Malaria is a major health problem, mainly in sub-Saharan Africa and in some parts of Asia and south America. Each year there are about 600 million new clinical cases and at least one million individuals, mostly children, die from malaria; in other words there is a death caused by malaria every 30 seconds.
Successful malaria transmission to the mammalian host is established through sporozoite infection of the liver. The importance of the liver stages, owing to its status as an obligatory step in infection, makes this stage an attractive target for both drug and vaccine development.
In recent years, our understanding of Plasmodium sporozoite biology, the interactions of sporozoites with different host cells and their establishment in the liver, has increased through the implementation of new technologies that allow researchers to follow the progress of the parasites from the site of injection to the liver.
Plasmodium sporozoites are deposited in the skin of their vertebrate host by the bite of an infected female Anopheles mosquito. A proportion of these parasites are drained to the lymph nodes, where they are trapped, whereas the majority find a blood vessel and travel in the peripheral blood circulation until they reach the liver sinusoids.
Once arrested in the liver sinusoids, the sporozoites cross the sinusoidal wall and migrate through several hepatocytes by breaching their plasma membrane, before they infect a final hepatocyte with the formation of a parasitophorous vacuole. The intrahepatic form of the parasite grows and multiplies in this vacuole, producing thousands of merozoites per sporozoite.
This Review summarizes our current knowledge on this stage of the Plasmodium life cycle.
Plasmodium sporozoites are deposited in the skin of their vertebrate hosts through the bite of an infected female Anopheles mosquito. Most of these parasites find a blood vessel and travel in the peripheral blood circulation until they reach the liver sinusoids. Once there, the sporozoites cross the sinusoidal wall and migrate through several hepatocytes before they infect a final hepatocyte, with the formation of a parasitophorous vacuole, in which the intrahepatic form of the parasite grows and multiplies. During this period, each sporozoite generates thousands of merozoites. As the development of Plasmodium sporozoites inside hepatocytes is an obligatory step before the onset of disease, understanding the parasite's requirements during this period is crucial for the development of any form of early intervention. This Review summarizes our current knowledge on this stage of the Plasmodium life cycle.
Despite the fact that several countries were able to eradicate malaria during the past century, it remains one of the most prevalent infectious diseases worldwide. Forty percent of the world's population is at risk of infection, and 500 million people become infected and up to 3 million children die every year1. Malaria also has devastating economic consequences as it drains as much as 2% of the gross domestic product of countries in sub-Saharan Africa.
Malaria infection is initiated when Plasmodium sporozoites enter the mammalian host through the bite of an infected female Anopheles mosquito. During a blood meal, an average of 15–123 sporozoites have been reported to be deposited under the skin of the host, which migrate to the liver, where they infect hepatocytes and begin to develop into merozoites2,3,4,5. Between 2 and 16 days later, depending on the Plasmodium species, thousands of merozoites per invading sporozoite are released into the bloodstream6,7,8. Each merozoite will invade an erythrocyte, initiating a replication cycle that ends with the release of new merozoites from the mature infected erythrocyte (schizont), which go on to infect other erythrocytes. Malaria-associated pathology only occurs during the blood stage of infection. The Plasmodium life cycle continues when some merozoites develop into the sexual parasite stages, the male and female gametocytes, which can be taken up by mosquitoes during blood meals. Gametocytes undergo fertilization and maturation in the mosquito midgut, forming an infective ookinete form that migrates through the mosquito midgut into the hemocele, developing into the oocyst in which sporozoites are formed. When fully matured, the oocysts burst and release sporozoites, which migrate into the mosquito's salivary glands, ready for the next transmission step.
An obligatory step during infection is the establishment and full development of Plasmodium sporozoites inside hepatocytes, which, although symptomatically silent, gives rise to thousands of merozoites in each hepatocyte. Similar to many of the invasive stages of other parasites in the phylum Apicomplexa, Plasmodium sporozoites have two interconnected characteristics that are crucial for the completion of their life cycle: gliding motility9 and the capacity to migrate through host cells10. The infection of host cells by Plasmodium sporozoites requires sporozoite motility11 and presumably involves invagination of the plasma membrane, with the formation of a parasitophorous vacuole around the parasite12. Plasmodium sporozoites can also enter and exit host cells by breaching the plasma membrane, a process that requires gliding motility and is used to migrate through host cells and tissues10.
Four different species of Plasmodium can cause malaria in humans: Plasmodium falciparum , Plasmodium vivax , Plasmodium ovale and Plasmodium malariae. P. falciparum is by far the deadliest of the four and is responsible for most of the mortality and morbidity associated with malaria. It is worth mentioning that P. vivax and P. ovale can exist in dormant forms in the liver, called hypnozoites. The latent nature of this stage is responsible for the relapses that can occur when malaria is caused by either of these two species13. A significant amount of research involving the Plasmodium pre-erythrocytic stages has made use of Plasmodium spp. that infect rodents, specifically Plasmodium berghei and Plasmodium yoelii . These two species present differences in infectivity, which depend not only on the species but also on the clone and the genetic background of the rodent host14,15,16. P. berghei remains the most widely used rodent parasite because the technologies to enable transfection were developed earlier for P. berghei17 than for P. yoelii18. These differences should always be taken into account when interpreting results.
