Abstract
Differentiated cells can be experimentally reprogrammed back to pluripotency by nuclear transfer, cell fusion or induced pluripotent stem cell technology. Nuclear transfer and cell fusion can lead to efficient reprogramming of gene expression. The egg and oocyte reprogramming process includes the exchange of somatic proteins for oocyte proteins, the post-translational modification of histones and the demethylation of DNA. These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes.
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Acknowledgements
V.P. is supported by a Wallonia-Brussels International Excellence Grant. R.P.H.-S. is supported by the National Research Foundation of South Africa and the Cambridge Commonwealth Trust, UK. K.M. is supported by the Japan Society for the Promotion of Science (International Research Fellowship Program). This work was also supported by the Wellcome Trust (RHG54943).
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Jullien, J., Pasque, V., Halley-Stott, R. et al. Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process?. Nat Rev Mol Cell Biol 12, 453–459 (2011). https://doi.org/10.1038/nrm3140
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DOI: https://doi.org/10.1038/nrm3140
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