Kotloff, K. L. et al. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull. World Health Organ 77, 651–666 (1999).
Zhang, W. et al. Wide dissemination of multidrug-resistant Shigella isolates in China. J. Antimicrob. Chemother. 66, 2527–2535 (2011).
Ghosh, S. et al. Genetic characteristics and changing antimicrobial resistance among Shigella spp. isolated from hospitalized diarrhoeal patients in Kolkata, India. J. Med. Microbiol. 60, 1460–1466 (2011).
Vinh, H. et al. A changing picture of shigellosis in southern Vietnam: shifting species dominance, antimicrobial susceptibility and clinical presentation. BMC Infect. Dis. 9, 204 (2009).
Khatun, F. et al. Changing species distribution and antimicrobial susceptibility pattern of Shigella over a 29-year period (1980–2008). Epidemiol. Infect. 139, 446–452 (2011).
Taneja, N., Mewara, A., Kumar, A., Verma, G. & Sharma, M. Cephalosporin-resistant Shigella flexneri over 9 years (2001–2009) in India. J. Antimicrob. Chemother. 67, 1347–1353 (2012).
Foster, R. A. et al. Structural elucidation of the O-antigen of the Shigella flexneri provisional serotype 88–893: structural and serological similarities with S. flexneri provisional serotype Y394 (1c). Carbohydr. Res. 346, 872–876 (2011).
Levine, M. M. et al. Pathogenesis of Shigella dysenteriae 1 (Shiga) dysentery. J. Infect. Dis. 127, 261–270 (1973).
Hyams, K. C. et al. Diarrheal disease during Operation Desert Shield. N. Engl. J. Med. 325, 1423–1428 (1991).
Kotloff, K. et al. The Global Enterics Multicenter Study (GEMS) of diarrheal disease in young children in developing countries: epidemiologic and clinical methods of the case/control study. Clin. Infect. Dis. 55 (Suppl. 4), S232–S245 (2012).
Panchalingam, S. et al. Diagnostic microbiologic methods in the GEMS-1 Case/Control Study. Clin. Infect. Dis. 55 (Suppl. 4), S294–S302 (2012).
Levine, M. M., Kotloff, K. L., Barry, E. M., Pasetti, M. F. & Sztein, M. B. Clinical trials of Shigella vaccines: two steps forward and one step back on a long, hard road. Nat. Rev. Microbiol. 5, 540–553 (2007).
Centers for Disease Control and Prevention. Health status of displaced persons following Cival War—Burundi, Dcemeber 1993–January 1994. MMWR Morb. Mortal. Wkly Rep. 43, 701–703 (1994).
Noriega, F. R. et al. Strategy for cross-protection among Shigella flexneri serotypes. Infect. Immun. 67, 782–788 (1999).
Jin, Q. et al. Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157. Nucleic Acids Res. 30, 4432–4441 (2002).
Yang, F. et al. Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery. Nucleic Acids Res. 33, 6445–6458 (2005).
Yang, J., Chen, L., Yu, J., Sun, L. & Jin, Q. ShiBASE: an integrated database for comparative genomics of Shigella. Nucleic Acids Res. 34, D398–D401 (2006).
Wei, J. et al. Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T. Infect. Immun. 71, 2775–2786 (2003).
Ying, T. Y. et al. Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T. World J. Gastroenterol. 11, 6880–6883 (2005).
Phalipon, A. & Sansonetti, P. J. Shigella's ways of manipulating the host intestinal innate and adaptive immune system: a tool box for survival? Immunol. Cell Biol. 85, 119–129 (2007).
Schroeder, G. N. & Hilbi, H. Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion. Clin. Microbiol. Rev. 21, 134–156 (2008).
DuPont, H. L., Hornick, R. B., Dawkins, A. T., Snyder, M. J. & Formal, S. B. The response of man to virulent Shigella flexneri 2a. J. Infect. Dis. 119, 296–299 (1969).
Taylor, D. N. et al. The role of Shigella spp., enteroinvasive Escherichia coli, and other enteropathogens as causes of childhood dysentery in Thailand. J. Infect. Dis. 153, 1132–1138 (1986).
Rout, W. R., Formal, S. B., Giannella, R. A. & Dammin, G. J. Pathophysiology of Shigella diarrhea in the rhesus monkey: intestinal transport, morphological, and bacteriological studies. Gastroenterology 68, 270–278 (1975).