From the skin to the liver
When an infected mosquito bites a mammalian host, it probes for a blood source under the skin. During this process, the mosquito injects saliva containing vasodilators and anti-coagulants to facilitate the ingestion of blood19,20. If the mosquito is infected and contains Plasmodium sporozoites in its salivary glands, a small number of these are deposited in the skin during the blood meal3 (Fig. 1). Most sporozoites are injected into the dermis and not directly into the circulation2,21,22. After injection into the skin, the sporozoites move through the dermis until they come into contact with a blood vessel and move into the circulatory system, which allows them to travel to the liver2,22. A proposed alternative route for the sporozoite journey to the liver is through the lymphatic system, possibly inside leukocytes23,24. Intravital microscopy work22 using the P. berghei rodent model of malaria showed that sporozoites deposited into avascular dermal tissue use gliding motility to migrate through the skin and into dermal vessels, a process that can take over 30 minutes. It has been proposed that a sporozoite surface phospholipase (PbPL) is required to breach host cell membranes during migration in the skin, as parasites that are deficient in PbPL are impaired in their ability to cross epithelial cell monolayers, and their infectivity is greatly decreased when they are transmitted by mosquito bite25. During migration in the skin the parasite might be vulnerable to antibodies against Plasmodium surface proteins, which might function as the first line of the host's acquired immune response against the parasite22.
Recent intravital microscopy observations showed that a significant proportion of mosquito-injected sporozoites remain in the dermis after gliding has stopped2. Of those that leave the area of the bite within 1 hour of injection, approximately 70% enter the blood vessels and the remaining 30% invade the lymphatic vessels. Most of those entering the lymphatic vessels do not reach the circulatory system, as had been previously assumed. Instead, they are trapped in the lymph nodes, where most are phagocytosed by dendritic cells. Some of these lymphatic sporozoites have been found to partially develop into small-sized exoerythrocytic forms, similar to the Plasmodium stage that develops inside hepatocytes, before eventually being degraded2. Whether the presence of parasites in an important organ of the immune system has any influence on the development of the anti-malarial immune response remains to be elucidated.
Arrest in the liver
Once inside the circulatory system, sporozoites rapidly reach the liver sinusoids (Fig. 2). Specific targeting of the liver by sporozoites is efficient, as they are found in hepatocytes as early as 2 minutes after intravenous injection into mice26. The selectivity of this process suggests that it involves specific interactions between parasite-encoded surface protein(s) and host molecule(s).
Both host and parasite molecules involved in sporozoite arrest have been extensively studied. A major Plasmodium sporozoite surface protein, the circumsporozoite protein (CSP), seems to have a crucial role in these processes by interacting with the heparan sulphate proteoglycans (HSPGs) of the liver cells. Initially, it was shown that recombinant CSP binds specifically to hepatocyte microvilli within the space of Disse, a space that separates the sinusoidal endothelium from hepatocytes27,28,29. Two domains in CSP, a thrombospondin-like cell-adhesive region II-plus at the carboxyl terminus and a positively charged motif upstream from conserved region I, seem crucial for this binding28,30,31.
In the host, the HSPGs on the surface of hepatocytes are considered the main receptors for Plasmodium attachment in the liver sinusoids32,33. As liver endothelial cells have fenestrations, it has been proposed that hepatocyte or extracellular matrix HSPGs extending through endothelial fenestrations to the sinusoidal lumen can interact with CSP, leading to sporozoite sequestration32. Although HSPGs are present in most tissues, those associated with cells in the liver have a significantly greater degree of sulphation than those present in other tissues34, which might account for the selective recognition of recombinant CSP and Plasmodium sporozoites in the liver. In particular, sulphation of the glycosaminoglycan chains at both the N- and O-positions is required for sporozoite adhesion to cells35.
More recently, the proteoglycan species produced by different liver cell types have been characterized. CSP and thrombospondin-related anonymous protein (TRAP) recognize distinct cell-type-specific surface proteoglycans, not only on hepatocytes, but also on Kupffer cells and stellate cells. Moreover, because stellate cells synthesize eight times more sulphated proteoglycans than hepatocytes and incorporate twice the amount of sulphate into heparan sulphate, matrix proteoglycans that are produced by stellate cells and protrude through the endothelial fenestrations have been suggested to mediate the initial arrest of Plasmodium sporozoites in the liver sinusoids36,37.
After being sequestered in the sinusoids, sporozoites must reach and invade the hepatocytes (Fig. 2). They encounter two different cell types on the way: endothelial and Kupffer cells. Although liver endothelial cells have fenestrations, these are too small (about one tenth of the diameter of a sporozoite) to allow sporozoite passage27. As sporozoites can migrate through all nucleated cell types tested so far by disrupting their plasma membrane (M.M.M., unpublished results), it is plausible that sporozoites could traverse either endothelial or Kupffer cells. However, there is mounting evidence to indicate that sporozoites cross the sinusoidal layer primarily through Kupffer cells38.
First, in vitro, in co-cultures of Kupffer and primary liver endothelial cells, sporozoites were found to preferentially invade the former39. Interestingly, invasion of Kupffer cells by sporozoites is reported to occur with the formation of a parasitophorous vacuole. However, because this vacuole does not fuse with lysosomes the authors suggested that this process does not involve phagocytosis12,39. Therefore, according to these results, crossing the sinusoidal layer occurs via Kupffer cells, not by traversing the cell by disrupting the plasma membrane but by the formation of a vacuole. Moreover, using intravital microscopy, all sporozoites arrested in the liver sinusoids were found to move towards the hepatocytes through Kupffer cells at a lower speed than the speed measured when sporozoites traverse cells using plasma-membrane disruption40. However, it has recently been reported that P. berghei parasites deficient in the SPECT (sporozoite microneme protein essential for cell traversal) and SPECT2 (also called Plasmodium perforin-like protein 1, PPLP1) proteins, which confers a defect in the ability to traverse cells, have low infectivity in vivo41,42. These results seem to indicate that the crossing of the sinusoidal layer can also occur by the disruption of cell plasma membranes. As the removal of Kupffer cells increased the infectivity of SPECT-deficient sporozoites, the authors also interpreted these results as a confirmation of the essential role of Kupffer cells in sporozoite infection of the liver. However, the treatment to deplete Kupffer cells might have left small discontinuities in the sinusoidal endothelial layer that would allow parasites to reach hepatocytes directly. Therefore, it is not clear from these results whether during normal infection, in the absence of discontinuities, Kupffer cells are the cells that are preferentially traversed. It is likely that sporozoites can use more than one cellular pathway to cross the sinusoidal endothelial layer, as a proportion of cell-traversal-deficient parasites can still infect the liver and propagate in mice41,42.
