tsukerman, S., Tiwari, S. & Ecelbarger, C. M. Salt sensitivity of nitric oxide generation and blood pressure in mice with targeted knockout of the insulin receptor from the renal tubule. Am. J. Physiol. Regul. Integr. Comp. Physiol. 303, R505–R512 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  146. Li, L. et al. Reduced ENaC activity and blood pressure in mice with genetic knockout of the insulin receptor in the renal collecting duct. Am. J. Physiol. Renal Physiol. 304, F279–F288 (2013).

    Article  CAS  PubMed  Google Scholar 

  147. Pavlov, T. S. et al. Regulation of ENaC in mice lacking renal insulin receptors in the collecting duct. FASEB J. 27, 2723–2732 (2013). This paper shows decreased ENaC activity in mice that lack the insulin receptor in the AQP2-expressing distal tubule.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  148. Stumvoll, M., Meyer, C., Mitrakou, A. & Gerich, J. E. Important role of the kidney in human carbohydrate metabolism. Med. Hypotheses 52, 363–366 (1999).

    Article  CAS  PubMed  Google Scholar 

  149. Tiwari, S. et al. Deletion of the insulin receptor in the proximal tubule promotes hyperglycemia. J. Am. Soc. Nephrol. 24, 1209–1214 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  150. Eid, A. et al. Intrinsic gluconeogenesis is enhanced in renal proximal tubules of Zucker diabetic fatty rats. J. Am. Soc. Nephrol. 17, 398–405 (2006).

    Article  CAS  PubMed  Google Scholar 

  151. Ghezzi, C. & Wright, E. M. Regulation of the human Na+-dependent glucose cotransporter hSGLT2. Am. J. Physiol. Cell Physiol. 303, C348–C354 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  152. Vallon, V. et al. Knockout of Na-glucose transporter SGLT2 attenuates hyperglycemia and glomerular hyperfiltration but not kidney growth or injury in diabetes mellitus. Am. J. Physiol. Renal Physiol. 304, F156–F167 (2013).

    Article  CAS  PubMed  Google Scholar 

  153. Wilding, J. P. The role of the kidneys in glucose homeostasis in type 2 diabetes: clinical implications and therapeutic significance through sodium glucose co-transporter 2 inhibitors. Metabolism 63, 1228–1237 (2014).

    Article  CAS  PubMed  Google Scholar 

  154. Accili, D. et al. Early neonatal death in mice homozygous for a null allele of the insulin receptor gene. Nat. Genet. 12, 106–109 (1996).

    Article  CAS  PubMed  Google Scholar 

  155. Joshi, R. L. et al. Targeted disruption of the insulin receptor gene in the mouse results in neonatal lethality. EMBO j 15, 1542–1547 (1996).

    Article  CAS  Google Scholar 

  156. Accili, D. Insulin Receptor Knock-Out Mice. Trends Endocrinol. Metabolism 8, 101–104 (1997).

    Article  CAS  Google Scholar 

  157. Brüning, J. C. et al. A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Mol. Cell 2, 559–569 (1998).

    Article  PubMed  Google Scholar 

  158. Michael, M. D. et al. Loss of insulin signaling in hepatocytes leads to severe insulin resistance and progressive hepatic dysfunction. Mol. Cell 6, 87–97 (2000).

    Article  CAS  PubMed  Google Scholar 

  159. Mima, A. et al. Glomerular-specific protein kinase C-beta-induced insulin receptor substrate-1 dysfunction and insulin resistance in rat models of diabetes and obesity. Kidney Int. 79, 883–896 (2011). This study investigates insulin signalling in the glomeruli and renal tubules and shows that insulin-resistance occurs only in the glomeruli.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  160. Rocchini, A. P. et al. Insulin and renal sodium retention in obese adolescents. Hypertension 14, 367–374 (1989).

    Article  CAS  PubMed  Google Scholar 

  161. Skott, P. et al. Effect of insulin on renal sodium handling in hyperinsulinaemic type 2 (non-insulin-dependent) diabetic patients with peripheral insulin resistance. Diabetologia 34, 275–281 (1991).

    Article  CAS  PubMed  Google Scholar 

  162. Nakamura, M. et al. Stimulatory effect of insulin on renal proximal tubule sodium transport is preserved in type 2 diabetes with nephropathy. Biochem. Biophys. Res. Commun. 461, 154–158 (2015).

    Article  CAS  PubMed  Google Scholar 

  163. Nakamura, M. et al. Preserved Na/HCO3 cotransporter sensitivity to insulin may promote hypertension in metabolic syndrome. Kidney Int. 87, 535–542 (2015).

