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Photoactivatable fluorescent proteins

Nature Reviews Molecular Cell Biologyvolume 6pages885890 (2005) | Download Citation



The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.

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The authors are supported by the European Commission Framework Program 6 and the Russian Academy of Sciences for the programme in Molecular and Cell Biology (K.A.L., D.M.C. and S.L.), and the National Institute of General Medical Sciences and the National Institute on Drug Abuse (V.V.V.).

Author information


  1. the Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    • Konstantin A. Lukyanov
    • , Dmitry M. Chudakov
    •  & Sergey Lukyanov
  2. the Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, 80045, Colorado, USA

    • Vladislav V. Verkhusha


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The authors declare no competing financial interests.

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Correspondence to Konstantin A. Lukyanov or Vladislav V. Verkhusha.

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