Ashkenazi, S., Dinari, G., Zevulunov, A. & Nitzan, M. Convulsions in childhood shigellosis. Clinical and laboratory features in 153 children. Am. J. Dis. Child 141, 208–210 (1987).
Koster, F. et al. Hemolytic-uremic syndrome after shigellosis. Relation to endotoxemia and circulating immune complexes. N. Engl. J. Med. 298, 927–933 (1978).
Struelens, M. J. et al. Shigella septicemia: prevalence, presentation, risk factors, and outcome. J. Infect. Dis. 152, 784–790 (1985).
Nelson, M. R., Shanson, D. C., Hawkins, D. A. & Gazzard, B. G. Salmonella, Campylobacter and Shigella in HIV-seropositive patients. AIDS 6, 1495–1498 (1992).
Fasano, A., Noriega, F. R., Liao, F. M., Wang, W. & Levine, M. M. Effect of Shigella enterotoxin 1 (ShET1) on rabbit intestine in vitro and in vivo. Gut 40, 505–511 (1997).
Fasano, A. et al. Shigella enterotoxin 1: an enterotoxin of Shigella flexneri 2a active in rabbit small intestine in vivo and in vitro. J. Clin. Invest. 95, 2853–2861 (1995).
Farfan, M. J., Toro, C. S., Barry, E. M. & Nataro, J. P. Shigella enterotoxin-2 is a type III effector that participates in Shigella-induced interleukin 8 secretion by epithelial cells. FEMS Immunol. Med. Microbiol. 61, 332–339 (2011).
Kotloff, K. L. et al. Deletion in the Shigella enterotoxin genes further attenuates Shigella flexneri 2a bearing guanine auxotrophy in a phase 1 trial of CVD 1204 and CVD 1208. J. Infect. Dis. 190, 1745–1754 (2004).
Kotloff, K. L. et al. Safety and Immunogenicity of CVD 1208S, a live, oral ΔguaBA Δsen Δset Shigella flexneri 2a vaccine grown on animal-free media. Hum. Vaccin. 3, 268–275 (2007).
Ranallo, R. T. et al. Two live attenuated Shigella flexneri 2a strains WRSf2G12 and WRSf2G15: A new combination of gene deletions for 2nd generation live attenuated vaccine candidates. Vaccine 30, 5159–5171 (2012).
Bedford, L. et al. Further characterization of Shigella sonnei live vaccine candidates WRSs2 and WRSs3-plasmid composition, invasion assays and Sereny reactions. Gut Microbes 2, 244–251 (2011).
Dutta, P. R., Cappello, R., Navarro-Garcia, F. & Nataro, J. P. Functional comparison of serine protease autotransporters of Enterobacteriaceae. Infect. Immun. 70, 7105–7113 (2002).
Al-Hasani, K. et al. The sigA gene which is borne on the she pathogenicity island of Shigella flexneri 2a encodes an exported cytopathic protease involved in intestinal fluid accumulation. Infect. Immun. 68, 2457–2463 (2000).
Czerkinsky, C. & Kim, D. W. Novel Shigella protein antigens and methods. US Patent 8168203 [US2010/0136045] (2010).
Benjelloun-Touimi, Z., Si, T. M., Montecucco, C., Sansonetti, P. J. & Parsot, C. SepA, the 110 kDa protein secreted by Shigella flexneri: two-domain structure and proteolytic activity. Microbiology 144, 1815–1822 (1998).
Faherty, C. et al. Chromosomal and plasmid-encoded factors of Shigella flexneri induce secretogenic activity ex vivo. PLoS ONE 7, e49980 (2012).
Henderson, I. R., Czeczulin, J., Eslava, C., Noriega, F. & Nataro, J. P. Characterization of Pic, a secreted protease of Shigella flexneri and enteroaggregative Escherichia coli. Infect. Immun. 67, 5587–5596 (1999).
Behrens, M., Sheikh, J. & Nataro, J. P. Regulation of the overlapping pic/set locus in Shigella flexneri and enteroaggregative Escherichia coli. Infect. Immun. 70, 2915–2925 (2002).
Ruiz-Perez, F. et al. Serine protease autotransporters from Shigella flexneri and pathogenic Escherichia coli target a broad range of leukocyte glycoproteins. Proc. Natl Acad. Sci. USA 108, 12881–12886 (2011).