After crossing the space of Disse in the liver, sporozoites migrate through several hepatocytes before invading a final hepatocyte in which a parasitophorous vacuole is formed10,43 (Fig. 3). Using a cell-wounding assay, it has been shown both in vitro and in vivo that during migration through cells, Plasmodium spp. sporozoites breach the plasma membranes of several hepatocytes, which can rapidly be repaired10. Recently, sporozoite migration in the liver was confirmed by intravital microscopy40.
Breaching of the cell membranes by the Plasmodium parasite is likely to involve specific lipases, proteases and pore-forming proteins. Four distinct P. berghei proteins have been shown to have important roles during cell traversal: SPECT, SPECT2, cell traversal protein for ookinete and sporozoite (CelTOS) and the phospholipase PbPL25,41,42,44. At least two of these proteins, SPECT2 and PbPL, seem to be involved in pore formation25,41, whereas CelTOS has been proposed to be required for movement through the host-cell cytosol44.
It has been shown that migration through cells leads to regulated exocytosis of sporozoite secretory organelles, resulting in the exposure of membrane proteins from these organelles, such as TRAP, on the apical end of the parasite45. TRAP is normally visualized as a relatively faint staining distributed along the body of the parasite but, after incubation with host cells, high concentrations of TRAP are observed forming an apical 'cap' on the sporozoite surface46. This probably contributes to the tight interactions observed between the sporozoite and the hepatocyte cell membrane, which are believed to drive internalization47. Elevated cytosolic concentrations of Ca2+ in sporozoites induce exocytosis45,46, suggesting that signalling cascades might be activated in the parasite during migration through cells. Another example of the ability of sporozoites to respond to host cells is provided by the cleavage of the surface sporozoite protein CSP, which is induced by contact with hepatocytes and is required for hepatocyte infection in vitro and in vivo48.
It is generally accepted that sporozoites use gliding motility and their ability to migrate through cells in their journey from the skin into the liver, and only commit to the final infection after contact with hepatocytes49,50. What triggers the switch from sporozoite migration through cells to infection is still unknown. Disruption of the spect gene was shown to impair the ability of sporozoites to migrate through hepatocytes42, but spect-deficient sporozoites can still infect hepatocytes42. Other genes were identified, the disruption of which led to a similar phenotype41,44. Therefore, the authors suggested that, although migration through cells is necessary to reach the hepatic parenchyma, migration through hepatocytes is not necessary for infection42. So, some data show that migration through host cells activates exocytosis, enhancing infection and that inhibition of exocytosis inhibits infection45, whereas other data show that spect-deficient parasites, which are unable to migrate through cells, can infect hepatocytes in vitro42. These apparently conflicting results need further attention to be reconciled.
As sporozoite migration through cells occurs by the breaching of the plasma membrane, this leads to liver injury at the microenvironment level10,40. We have shown that host cell wounding by sporozoite migration induces the secretion of infection-susceptibility-inducing factors, which render hepatocytes more susceptible to infection51. One such factor is hepatocyte growth factor (HGF), which, by activating its receptor MET, leads to an increase in infection, not by functioning as a binding site for Plasmodium sporozoites, but as a mediator of signals that make the host cell more permissive to Plasmodium infection51. Again, however, the spect mutant phenotype questions the requirement of host factors released by traversed cells for infection.
Following migration through cells, Plasmodium sporozoites engage in a final invasion, with the formation of a parasitophorous vacuole. Both TRAP and CSP interact with hepatocytes. Whereas CSP seems to have an active role in sporozoite attachment rather than internalization35, TRAP seems to contribute to sporozoite internalization and not attachment52. Recently, a protein termed apical membrane antigen 1 (AMA-1) was shown to be expressed in sporozoites and was implicated in the invasion of hepatocytes by P. falciparum parasites in vitro53. Two other P. berghei proteins, Pb36p and Pb36, which are specifically produced in liver-infective sporozoites and belong to the Plasmodium 6-Cys domain protein family, are also necessary for sporozoites to recognize hepatocytes and commit to infection54.
HSPGs on the surface of hepatocytes seem to have an important role in binding and internalizing sporozoites. They interact with both CSP and TRAP, as mentioned earlier. The extracellular region of TRAP has also been shown to interact with the serum glycoprotein fetuin A on hepatocyte membranes, an interaction that enhances the parasite's ability to invade hepatocytes55. Another host molecule that seems to interact directly or indirectly with sporozoites is the tetraspanin CD81, a membrane protein that is expressed on the surface of hepatocytes and is a putative receptor for hepatitis C virus56. CD81 seems to have an essential role in the invasion of mouse hepatocytes by P. yoelii and human hepatocytes by P. falciparum43, which it has been proposed is linked to the fact that CD81 is localized in tetraspanin-enriched microdomains57. By contrast, infection of HepG2 cells by P. berghei seems to be independent of CD81 expression43. Despite efforts to identify a Plasmodium molecule that interacts with CD81, no proof of a direct interaction has been obtained43. Therefore, the authors proposed that CD81 might regulate the activity of another host molecule that has an essential role in sporozoite invasion, which is in agreement with the reported ability of tetraspanins to associate with, and regulate the function of, molecular partners43.
