Review

Functional horizontal gene transfer from bacteria to eukaryotes

Published online:

Abstract

Bacteria influence eukaryotic biology as parasitic, commensal or beneficial symbionts. Aside from these organismal interactions, bacteria have also been important sources of new genetic sequences through horizontal gene transfer (HGT) for eukaryotes. In this Review, we focus on gene transfers from bacteria to eukaryotes, discuss how horizontally transferred genes become functional and explore what functions are endowed upon a broad diversity of eukaryotes by genes derived from bacteria. We classify HGT events into two broad types: those that maintain pre-existing functions and those that provide the recipient with new functionality, including altered host nutrition, protection and adaptation to extreme environments.

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Acknowledgements

F.H. was supported by the Fulbright Commission and a fellowship from the European Molecular Biology Organization (EMBO; ALTF 1260–2016) while writing this Review. J.P.M. was supported by grants from the US National Science Foundation (IOS-1256680 and IOS-1553529), the US National Aeronautics and Space Administration (NASA) Astrobiology Institute (NNA15BB04A) and the Gordon and Betty Moore Foundation (GBMF5602).

Author information

Author notes

    • Filip Husnik

    Present address: Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.

Affiliations

  1. Faculty of Science, University of South Bohemia and Institute of Parasitology, Biology Centre Academy of Sciences of the Czech Republic (ASCR), Ceske Budejovice 370 05, Czech Republic.

    • Filip Husnik
  2. Division of Biological Sciences, University of Montana, Missoula, Montana 59812, USA.

    • Filip Husnik
    •  & John P. McCutcheon

Authors

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Contributions

F.H. and J.P.M. researched data for the article, made substantial contributions to discussions of the content, wrote the article and reviewed and/or edited the manuscript before submission.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Filip Husnik or John P. McCutcheon.

Supplementary information

Excel files

  1. 1.

    Supplementary information S1 (table)

    HGT events from bacteria to eukaryotes.

Glossary

Horizontal gene transfer

(HGT). Movement of genetic material between organisms (also called lateral gene transfer) via non-vertical (not parent-to-offspring) transmission.

Germ line

The specialized cellular lineage in multicellular sexual organisms that is used to pass on genetic material to the progeny.

Endosymbionts

Organisms living within the body or cells of another organism.

Long-branch attraction

A phylogenetic artefact that causes distantly related lineages (often on long branches) to be incorrectly inferred as closely related in phylogenetic trees.

Conjugation

Gene transfer from a donor to a recipient by direct cell-to-cell contact, such as plasmid transfer between two bacterial cells.

Transduction

Gene transfer carried out by a virus, such as a bacteriophage, transferring DNA from one bacterium to another.

Transformation

Direct acquisition of DNA from the environment through the cell membrane.

Gene transfer agents

Bacteriophage-like elements that package random DNA regions from a host cell and transfer them to a recipient cell.

Non-homologous end joining

(NHEJ). A pathway for the direct repair of double-strand DNA breaks without a homologous template.

Nuclear mitochondrial transfers

(numts). Transfers of mitochondrial DNA into the nuclear genome of a eukaryotic host; the encoded genes often become non-functional.

Nuclear plastid transfers

(nupts). Transfers of plastid DNA into the nuclear genome of a eukaryotic host; the encoded genes often become non-functional.

Transposable elements

DNA sequences (also known as jumping genes) that can move within a genome (and sometimes also between genomes) by a 'cut and paste' (DNA transposons) or a 'copy and paste' (retrotransposons) mechanism.

Organellogenesis

The process by which an endosymbiont becomes an organelle, in part by becoming (nearly) irreversibly integrated with its host cell at both a genetic and cell biological level.

Reproductive manipulators

Bacteria, such as Wolbachia spp., that are transmitted in the egg cytoplasm of arthropods and nematodes and shift the sex ratio of the host population.

Peptidoglycan

A structural matrix in bacterial cell walls formed by alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) residues, where peptide chains of up to five amino acids link NAM to other NAM-connected peptides.