Boisen, N., Ruiz-Perez, F., Scheutz, F., Krogfelt, K. A. & Nataro, J. P. Short report: high prevalence of serine protease autotransporter cytotoxins among strains of enteroaggregative Escherichia coli. Am. J. Trop. Med. Hyg. 80, 294–301 (2009).
Formal, S. B. et al. Protection of monkeys against experimental shigellosis with a living attenuated oral polyvalent dysentery vaccine. J. Bacteriol. 92, 17–22 (1966).
Formal, S. B., LaBrec, E. H., Palmer, A. & Falkow, S. Protection of monkeys against experimental shigellosis with attenuated vaccines. J. Bacteriol. 90, 63–68 (1965).
Shipley, S. T. et al. A challenge model for Shigella dysenteriae 1 in cynomolgus monkeys (Macaca fascicularis). Comp. Med. 60, 54–61 (2010).
Etheridge, M. E., Hoque, A. T. & Sack, D. A. Pathologic study of a rabbit model for shigellosis. Lab. Anim. Sci. 46, 61–66 (1996).
Rabbani, G. H. et al. Development of an improved animal model of shigellosis in the adult rabbit by colonic infection with Shigella flexneri 2a. Infect. Immun. 63, 4350–4357 (1995).
Sereny, B. Experimental Shigella keratoconjunctivitis; a preliminary report. Acta Microbiol. Acad. Sci. Hung. 2, 293–296 (1955).
Mallett, C. P., VanDeVerg, L., Collins, H. H. & Hale, T. L. Evaluation of Shigella vaccine safety and efficacy in an intranasally challenged mouse model. Vaccine 11, 190–196 (1993).
Shim, D. H. et al. New animal model of shigellosis in the Guinea pig: its usefulness for protective efficacy studies. J. Immunol. 178, 2476–2482 (2007).
Nataro, J. P. et al. Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigella strains. Infect. Immun. 63, 4721–4728 (1995).
Formal, S. B. et al. Effect of prior infection with virulent Shigella flexneri 2a on the resistance of monkeys to subsequent infection with Shigella sonnei. J. Infect. Dis. 164, 533–537 (1991).
Herrington, D. A. et al. Studies in volunteers to evaluate candidate Shigella vaccines: further experience with a bivalent Salmonella typhi–Shigella sonnei vaccine and protection conferred by previous Shigella sonnei disease. Vaccine 8, 353–357 (1990).
Kotloff, K. L. et al. A modified Shigella volunteer challenge model in which the inoculum is administered with bicarbonate buffer: clinical experience and implications for Shigella infectivity. Vaccine 13, 1488–1494 (1995).
Ferreccio, C. et al. Epidemiologic patterns of acute diarrhea and endemic Shigella infections in children in a poor periurban setting in Santiago, Chile. Am. J. Epidemiol. 134, 614–627 (1991).
Passwell, J. H. et al. Safety and immunogenicity of Shigella sonnei-CRM9 and Shigella flexneri type 2a-rEPAsucc conjugate vaccines in one- to four-year-old children. Pediatr. Infect. Dis. J. 22, 701–706 (2003).
Robbins, J. B., Chu, C. & Schneerson, R. Hypothesis for vaccine development: protective immunity to enteric diseases caused by nontyphoidal salmonellae and shigellae may be conferred by serum IgG antibodies to the O-specific polysaccharide of their lipopolysaccharides. Clin. Infect. Dis. 15, 346–361 (1992).
Taylor, D. N. et al. Synthesis, characterization, and clinical evaluation of conjugate vaccines composed of the O-specific polysaccharides of Shigella dysenteriae type 1, Shigella flexneri type 2a, and Shigella sonnei (Plesiomonas shigelloides) bound to bacterial toxoids. Infect. Immun. 61, 3678–3687 (1993).
Cohen, D. et al. Safety and immunogenicity of investigational Shigella conjugate vaccines in Israeli volunteers. Infect. Immun. 64, 4074–4077 (1996).
Passwell, J. H. et al. Age-related efficacy of Shigella O-specific polysaccharide conjugates in 1-4-year-old Israeli children. Vaccine 28, 2231–2235 (2010).
Ihssen, J. et al. Production of glycoprotein vaccines in Escherichia coli. Microb. Cell Fact. 9, 61 (2010).
Feldman, M. F. et al. Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli. Proc. Natl Acad. Sci. USA 102, 3016–3021 (2005).
