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The maturing role of COPI vesicles in intra-Golgi transport

Nature Reviews Molecular Cell Biology volume 6, pages 812–817 (2005)Cite this article

Abstract

COPI vesicles that surround the Golgi stack were first implicated in the anterograde movement of cargo, and then in the retrograde movement of Golgi enzymes. Recently, their role has been challenged again, and we discuss new data that both confirm and modify our view of these carriers.

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Figure 1: An electron microscopy image of a Golgi region in a high-pressure-frozen and freeze-substituted D1 dendritic cell.
Figure 2: A schematic representation of four different models of intra-Golgi transport.
Figure 3: An overview of the proposed roles of COPI vesicles.
Figure 4: A three-dimensional tomographic reconstruction of a Golgi stack in a high-pressure-frozen and freeze-substituted HepG2 hepatoma cell.

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Acknowledgements

Our thanks to J. L. Murk and H. Geuze (Department of Cell Biology, University Medical Center Utrecht, The Netherlands) and A. J. Koster (Department of Molecular Cell Biology, Faculty of Biology, Utrecht University, The Netherlands) for the electron microscopy figures and the Supplementary information S1 (movie). We especially thank W. J. Geerts (Department of Molecular Cell Biology, Faculty of Biology, Utrecht University, The Netherlands) for the acquisition of tomograms, and also T. Nilsson and G. Warren for sharing unpublished data. We apologize to our colleagues who have contributed to our understanding of the Golgi but whose work could not be mentioned in this article due to space constraints.

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  1. Department of Cell Biology and Institute of Biomembranes, The Cell Microscopy Center, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands

    Catherine Rabouille & Judith Klumperman

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  1. Catherine Rabouille
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Correspondence to Judith Klumperman.

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Supplementary information

Supplementary information S1

Movie S1 | A movie of a Golgi stack. A movie of the Golgi stack that is shown in FIG. 4. The movie zooms in on the cis side of the Golgi (light blue in FIG. 4), and an arrow highlights two coatomer protein complex-I (COPI)-coated vesicles (shown in pink in FIG. 4). By going up and down through the reconstructed volume, it is clear that the highlighted structures are free COPI vesicles. This movie was kindly provided by Jean Luc Murk and Hans Geuze (Department of Cell Biology, University Medical Center Utrecht, The Netherlands) and Willie Geerts and Bram Koster (Department of Molecular Cell Biology, Faculty of Biology, Utrecht University, The Netherlands). (AVI 3634 kb)

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ARF1

Mannosidase II

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Rabouille, C., Klumperman, J. The maturing role of COPI vesicles in intra-Golgi transport. Nat Rev Mol Cell Biol 6, 812–817 (2005). https://doi.org/10.1038/nrm1735

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