    Article  CAS  PubMed  Google Scholar 

  164. Grahammer, F. et al. mTORC2 critically regulates renal potassium handling. J. Clin. Invest. 126, 1773–1782 (2016). This study proves that mTORC2 is the hydrophobic motif kinase of SGK1.

    Article  PubMed  PubMed Central  Google Scholar 

  165. Gerich, J. E. Role of the kidney in normal glucose homeostasis and in the hyperglycaemia of diabetes mellitus: therapeutic implications. Diabet. Med. 27, 136–142 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  166. Meyer, C. et al. Abnormal renal and hepatic glucose metabolism in type 2 diabetes mellitus. J. Clin. Invest. 102, 619–624 (1998). This study demonstrates insulin resistance of renal gluconeogenesis in patients with type 2 diabetes.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  167. Zheng, Y. et al. Roles of insulin receptor substrates in insulin-induced stimulation of renal proximal bicarbonate absorption. J. Am. Soc. Nephrol. 16, 2288–2295 (2005).

    Article  CAS  PubMed  Google Scholar 

  168. Schafer, S. et al. Lifestyle intervention in individuals with normal versus impaired glucose tolerance. Eur. J. Clin. Invest. 37, 535–543 (2007).

    Article  CAS  PubMed  Google Scholar 

  169. Machann, J. et al. Follow-up whole-body assessment of adipose tissue compartments during a lifestyle intervention in a large cohort at increased risk for type 2 diabetes. Radiology 257, 353–363 (2010).

    Article  PubMed  Google Scholar 

  170. Stefan, N. et al. A high-risk phenotype associates with reduced improvement in glycaemia during a lifestyle intervention in prediabetes. Diabetologia 58, 2877–2884 (2015).

    Article  CAS  PubMed  Google Scholar 

  171. Cohen, J. B. & Cohen, D. L. Cardiovascular and renal effects of weight reduction in obesity and the metabolic syndrome. Curr. Hypertens. Rep. 17, 34 (2015).

    Article  CAS  PubMed  Google Scholar 

  172. Rocchini, A. P. et al. The effect of weight loss on the sensitivity of blood pressure to sodium in obese adolescents. N. Engl. J. Med. 321, 580–585 (1989).

    Article  CAS  PubMed  Google Scholar 

  173. Lavrencic, A., Salobir, B. G. & Keber, I. Physical training improves flow-mediated dilation in patients with the polymetabolic syndrome. Arterioscler Thromb. Vasc. Biol. 20, 551–555 (2000).

    Article  CAS  PubMed  Google Scholar 

  174. Vinet, A. et al. Impact of a lifestyle program on vascular insulin resistance in metabolic syndrome subjects: the RESOLVE study. J. Clin. Endocrinol. Metab. 100, 442–450 (2015).

    Article  CAS  PubMed  Google Scholar 

  175. Thamer, C. et al. High visceral fat mass and high liver fat are associated with resistance to lifestyle intervention. Obesity (Silver Spring) 15, 531–538 (2007).

    Article  Google Scholar 

  176. Fenske, W. et al. Obesity-related cardiorenal disease: the benefits of bariatric surgery. Nat. Rev. Nephrol. 9, 539–551 (2013).

    Article  PubMed  Google Scholar 

  177. American Diabetes Association. Approaches to glycemic treatment. Diabetes Care 39 (Suppl. 1), S52–S59 (2016).

  178. Sarafidis, P. A. & Lasaridis, A. N. Actions of peroxisome proliferator–activated receptors–γ agonists explaining a possible blood pressure–lowering effect. Am. J. Hypertension 19, 646–653 (2006).

    Article  CAS  Google Scholar 

  179. Sarafidis, P. A., Stafylas, P. C., Georgianos, P. I., Saratzis, A. N. & Lasaridis, A. N. Effect of thiazolidinediones on albuminuria and proteinuria in diabetes: a meta-analysis. Am. J. Kidney Dis. 55, 835–847 (2010).

    Article  CAS  PubMed  Google Scholar 

  180. Dagenais, G. R. et al. Effects of ramipril and rosiglitazone on cardiovascular and renal outcomes in people with impaired glucose tolerance or impaired fasting glucose: results of the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) trial. Diabetes Care 31, 1007–1014 (2008).

    Article  CAS  PubMed  Google Scholar 

  181. Artunc, F. et al. Lack of the serum and glucocorticoid-inducible kinase SGK1 attenuates the volume retention after treatment with the PPARgamma agonist pioglitazone. Pflugers Arch. 456, 425–436 (2008).

    Article  CAS  PubMed  Google Scholar 

  182. Ochi, A. et al. Direct inhibitory effects of pioglitazone on hepatic fetuin-A expression. PLoS ONE 9, e88704 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  183. Mori, K. et al. Effects of pioglitazone on serum fetuin-A levels in patients with type 2 diabetes mellitus. Metabolism 57, 1248–1252 (2008).