In the search for the host receptors that are required for invasion, several host molecules have been shown to not be essential for sporozoite infection. Mice that are deficient in intercellular adhesion molecule 1 (ICAM-1) and ICAM-2 showed similar levels of infection with P. yoelii sporozoites as wild-type mice58. Similar results were also obtained for mice deficient in CD36 (a scavenger receptor expressed by many cell types including Kupffer and endothelial cells) and syndecan-1 (a major transmembrane HSPG expressed by many cell types, including the sinusoidal, basolateral side of hepatocytes)59,60. However, given that CSP binds to the glycosaminoglycan portion, and not to the core protein, of the various syndecans; that cells typically express several syndecan species; and that a given type of cell normally links the same species of glycosaminoglycan chains to all of the syndecans it produces, the lack of effect of deleting the core protein of one of the syndecans, syndecan-1, does not necessarily mean that these molecules are not involved in sporozoite infection. Mice deficient for both SR-AI and SR-AII (scavenger receptors expressed by Kupffer and liver endothelial cells) and wild-type mice are also equally susceptible to malaria infection by P. berghei sporozoites61. Many pathogens can invade host cells using multiple, alternative pathways, with significant redundancy. This is true, for example, for red-blood-cell invasion by P. falciparum merozoites62. Therefore, it is plausible that, for hepatocyte invasion, other players exist that might be difficult to identify owing to redundancy. Whether any of the receptors mentioned above do have specific functions in malaria infection remains to be elucidated.
Developing in the hepatocyte
After the final invasion, each Plasmodium sporozoite develops and multiplies inside the hepatocyte, thereby generating thousands of new parasites (merozoites). However, relatively little is known about the cellular and molecular interactions in either the parasite or the host during this part of the life cycle. Recently, three distinct P. berghei proteins, the removal of which leads to an impairment of parasite development in hepatocytes, have been identified. These are UIS3 and UIS4 (UIS stands for upregulated in infective sporozoites) and Pb36p (Refs 63–65). As mentioned earlier, Pb36p-deficient parasites have also been reported to have a deficiency in terms of hepatocyte invasion54. Van Dijk et al.65 suggested that this protein is involved in parasite development because Pb36p-deficient sporozoites are mainly found inside cells that do not show signs of wounding and, therefore, have presumably entered the cell with the formation of a parasitophorous vacuole. The mutant parasite deficient for Pb36p obtained by Ishino et al.54 can also be found inside non-wounded cells, but in a much smaller proportion than that observed for wild-type parasites (approximately 10% for Pb36p-deficient parasites compared with 60% for wild-type parasites). Therefore, it seems that most of these mutant sporozoites cannot invade host cells. It is likely that Pb36p-deficient parasites are impaired in both invasion of the host cell and intracellular development. Interestingly, in the Pb36p mutant obtained by Ishino et al., the lack of infectivity leads to the continuous traversal of hepatocytes, which results in a 5–6-fold increase in the proportion of wounded cells, a phenotype not observed in the Pb36p-deficient parasites obtained by van Dijk et al.65 The reasons for these differences are presently unknown, and the role of these parasite molecules during Plasmodium development in hepatocytes is still unclear.
Immunization with any of these mutant parasites (deficient in UIS3, UIS4 or Pb36p) confers full protection against a subsequent challenge with fully infective P. berghei sporozoites63,64,65. Therefore, genetically attenuated sporozoites, as has previously been observed for radiation-attenuated sporozoites66, can induce protective immune responses and might be useful in a future vaccination strategy (Box 1).
As was mentioned above, HGF/MET signalling increases hepatocyte infection51. The mechanism behind this is not fully understood but it seems to involve protecting the host cell from apoptosis67 and, potentially, rearrangement of the host-cell cytoskeleton51. In fact, infection of host cells by P. berghei sporozoites confers a significant level of protection against apoptosis67,68, which decreases when hepatocytes are infected with irradiated sporozoites69. Indeed, the uptake of apoptotic infected hepatocytes by macrophages and dendritic cells in the liver was observed and has been discussed as a possible pathway for the presentation of sporozoite antigens69,70. At an early stage of development, HGF/MET signalling seems to have an important role in inducing this protective effect. However, additional ways of inhibiting host-cell apoptosis during parasite development probably exist and complement the effects of HGF.
Another host molecule that seems to have an important role in intrahepatocyte development is ApoA1, which localizes at the parasitophorous vacuole 24 hours post-infection, and seems to interact with the parasite UIS4 protein (A.-K. Mueller and K. Matuschewski, personal communication). One problem that any intracellular pathogen faces is how to create enough space for replication, with considerable expansion of the parasitophorous vacuole required to allow intra-vacuolar replication of malaria sporozoites. Such an increase in vacuole size requires the synthesis of large amounts of additional membrane and it is possible that ApoA1 might have a role in this process.
Leaving the hepatocyte
The final important step in the lifecycle of intracellular pathogens or pathogens with intracellular stages is the exit from the host cell after replication, but the molecular mechanisms involved in this process are still poorly understood. The release of Plasmodium merozoites from hepatocytes is usually referred to as occurring after hepatocyte rupture, but this has never been directly observed and the signal(s) that trigger the exit remain unknown. During erythrocyte infection, the rupture of the vacuole containing Plasmodium merozoites occurs before the rupture of the erythrocyte plasma membrane71, and distinct proteases are involved in each of these steps71,72. Recently, it has been reported that P. berghei merozoites are not released by the rupture of the hepatocyte but by the formation of merozoite-filled vesicles (merosomes), which bud off from the infected hepatocytes into the lumen of the liver sinusoids7 (Fig. 4). Initially, merozoites are released from the parasitophorous vacuole membrane and mix freely with the host-cell cytoplasm7,73. Interestingly, although the merozoite-containing host cell has apoptotic features, the amount of phosphatidylserine on its surface does not increase. The authors suggest that, in this way, the parasite manipulates the host cell to prevent infected hepatocytes from being phagocytosed. Moreover, the merosomes, the plasma membranes of which are of host-cell origin and therefore should not be recognized by dendritic cells or Kupffer cells, guide the merozoites safely into the bloodstream7. The molecular details of this process are not yet fully understood but it has been shown that proteases mediate the liberation of merozoites from the parasitophorous vacuole and the formation of the merosomes7. Merosome-like structures released into the sinusoids have also recently been reported for P. yoelii8. Whether similar features exist in P. falciparum remains unknown.
