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Looking forward to seeing calcium

Nature Reviews Molecular Cell Biology volume 4, pages 579–586 (2003)Cite this article

Abstract

From molecules, single cells and tissues to whole organisms, our insights into Ca2+ signalling and the corresponding physiological phenomena are growing exponentially. Here, we describe the improvements that have been made in the development of the probes and instrumentation that are used for Ca2+ imaging and the expanding applications of Ca2+ imaging in basic and applied research.

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Figure 1: Procedure of a typical Ca2+ measurement experiment.
Figure 2: Fluorescence resonance energy transfer.
Figure 3: Examples of Ca2+-concentration signalling in living cells.
Figure 4: Description of major decisions for the planning of a Ca2+-concentration imaging experiment.

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Acknowledgements

The original work by the authors was supported by grants from the Italian Ministry of University, the Italian Research Council (CNR), Italian Telethon, the Italian Association for Cancer Research (AIRC), ASI, Human Science Frontier and from the European Union (EU) to T.P. and R. Rizzuto. R. Rudolf is supported by an EU Marie Curie fellowship. We are grateful to G. Carmignoto and L. Filippin for supplying artwork and to P. Magalhães for help with image processing.

Author information

Authors and Affiliations

  1. the Department of Biomedical Sciences, CNR Institute of Neurosciences, University of Padova, via G. Columbo 3, Padova, 35121, Italy

    Rüdiger Rudolf & Tullio Pozzan

  2. the Venetian Institute for Molecular Medicine, via Orus 2, Padova, 35129, Italy

    Marco Mongillo & Tullio Pozzan

  3. the Department of Experimental and Diagnostic Medicine, Section of General Pathology, University of Ferrara, via Borsari 46, Ferrara, 44100, Italy

    Rosario Rizzuto

Authors
  1. Rüdiger Rudolf
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  2. Marco Mongillo
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  3. Rosario Rizzuto
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  4. Tullio Pozzan
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Correspondence to Tullio Pozzan.

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FURTHER INFORMATION

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Venetian Institute of Molecular Medicine

Introduction to Ca2+ measurement with fluorescent indicators

Atsushi Miyawaki's laboratory

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Rudolf, R., Mongillo, M., Rizzuto, R. et al. Looking forward to seeing calcium. Nat Rev Mol Cell Biol 4, 579–586 (2003). https://doi.org/10.1038/nrm1153

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