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Oligosaccharide microarrays to decipher the glyco code

Nature Reviews Molecular Cell Biology volume 5, pages 582–588 (2004)Cite this article

Abstract

The oligosaccharide moieties of glycoproteins, glycolipids, proteoglycans and polysaccharides are highly diverse, the reason for this diversity is not yet understood. Neoglycolipid technology allows the generation of oligosaccharide probes with lipid tags from desired sources and is showing promise as a basis for oligosaccharide microarrays. Such microarrays would allow surveys of glycomes and proteomes to be carried out, which would enable the molecular definition of carbohydrate-recognition systems in whole organisms.

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Figure 1: Schematic representation of NGL technology.
Figure 2: NGL-based oligosaccharide microarrays.
Figure 3: Probing glycomes and proteomes for carbohydrate-recognition systems.

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Acknowledgements

The authors are supported by the Medical Research Council. A. M. Lawson, R. A. Childs, M. S. Stoll and C. Galustian are acknowledged for critically reading this manuscript and we would also like to thank P. Crocker for stimulating discussions. This article is dedicated to the late Elvin A Kabat.

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  1. The Glycosciences Laboratory, Imperial College London, Northwick Park Hospital Campus, Watford Road, Harrow, HA1 3UJ, Middlesex, UK

    Ten Feizi & Wengang Chai

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Correspondence to Ten Feizi.

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DATABASES

Swiss-Prot

E-selectin

Interferon-γ

L-selectin

P-selectin

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Feizi, T., Chai, W. Oligosaccharide microarrays to decipher the glyco code. Nat Rev Mol Cell Biol 5, 582–588 (2004). https://doi.org/10.1038/nrm1428

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