The biological events that occur between the bite of a malaria-infected mosquito and the release of Plasmodium merozoites into the host bloodstream are obligatory steps in the establishment of a malaria infection. Recently, our knowledge of the initial steps of the infection process after the injection of Plasmodium sporozoites into the skin has increased. Also, we now have a greater understanding of how sporozoites traverse the liver sinusoids and reach the hepatocytes (Timeline), and it is likely that Plasmodium sporozoites have developed multiple ways to achieve this goal. Detailed information is still lacking on the host and parasite molecules responsible for Plasmodium development within hepatocytes and the exit of merozoites from these cells.
As sporozoite multiplication in the liver is not associated with pathology, this stage of malaria infection is not a target for therapy. However, it is the most appealing stage for vaccine and prophylaxis strategies as, if infection is blocked at this stage, there will be no pathology and therefore no disease. In terms of prophylaxis, the main concerns regarding this strategy are drug resistance if the drug target is parasite-derived, and toxicity if the drug target is host-derived. With increasing knowledge of the molecular mechanisms underlying parasite development in the liver, it might become possible to design drugs that do not interfere with normal liver functions but do prevent infection.
Greenwood, B. & Mutabingwa, T. Malaria in 2002. Nature 415, 670–672 (2002).
Amino, R. et al. Quantitative imaging of Plasmodium transmission from mosquito to mammal. Nature Med. 12, 220–224 (2006). This paper describes intravital observations of Plasmodium sporozoites in the skin, and elucidates the importance of blood and lymph circulation during these early stages of infection.
Medica, D. L. & Sinnis, P. Quantitative dynamics of Plasmodium yoelii sporozoite transmission by infected anopheline mosquitoes. Infect. Immun. 73, 4363–4369 (2005).
Ponnudurai, T. et al. Sporozoite load of mosquitoes infected with Plasmodium falciparum. Trans. R. Soc. Trop. Med. Hyg. 83, 67–70 (1989).
Rosenberg, R., Wirtz, R. A., Schneider, I. & Burge, R. An estimation of the number of malaria sporozoites ejected by a feeding mosquito. Trans. R. Soc. Trop. Med. Hyg. 84, 209–212 (1990).
Despommier, D., Gwadz, R. W., Hotez, P. J. & Knirsch, C. A. Parasitic Diseases (Apple Trees Productions, New York, 2000).
Sturm, A. et al. Manipulation of host hepatocytes by the malaria parasite for delivery into liver sinusoids. Science 313, 1287–1290 (2006). This paper gives the first description of how P.berghei liver merozoites are not released in the bloodstream by hepatocyte rupture, but are instead contained in vesicles called merosomes that bud off from the infected hepatocyte into the lumen of liver sinusoids.
Tarun, A. S. et al. Quantitative isolation and in vivo imaging of malaria parasite liver stages. Int. J. Parasitol. 36, 1283–1293 (2006).
Stewart, M. J. & Vanderberg, J. P. Malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility. J. Protozool. 35, 389–393 (1988).
Mota, M. M. et al. Migration of Plasmodium sporozoites through cells before infection. Science 291, 141–144 (2001).
Sultan, A. A. et al. TRAP is necessary for gliding motility and infectivity of Plasmodium sporozoites. Cell 90, 511–522 (1997). References 9 and 11 describe the importance of two sporozoite surface molecules, CSP and TRAP, in sporozoite gliding motility. Reference 10 describes, for the first time, how sporozoites use gliding motility to traverse hepatocytes prior to infecting a final hepatocyte with the formation of a parasitophorous vacuole.
Meis, J. F., Verhave, J. P., Jap, P. H., Sinden, R. E. & Meuwissen, J. H. Ultrastructural observations on the infection of rat liver by Plasmodium berghei sporozoites in vivo. J. Protozool. 30, 361–366 (1983).
Cogswell, F. B. The hypnozoite and relapse in primate malaria. Clin. Microbiol. Rev. 5, 26–35 (1992).
Belmonte, M. et al. The infectivity of Plasmodium yoelii in different strains of mice. J. Parasitol. 89, 602–603 (2003).
Belnoue, E. et al. Protective T-cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment. J. Immunol. 172, 2487–2495 (2004).
Jaffe, R. I., Lowell, G. H. & Gordon, D. M. Differences in susceptibility among mouse strains to infection with Plasmodium berghei (ANKA clone) sporozoites and its relationship to protection by γ-irradiated sporozoites. Am. J. Trop. Med. Hyg. 42, 309–313 (1990).
van Dijk, M. R., Janse, C. J. & Waters, A. P. Expression of a Plasmodium gene introduced into subtelomeric regions of Plasmodium berghei chromosomes. Science 271, 662–665 (1996).
Mota, M. M., Thathy, V., Nussenzweig, R. S. & Nussenzweig, V. Gene targeting in the rodent malaria parasite Plasmodium yoelii. Mol. Biochem. Parasitol. 113, 271–278 (2001).
Griffiths, R. B. & Gordon, R. M. An apparatus which enables the process of feeding by mosquitoes to be observed in the tissues of a live rodent; together with an account of the ejection of saliva and its significance in malaria. Ann. Trop. Med. Parasitol. 46, 311–319 (1952).
Ponnudurai, T., Lensen, A. H., van Gemert, G. J., Bolmer, M. G. & Meuwissen, J. H. Feeding behaviour and sporozoite ejection by infected Anopheles stephensi. Trans. R. Soc. Trop. Med. Hyg. 85, 175–180 (1991).
Sidjanski, S. & Vanderberg, J. P. Delayed migration of Plasmodium sporozoites from the mosquito bite site to the blood. Am. J. Trop. Med. Hyg. 57, 426–429 (1997).
Vanderberg, J. P. & Frevert, U. Intravital microscopy demonstrating antibody-mediated immobilisation of Plasmodium berghei sporozoites injected into skin by mosquitoes. Int. J. Parasitol. 34, 991–996 (2004). References 20 to 22 report on the fact that sporozoites are placed in the skin of the host by the Anopheles mosquito rather than directly into the circulation.
Krettli, A. U. & Dantas, L. A. Which routes do Plasmodium sporozoites use for successful infections of vertebrates? Infect. Immun. 68, 3064–3065 (2000).
Vaughan, J. A., Scheller, L. F., Wirtz, R. A. & Azad, A. F. Infectivity of Plasmodium berghei sporozoites delivered by intravenous inoculation versus mosquito bite: implications for sporozoite vaccine trials. Infect. Immun. 67, 4285–4289 (1999).
Bhanot, P., Schauer, K., Coppens, I. & Nussenzweig, V. A surface phospholipase is involved in the migration of plasmodium sporozoites through cells. J. Biol. Chem. 280, 6752–6760 (2005).
Shin, S. C., Vanderberg, J. P. & Terzakis, J. A. Direct infection of hepatocytes by sporozoites of Plasmodium berghei. J. Protozool. 29, 448–454 (1982).
Wisse, E., De Zanger, R. B., Charels, K., Van Der Smissen, P. & McCuskey, R. S. The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse. Hepatology 5, 683–692 (1985).
Cerami, C. et al. The basolateral domain of the hepatocyte plasma membrane bears receptors for the circumsporozoite protein of Plasmodium falciparum sporozoites. Cell 70, 1021–1033 (1992).
Frevert, U. et al. Malaria circumsporozoite protein binds to heparan sulfate proteoglycans associated with the surface membrane of hepatocytes. J. Exp. Med. 177, 1287–1298 (1993).
Rathore, D., Sacci, J. B., de la Vega, P. & McCutchan, T. F. Binding and invasion of liver cells by Plasmodium falciparum sporozoites. Essential involvement of the amino terminus of circumsporozoite protein. J. Biol. Chem. 277, 7092–7098 (2002).
Sinnis, P. et al. Structural and functional properties of region II-plus of the malaria circumsporozoite protein. J. Exp. Med. 180, 297–306 (1994).
Sinnis, P., Willnow, T. E., Briones, M. R., Herz, J. & Nussenzweig, V. Remnant lipoproteins inhibit malaria sporozoite invasion of hepatocytes. J. Exp. Med. 184, 945–954 (1996).
Ying, P. et al. The malaria circumsporozoite protein: interaction of the conserved regions I and II-plus with heparin-like oligosaccharides in heparan sulfate. Exp. Parasitol. 85, 168–182 (1997).
Lyon, M., Deakin, J. A. & Gallagher, J. T. Liver heparan sulfate structure. A novel molecular design. J. Biol. Chem. 269, 11208–11215 (1994).
Pinzon-Ortiz, C., Friedman, J., Esko, J. & Sinnis, P. The binding of the circumsporozoite protein to cell surface heparan sulfate proteoglycans is required for plasmodium sporozoite attachment to target cells. J. Biol. Chem. 276, 26784–26791 (2001).
Pradel, G., Garapaty, S. & Frevert, U. Proteoglycans mediate malaria sporozoite targeting to the liver. Mol. Microbiol. 45, 637–651 (2002).
Pradel, G., Garapaty, S. & Frevert, U. Kupffer and stellate cell proteoglycans mediate malaria sporozoite targeting to the liver. Comp. Hepatol. 3, (Suppl. 1), S47 (2004).
Frevert, U., Usynin, I., Baer, K. & Klotz, C. Nomadic or sessile: can Kupffer cells function as portals for malaria sporozoites to the liver? Cell. Microbiol. 8, 1537–1546 (2006).
Pradel, G. & Frevert, U. Malaria sporozoites actively enter and pass through rat Kupffer cells prior to hepatocyte invasion. Hepatology 33, 1154–1165 (2001).
Frevert, U. et al. Intravital observation of Plasmodium berghei sporozoite infection of the liver. PLoS Biol. 3, e192 (2005). This paper describes the first intravital observations of Plasmodium sporozoites on reaching the liver. These observations confirmed the importance of several sporozoite features for infection, namely gliding motility and cell traversion, and of Kupffer cells as the most likely gates for parasite entry into the liver.
Ishino, T., Chinzei, Y. & Yuda, M. A Plasmodium sporozoite protein with a membrane attack complex domain is required for breaching the liver sinusoidal cell layer prior to hepatocyte infection. Cell. Microbiol. 7, 199–208 (2005).
Ishino, T., Yano, K., Chinzei, Y. & Yuda, M. Cell-passage activity is required for the malarial parasite to cross the liver sinusoidal cell layer. PLoS Biol. 2, e4 (2004). References 41 and 42 describe the features of sporozoites that are deficient in proteins that are essential for cell traversion. The results in these reports question not only the importance of cell traversion for efficient hepatocyte infection but also the concept of vacuole formation on sporozoite passage through Kupffer cells.
Silvie, O. et al. Hepatocyte CD81 is required for Plasmodium falciparum and Plasmodium yoelii sporozoite infectivity. Nature Med. 9, 93–96 (2003).
Kariu, T., Ishino, T., Yano, K., Chinzei, Y. & Yuda, M. CelTOS, a novel malarial protein that mediates transmission to mosquito and vertebrate hosts. Mol. Microbiol. 59, 1369–1379 (2006).
Mota, M. M., Hafalla, J. C. & Rodriguez, A. Migration through host cells activates Plasmodium sporozoites for infection. Nature Med. 8, 1318–1322 (2002). This paper shows that sporozoite traversion of hepatocytes activates the parasites for the invasion of a final hepatocyte with vacuole formation.
Gantt, S. et al. Antibodies against thrombospondin-related anonymous protein do not inhibit Plasmodium sporozoite infectivity in vivo. Infect. Immun. 68, 3667–3673 (2000).
Carruthers, V. B. & Sibley, L. D. Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts. Eur. J. Cell Biol. 73, 114–123 (1997).
Coppi, A., Pinzon-Ortiz, C., Hutter, C. & Sinnis, P. The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion. J. Exp. Med. 201, 27–33 (2005).
Mota, M. M. & Rodriguez, A. Migration through host cells: the first steps of Plasmodium sporozoites in the mammalian host. Cell. Microbiol. 6, 1113–1118 (2004).
Yuda, M. & Ishino, T. Liver invasion by malarial parasites — how do malarial parasites break through the host barrier? Cell. Microbiol. 6, 1119–1125 (2004).
Carrolo, M. et al. Hepatocyte growth factor and its receptor are required for malaria infection. Nature Med. 9, 1363–1369 (2003).
Matuschewski, K., Nunes, A. C., Nussenzweig, V. & Menard, R. Plasmodium sporozoite invasion into insect and mammalian cells is directed by the same dual binding system. EMBO J. 21, 1597–1606 (2002).
Silvie, O. et al. A role for apical membrane antigen 1 during invasion of hepatocytes by Plasmodium falciparum sporozoites. J. Biol. Chem. 279, 9490–9496 (2004).
Ishino, T., Chinzei, Y. & Yuda, M. Two proteins with 6-cys motifs are required for malarial parasites to commit to infection of the hepatocyte. Mol. Microbiol. 58, 1264–1275 (2005).
Jethwaney, D. et al. Fetuin-A, a hepatocyte-specific protein that binds Plasmodium berghei thrombospondin-related adhesive protein: a potential role in infectivity. Infect. Immun. 73, 5883–5891 (2005).
Pileri, P. et al. Binding of hepatitis C virus to CD81. Science 282, 938–941 (1998).
Silvie, O. et al. Cholesterol contributes to the organization of tetraspanin-enriched microdomains and to CD81-dependent infection by malaria sporozoites. J. Cell Sci. 119, 1992–2002 (2006).
Sultan, A. A., Briones, M. R., Gerwin, N., Carroll, M. C. & Nussenzweig, V. Sporozoites of Plasmodium yoelii infect mice with targeted deletions in ICAM-1 and ICAM-2 or complement components C3 and C4. Mol. Biochem. Parasitol. 88, 263–266 (1997).
Bhanot, P. & Nussenzweig, V. Plasmodium yoelii sporozoites infect Syndecan-1 deficient mice. Mol. Biochem. Parasitol. 123, 143–144 (2002).
Sinnis, P. & Febbraio, M. Plasmodium yoelii sporozoites infect CD36-deficient mice. Exp. Parasitol. 100, 12–16 (2002).
Cunha-Rodrigues, M., Portugal, S., Febbraio, M. & Mota, M. M. Infection by and protective immune responses against Plasmodium berghei ANKA are not affected in macrophage scavenger receptors A deficient mice. BMC Microbiol. 6, 73 (2006).
Oh, S. S. & Chishti, A. H. Host receptors in malaria merozoite invasion. Curr. Top. Microbiol. Immunol. 295, 203–232 (2005).
Mueller, A. K. et al. Plasmodium liver stage developmental arrest by depletion of a protein at the parasite-host interface. Proc. Natl Acad. Sci. USA 102, 3022–3027 (2005).
Mueller, A. K., Labaied, M., Kappe, S. H. & Matuschewski, K. Genetically modified Plasmodium parasites as a protective experimental malaria vaccine. Nature 433, 164–167 (2005).
van Dijk, M. R. et al. Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells. Proc. Natl Acad. Sci. USA 102, 12194–12199 (2005). References 63 to 65 describe genetic modifications of Plasmodium parasites that prevent their intra-hepatocytic development and confer protective immunity against subsequent infections.
Nussenzweig, R. S., Vanderberg, J., Most, H. & Orton, C. Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. Nature 216, 160–162 (1967).
Leiriao, P. et al. HGF/MET signalling protects Plasmodium-infected host cells from apoptosis. Cell. Microbiol. 7, 603–609 (2005).
van de Sand, C. et al. The liver stage of Plasmodium berghei inhibits host cell apoptosis. Mol. Microbiol. 58, 731–742 (2005).
Leiriao, P., Mota, M. M. & Rodriguez, A. Apoptotic Plasmodium-infected hepatocytes provide antigens to liver dendritic cells. J. Infect. Dis. 191, 1576–1581 (2005).
Renia, L. et al. Do apoptotic Plasmodium-infected hepatocytes initiate protective immune responses? J. Infect. Dis. 193, 163–164; author reply 164–165 (2006).
Wickham, M. E., Culvenor, J. G. & Cowman, A. F. Selective inhibition of a two-step egress of malaria parasites from the host erythrocyte. J. Biol. Chem. 278, 37658–37663 (2003).
Salmon, B. L., Oksman, A. & Goldberg, D. E. Malaria parasite exit from the host erythrocyte: a two-step process requiring extraerythrocytic proteolysis. Proc. Natl Acad. Sci. USA 98, 271–276 (2001).