Dro, P. & Sinclair, M. GlycoVaxyn Phase I clinical study shows positive data with Shigella dysenteriae vaccine candidate [online], (2010).
Phalipon, A. et al. A synthetic carbohydrate-protein conjugate vaccine candidate against Shigella flexneri 2a infection. J. Immunol. 182, 2241–2247 (2009).
Phalipon, A. et al. Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine. J. Immunol. 176, 1686–1694 (2006).
Osorio, M., Bray, M. D. & Walker, R. I. Vaccine potential for inactivated shigellae. Vaccine 25, 1581–1592 (2007).
McKenzie, R. et al. Safety and immunogenicity of an oral, inactivated, whole-cell vaccine for Shigella sonnei: preclinical studies and a Phase I trial. Vaccine 24, 3735–3745 (2006).
Porter, C. K., Thura, N., Ranallo, R. T. & Riddle, M. S. The Shigella human challenge model. Epidemiol. Infect. 141, 223–232 (2013).
Coster, T. S. et al. Vaccination against shigellosis with attenuated Shigella flexneri 2a strain SC602. Infect. Immun. 67, 3437–3443 (1999).
Mel, D. M., Terzin, A. L. & Vuksic, L. Studies on vaccination against bacillary dysentery. 3. Effective oral immunization against Shigella flexneri 2a in a field trial. Bull. World Health Organ. 32, 647–655 (1965).
Ranallo, R. T., Barnoy, S., Thakkar, S., Urick, T. & Venkatesan, M. M. Developing live Shigella vaccines using lambda Red recombineering. FEMS Immunol. Med. Microbiol. 47, 462–469 (2006).
Venkatesan, M. M. & Ranallo, R. T. Live-attenuated Shigella vaccines. Expert Rev. Vaccines 5, 669–686 (2006).
Barnoy, S. et al. Shigella sonnei vaccine candidates WRSs2 and WRSs3 are as immunogenic as WRSS1, a clinically tested vaccine candidate, in a primate model of infection. Vaccine 29, 6371–6378 (2011).
Ranallo, R. T. et al. Virulence, inflammatory potential, and adaptive immunity induced by Shigella flexneri msbB mutants. Infect. Immun. 78, 400–412 (2010).
Katz, D. E. et al. Two studies evaluating the safety and immunogenicity of a live, attenuated Shigella flexneri 2a vaccine (SC602) and excretion of vaccine organisms in North American volunteers. Infect. Immun. 72, 923–930 (2004).
Rahman, K. M. et al. Safety, dose, immunogenicity, and transmissibility of an oral live attenuated Shigella flexneri 2a vaccine candidate (SC602) among healthy adults and school children in Matlab, Bangladesh. Vaccine 29, 1347–1354 (2011).
Gordon, J. I., Dewey, K. G., Mills, D. A. & Medzhitov, R. M. The human gut microbiota and undernutrition. Sci. Transl. Med. 4, 137ps12 (2012).
Levine, M. M. Immunogenicity and efficacy of oral vaccines in developing countries: lessons from a live cholera vaccine. BMC Biol. 8, 129 (2010).
Hanlon, P. et al. Trial of an attenuated bovine rotavirus vaccine (RIT 4237) in Gambian infants. Lancet 1, 1342–1345 (1987).
John, T. J. & Jayabal, P. Oral polio vaccination of children in the tropics. I. The poor seroconversion rates and the absence of viral interference. Am. J. Epidemiol. 96, 263–269 (1972).
Armah, G. E. et al. Efficacy of pentavalent rotavirus vaccine against severe rotavirus gastroenteritis in infants in developing countries in sub-Saharan Africa: a randomised, double-blind, placebo-controlled trial. Lancet 376, 606–614 (2010).
Balraj, V., John, T. J., Thomas, M. & Mukundan, S. Efficacy of oral poliovirus vaccine in rural communities of North Arcot District, India. Int. J. Epidemiol. 19, 711–714 (1990).
Madhi, S. A. et al. Effect of human rotavirus vaccine on severe diarrhea in African infants. N. Engl. J. Med. 362, 289–298 (2010).
Su-Arehawaratana, P. et al. Safety and immunogenicity of different immunization regimens of CVD 103-HgR live oral cholera vaccine in soldiers and civilians in Thailand. J. Infect. Dis. 165, 1042–1048 (1992).
Turbyfill, K. R., Kaminski, R. W. & Oaks, E. V. Immunogenicity and efficacy of highly purified invasin complex vaccine from Shigella flexneri 2a. Vaccine 26, 1353–1364 (2008).
