Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Genome watch

Singled out


This month's Genome Watch reviews a recent article that demonstrates the use of single-cell genomics as a means of characterizing uncultivated microorganisms.


The vast majority of microorganisms are not amenable to cultivation in the laboratory, and as a result we have a substantially incomplete and skewed knowledge of microbial life. Insights from small-subunit ribosomal RNA gene surveys and, more recently, metagenomic analyses have revealed the enormous extent of unknown microbial species and gene diversity. However, these techniques do not readily permit the study of direct interactions between organisms in situ and cannot define the coding potential of individual cells within a given environment. A complementary approach is the burgeoning field of single-cell genomics (see Ref. 1 for a brief overview), in which individual cells are isolated from environmental samples and their genomic DNA is amplified and sequenced.

A recent study by Yoon et al.2 used single-cell genomics in an attempt to characterize novel heterotrophic protists from a 50 ml sample of sea water taken off the coast of Maine, USA. To this end, the authors used fluorescence-activated cell sorting to specifically separate cells that lacked chlorophyll fluorescence, and amplified the genomes of these cells using multiple displacement amplification (MDA). They then determined the taxonomies of these isolates by sequencing their 18S rRNA genes. This revealed that six out of the 35 single-cell amplified genomes (SAGs) were derived from picobiliphytes, a distinct group of marine plankton that was initially identified from environmental 18S rRNA gene surveys and which currently has no cultivated members3. Previous work had indicated that picobiliphytes could have a photosynthetic lifestyle, owing to the apparent presence of plastid-like organelles with orange fluorescence indicative of phycobilin proteins3. The authors therefore shotgun sequenced three picobiliphyte SAGs using the 454 FLX Titanium platform in the hope that the genomes would reveal whether these cells were heterotrophic or photosynthetic. This generated 90 Mb of individual reads and 5 Mb of assembled contigs per SAG. The authors found no genomic evidence for the presence of plastids in any of the three SAGs, suggesting that these picobiliphytes are in fact heterotrophs. To rule out the possibility that plastid-related genes were simply missed owing to MDA bias, deep Illumina sequencing was carried out on two of the SAGs, but such genes were not detected. By contrast, in an experimental control the authors were able to recover more than 50% of the plastid-related genes from MDA-amplified DNA from the photosynthetic amoeba Paulinella chromatophora.

The SAGs also provided fascinating insights into interactions between the picobiliphytes and other environmental microorganisms. One of the SAGs contained a large number of sequence reads belonging to a previously uncharacterized single-stranded DNA virus, indicating that the authors had captured a cell in the midst of an infection by the viral invader. The assembled genome of this virus was compared with metagenomic data from the Global Ocean Survey4, and this revealed that related viruses may be abundant in the ocean. The other two picobiliphyte cells did not appear to contain the single-stranded DNA virus. Instead, non-eukaryotic sequences seemed to correspond to DNA from marine Bacteroidetes, Proteobacteria and large DNA viruses. Although the authors concede that these sequences may result from contamination or attachment of foreign DNA to the surface of the isolated cells, they speculate that these microorganisms may be a food source for the picobiliphytes.

Although the single-cell approach suffers from complications such as amplification bias and contamination issues, this work hints at the future possibilities for single-cell genomics as the technology improves and the cost of sequencing continues to fall. We are approaching the era in which we can at last begin to understand the part that any cell plays in a given environment, without the requirement for prior cultivation.


  1. 1

    Walker, A. & Parkhill, J. Single-cell genomics. Nature Rev. Microbiol. 6, 176–177 (2008).

    CAS  Article  Google Scholar 

  2. 2

    Yoon, H. S. et al. Single-cell genomics reveals organismal interactions in uncultivated marine protists. Science 332, 714–717 (2011).

    CAS  Article  Google Scholar 

  3. 3

    Not, F. et al. Picobiliphytes: a marine picoplanktonic algal group with unknown affinities to other eukaryotes. Science 315, 253–255 (2007).

    CAS  Article  PubMed  Google Scholar 

  4. 4

    Williamson, S. J. et al. The Sorcerer II Global Ocean Sampling Expedition: metagenomic characterization of viruses within aquatic microbial samples. PLoS ONE 3, e1456 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information



Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Walker, A. Singled out. Nat Rev Microbiol 9, 485 (2011).

Download citation


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing