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Reverse-genetic approaches to the study of Borna disease virus

Nature Reviews Microbiology volume 4, pages 777–783 (2006)Cite this article

Abstract

Borna disease virus (BDV) is an enveloped virus that has a non-segmented, negative-strand RNA genome with the characteristic organization of the mononegaviruses. However, based on its unique genetic and biological features, BDV is considered to be the prototypic member of a new mononegavirus family, the Bornaviridae. BDV causes central nervous system (CNS) disease in a wide variety of mammals. This article discusses the recently developed reverse-genetics systems for BDV, and the implications for the elucidation of the molecular mechanisms underlying BDV–host interactions, including the basis of BDV persistence in the CNS and its associated diseases.

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Figure 1: Borna disease virus genome organization.
Figure 2: The Borna disease virus lifecycle.
Figure 3: Borna disease virus minigenome rescue systems.
Figure 4: General scheme for the rescue of recombinant Borna disease virus from cloned cDNA.

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Acknowledgements

Work conducted in J.C.T.'s laboratory was supported by a National Institutes of Health grant.

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  1. Molecular Integrative Neuroscience Department IMM-6, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, 92037, California, USA

    Juan C. de la Torre

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de la Torre, J. Reverse-genetic approaches to the study of Borna disease virus. Nat Rev Microbiol 4, 777–783 (2006). https://doi.org/10.1038/nrmicro1489

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