Riddle, M. S. et al. Safety and immunogenicity of an intranasal Shigella flexneri 2a Invaplex 50 vaccine. Vaccine 29, 7009–7019 (2011).
Tribble, D. et al. Safety and immunogenicity of a Shigella flexneri 2a Invaplex 50 intranasal vaccine in adult volunteers. Vaccine 28, 6076–6085 (2010).
Martinez-Becerra, F. J. et al. Broadly protective Shigella vaccine based on type III secretion apparatus proteins. Infect. Immun. 80, 1222–1231 (2012).
Li, A., Zhao, C. R., Ekwall, E. & Lindberg, A. A. Serum IgG antibody responses to Shigella invasion plasmid-coded antigens detected by immunoblot. Scand. J. Infect. Dis. 26, 435–445 (1994).
Oaks, E. V., Hale, T. L. & Formal, S. B. Serum immune response to Shigella protein antigens in rhesus monkeys and humans infected with Shigella spp. Infect. Immun. 53, 57–63 (1986).
Adamus, G., Mulczyk, M., Witkowska, D. & Romanowska, E. Protection against keratoconjunctivitis shigellosa induced by immunization with outer membrane proteins of Shigella spp. Infect. Immun. 30, 321–324 (1980).
Pore, D., Mahata, N., Pal, A. & Chakrabarti, M. K. Outer membrane protein A (OmpA) of Shigella flexneri 2a, induces protective immune response in a mouse model. PLoS ONE 6, e22663 (2011).
Berlanda Scorza, F. et al. High yield production process for Shigella outer membrane particles. PLoS ONE 7, e35616 (2012).
Robin, G. et al. Characterization and quantitative analysis of serum IgG class and subclass response to Shigella sonnei and Shigella flexneri 2a lipopolysaccharide following natural Shigella infection. J. Infect. Dis. 175, 1128–1133 (1997).
Cohen, D., Green, M. S., Block, C., Rouach, T. & Ofek, I. Serum antibodies to lipopolysaccharide and natural immunity to shigellosis in an Israeli military population. J. Infect. Dis. 157, 1068–1071 (1988).
Cohen, D., Green, M. S., Block, C., Slepon, R. & Ofek, I. Prospective study of the association between serum antibodies to lipopolysaccharide O antigen and the attack rate of shigellosis. J. Clin. Microbiol. 29, 386–389 (1991).
Van De Verg, L. L., Herrington, D. A., Boslego, J., Lindberg, A. A. & Levine, M. M. Age-specific prevalence of serum antibodies to the invasion plasmid and lipopolysaccharide antigens of Shigella species in Chilean and North American populations. J. Infect. Dis. 166, 158–161 (1992).
Oberhelman, R. A. et al. Prospective study of systemic and mucosal immune responses in dysenteric patients to specific Shigella invasion plasmid antigens and lipopolysaccharides. Infect. Immun. 59, 2341–2350 (1991).
Sayem, M. A. et al. Differential host immune responses to epidemic and endemic strains of Shigella dysenteriae type I. J. Health Popul. Nutr. 29, 429–437 (2011).
Lowell, G. H. et al. Antibody-dependent cell-mediated antibacterial activity: K lymphocytes, monocytes, and granulocytes are effective against Shigella. J. Immunol. 125, 2778–2784 (1980).
Kotloff, K. L. et al. Safety, immunogenicity, and transmissibility in humans of CVD 1203, a live oral Shigella flexneri 2a vaccine candidate attenuated by deletions in aroA and virG. Infect. Immun. 64, 4542–4548 (1996).
Kotloff, K. L. et al. Evaluation of the safety, immunogenicity, and efficacy in healthy adults of four doses of live oral hybrid Escherichia coli–Shigella flexneri 2a vaccine strain EcSf2a-2. Vaccine 13, 495–502 (1995).
Hayani, K. C. et al. Concentration of milk secretory immunoglobulin A against Shigella virulence plasmid-associated antigens as a predictor of symptom status in Shigella-infected breast-fed infants. J. Pediatr. 121, 852–856 (1992).
Cleary, T. G., Winsor, D. K., Reich, D., Ruiz-Palacios, G. & Calva, J. J. Human milk immunoglobulin A antibodies to Shigella virulence determinants. Infect. Immun. 57, 1675–1679 (1989).