Meis, J. F., Verhave, J. P., Jap, P. H. & Meuwissen, J. H. Fine structure of exoerythrocytic merozoite formation of Plasmodium berghei in rat liver. J. Protozool. 32, 694–699 (1985).
Clyde, D. F., McCarthy, V. C., Miller, R. M. & Hornick, R. B. Specificity of protection of man immunized against sporozoite-induced falciparum malaria. Am. J. Med. Sci. 266, 398–403 (1973).
Herrington, D. A. et al. Safety and immunogenicity of a recombinant sporozoite malaria vaccine against Plasmodium vivax. Am. J. Trop. Med. Hyg. 45, 695–701 (1991).
Hoffman, S. L. et al. Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites. J. Infect. Dis. 185, 1155–1164 (2002).
Rieckmann, K. H., Carson, P. E., Beaudoin, R. L., Cassells, J. S. & Sell, K. W. Letter: Sporozoite induced immunity in man against an Ethiopian strain of Plasmodium falciparum. Trans. R. Soc. Trop. Med. Hyg. 68, 258–259 (1974).
Luke, T. C. & Hoffman, S. L. Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated Plasmodium falciparum sporozoite vaccine. J. Exp. Biol. 206, 3803–3808 (2003).
Menard, R. Knockout malaria vaccine? Nature 433, 113–114 (2005).
Waters, A. P., Mota, M. M., van Dijk, M. R. & Janse, C. J. Parasitology. Malaria vaccines: back to the future? Science 307, 528–530 (2005).
Alonso, P. L. et al. Efficacy of the RTS, S/AS02A vaccine against Plasmodium falciparum infection and disease in young African children: randomised controlled trial. Lancet 364, 1411–1420 (2004).
Cunha-Rodrigues, M., Prudêncio, M., Mota, M. M. & Haas, W. Antimalarial drugs — host targets (re)visited. Biotechnol. J. 1, 321–332 (2006).
Ross, R. On some peculiar pigmented cells found in two mosquitoes fed on malaria blood. Br. Med. J. 2, 1786–1788 (1897).
Grassi, B., Bignami, A. & Bastienelli, G. Ulteriori richerchi sul ciclo dei parassiti malarici umani nel corpo del zanzarone. Atti R. Accad. Lincei. 5, 21–28 (1899).
Shortt, H. E. & Garnham, P. C. C. Demonstration of a persisting exo-erythrocytic cycle in Plasmodium cynomolgi and its bearing on the production of relapse. Br. Med. J. 1, 1222–1228 (1948).
Garnham, P. C. C. The continuing mystery of relapses in malaria. Protozool. Abstr. 1, 1–12 (1977).
Hollingdale, M. R., Leef, J. L., McCullough, M. & Beaudoin, R. L. In vitro cultivation of the exoerythrocytic stage of Plasmodium berghei from sporozoites. Science 213, 1021–1022 (1981).
Ozaki, L. S. et al. Structure of the Plasmodium knowlesi gene coding for the circumsporozoite protein. Cell 34, 815–822 (1983).
Kappe, S. H. et al. Exploring the transcriptome of the malaria sporozoite stage. Proc. Natl Acad. Sci. USA 98, 9895–9900 (2001).
The authors declare no competing financial interests.
Entrez Genome Project
The system of blood-containing spaces pervading the body in the mosquito.
- Gliding motility
A form of substrate-dependent locomotion in which the parasite maintains a fixed shape.
- Parasitophorous vacuole
A vacuole within the host cell in which the parasite resides.
- Intravital microscopy
The direct real-time imaging of biological phenomena in exposed tissues.
- Dendritic cell
A 'professional' antigen-presenting cell that is found in the T-cell areas of lymphoid tissues and as a minor cellular component in most tissues. Dendritic cells have a branched or dendritic morphology and are the most potent stimulators of T-cell responses.
A small, fenestrated blood vessel found in the periphery of the lobules of the liver. Sinusoids are lined with endothelial and Kupffer cells.
- Heparan sulphate proteoglycan
(HSPG). A family of glycoproteins that function as co-receptors for growth factors and matrix proteins. They are present on the cell surfaces and in the extracellular matrix of most mammalian cells. In the liver, HSPGs are thought to either directly internalize bound lipoproteins by hepatocytes or to localize lipoproteins to the cell surface.
- Kupffer cell
A macrophage, or monocytic cell, that is permanently located in the liver between sinusoidal endothelial cells. Kupffer cells form the largest population of macrophages in the body.
- Stellate cell
A highly branched peri-sinusoidal cell, which is the principal producer of the extracellular matrix in the liver that contributes to hepatic inflammation through the secretion of chemokines and the recruitment of leukocytes.
- 6-Cys domain protein family
A family of ten proteins characterized by a pattern of conserved cysteine residues that are unique to Plasmodium species.
The tetraspanin protein family contains proteins that span the membrane four times with two exoplasmic loops, and that can be found at the cell surface.
Rights and permissions
About this article
Cite this article
Prudêncio, M., Rodriguez, A. & Mota, M. The silent path to thousands of merozoites: the Plasmodium liver stage. Nat Rev Microbiol 4, 849–856 (2006). https://doi.org/10.1038/nrmicro1529
This article is cited by
Translation of liver stage activity of M5717, a Plasmodium elongation factor 2 inhibitor: from bench to bedside
Malaria Journal (2022)
A genetically modified Plasmodium berghei parasite as a surrogate for whole-sporozoite vaccination against P. vivax malaria
npj Vaccines (2022)
Plasmodium—a brief introduction to the parasites causing human malaria and their basic biology
Journal of Physiological Anthropology (2021)
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins
Malaria Journal (2021)
Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP
Scientific Reports (2021)