    Article  CAS  PubMed  Google Scholar 

  184. Poitout, V. & Robertson, R. P. Glucolipotoxicity: fuel excess and beta-cell dysfunction. Endocr. Rev. 29, 351–366 (2008).

    Article  CAS  PubMed  Google Scholar 

  185. Bensellam, M., Laybutt, D. R. & Jonas, J. C. The molecular mechanisms of pancreatic beta-cell glucotoxicity: recent findings and future research directions. Mol. Cell Endocrinol. 364, 1–27 (2012).

    Article  CAS  PubMed  Google Scholar 

  186. Kaul, K., Apostolopoulou, M. & Roden, M. Insulin resistance in type 1 diabetes mellitus. Metabolism 64, 1629–1639 (2015).

    Article  CAS  PubMed  Google Scholar 

  187. Hanefeld, M., Monnier, L., Schnell, O. & Owens, D. Early treatment with basal insulin glargine in people with type 2 diabetes: lessons from ORIGIN and other cardiovascular trials. Diabetes Ther. 7, 187–201 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  188. Gerstein, H. C. et al. Basal insulin and cardiovascular and other outcomes in dysglycemia. N. Engl. J. Med. 367, 319–328 (2012).

    Article  CAS  PubMed  Google Scholar 

  189. Gilbert, R. E. et al. Basal insulin glargine and microvascular outcomes in dysglycaemic individuals: results of the Outcome Reduction with an Initial Glargine Intervention (ORIGIN) trial. Diabetologia 57, 1325–1331 (2014).

    Article  CAS  PubMed  Google Scholar 

  190. Ferrannini, E. et al. Metabolic response to sodium-glucose cotransporter 2 inhibition in type 2 diabetic patients. J. Clin. Invest. 124, 499–508 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  191. Merovci, A. et al. Dapagliflozin improves muscle insulin sensitivity but enhances endogenous glucose production. J. Clin. Invest. 124, 509–514 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  192. Zinman, B. et al. Empagliflozin, cardiovascular outcomes, and mortality in type 2 diabetes. N. Engl. J. Med. 373, 2117–2128 (2015).

    Article  CAS  PubMed  Google Scholar 

  193. Wanner, C. et al. Empagliflozin and progression of kidney disease in type 2 diabetes. N. Engl. J. Med. 375, 323–334 (2016).

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We acknowledge the meticulous work of Marketa Kovarova (Department of Internal Medicine IV, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University Hospital Tübingen, Germany) in designing the figures. The authors' work is funded by a grant from the German Federal Ministry of Education and Research to the German Centre for Diabetes Research (DZD), München-Neuherberg, Germany.

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All authors researched the data for the article, discussed the content, wrote the article and reviewed and/or edited the manuscript before submission.

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Correspondence to Hans-Ulrich Häring.

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PowerPoint slides

Glossary

Impaired glucose tolerance

Defined as a plasma glucose concentration of 140–200 mg/dl (7.77–11.1 mmol/l) measured 2 h after an oral glucose load of 75 g.

Visceral obesity

Increased waist circumference as a result of an accumulation of fat in the intra-abdominal compartments, such as the omentum majus.

Hepatokines

Factors that are secreted from the liver and act on other tissues.

Hyperinsulinaemic–euglycaemic clamp

Test used to quantify insulin resistance on a whole-body level. Continuous insulin infusion is used to maintain plasma insulin levels, whilst variable glucose infusion is used to maintain plasma glucose concentration at basal levels. When a stable plasma glucose concentration is achieved, the rate of glucose infusion is equal to the rate of glucose uptake by all of the body tissues.

Renal resistive index

A measure of intrarenal vascular resistance.

Kimmelstiel–Wilson lesions

The typical histopathological hallmark of diabetic nephropathy, which is characterized by nodular glomerulosclerosis.

Impaired fasting glucose

Defined as a plasma glucose concentration of 110–126 mg/dl (6.11–6.99 mmol/l) in the fasting state.

Oral glucose tolerance test

Test used to screen for disturbances in glucose metabolism and insulin resistance.

Liver steatosis

Accumulation of excess fat in the liver.

Homeostasis model assessment of insulin resistance

A simple quantitative measure of insulin resistance calculated from the plasma fasting glucose level and insulin concentration.

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Artunc, F., Schleicher, E., Weigert, C. et al. The impact of insulin resistance on the kidney and vasculature. Nat Rev Nephrol 12, 721–737 (2016). https://doi.org/10.1038/nrneph.2016.145

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