Cohen, D. et al. Double-blind vaccine-controlled randomised efficacy trial of an investigational Shigella sonnei conjugate vaccine in young adults. Lancet 349, 155–159 (1997).
Raqib, R. et al. Persistence of local cytokine production in shigellosis in acute and convalescent stages. Infect. Immun. 63, 289–296 (1995).
Raqib, R. et al. Delayed and reduced adaptive humoral immune responses in children with shigellosis compared with in adults. Scand. J. Immunol. 55, 414–423 (2002).
Islam, D. & Christensson, B. Disease-dependent changes in T-cell populations in patients with shigellosis. APMIS 108, 251–260 (2000).
Islam, D., Bardhan, P. K., Lindberg, A. A. & Christensson, B. Shigella infection induces cellular activation of T and B cells and distinct species-related changes in peripheral blood lymphocyte subsets during the course of the disease. Infect. Immun. 63, 2941–2949 (1995).
Islam, D., Wretlind, B., Lindberg, A. A. & Christensson, B. Changes in the peripheral blood T-cell receptor V beta repertoire in vivo and in vitro during shigellosis. Infect. Immun. 64, 1391–1399 (1996).
Manicassamy, S. & Pulendran, B. Retinoic acid-dependent regulation of immune responses by dendritic cells and macrophages. Semin. Immunol. 21, 22–27 (2009).
Pabst, O. New concepts in the generation and functions of IgA. Nat. Rev. Immunol. 12, 821–832 (2012).
Sanz, I., Wei, C., Lee, F. E. & Anolik, J. Phenotypic and functional heterogeneity of human memory B cells. Semin. Immunol. 20, 67–82 (2008).
Simon, J. K. et al. Antigen-specific B memory cell responses to lipopolysaccharide (LPS) and invasion plasmid antigen (Ipa) B elicited in volunteers vaccinated with live-attenuated Shigella flexneri 2a vaccine candidates. Vaccine 27, 565–572 (2009).
Simon, J. K. et al. Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates. Clin. Immunol. 139, 185–192 (2011).
Traggiai, E., Puzone, R. & Lanzavecchia, A. Antigen dependent and independent mechanisms that sustain serum antibody levels. Vaccine 21 (Suppl. 2), S35–S37 (2003).
Gaston, J. S. et al. Vaccination of children in low-resource countries against Shigella is unlikely to present an undue risk of reactive arthritis. Vaccine 27, 5432–5434 (2009).
Noriega, F. R., Liao, F. M., Formal, S. B., Fasano, A. & Levine, M. M. Prevalence of Shigella enterotoxin 1 among Shigella clinical isolates of diverse serotypes. J. Infect. Dis. 172, 1408–1410 (1995).
Benjelloun-Touimi, Z., Sansonetti, P. J. & Parsot, C. SepA, the major extracellular protein of Shigella flexneri: autonomous secretion and involvement in tissue invasion. Mol. Microbiol. 17, 123–135 (1995).
Wu, T., Grassel, C., Levine, M. M. & Barry, E. M. Live attenuated Shigella dysenteriae type 1 vaccine strains overexpressing shiga toxin B subunit. Infect. Immun. 79, 4912–4922 (2011).
Kotloff, K. L. et al. Phase I evaluation of delta virG Shigella sonnei live, attenuated, oral vaccine strain WRSS1 in healthy adults. Infect. Immun. 70, 2016–2021 (2002).
Barnoy, S. et al. Characterization of WRSs2 and WRSs3, new second-generation virG(icsA)-based Shigella sonnei vaccine candidates with the potential for reduced reactogenicity. Vaccine 28, 1642–1654 (2010).
Collins, T. A. et al. Safety and colonization of two novel VirG(IcsA)-based live Shigella sonnei vaccine strains in rhesus macaques (Macaca mulatta). Comp. Med. 58, 88–94 (2008).
Ranallo, R. T., Thakkar, S., Chen, Q. & Venkatesan, M. M. Immunogenicity and characterization of WRSF2G11: a second generation live attenuated Shigella flexneri 2a vaccine strain. Vaccine 25, 2269–2278 (2007).
McKenzie, R. et al. Safety and immunogenicity of WRSd1, a live attenuated Shigella dysenteriae type 1 vaccine candidate. Vaccine 26, 3291–3296 (2008).
Venkatesan, M. M. et al. Construction, characterization, and animal testing of WRSd1, a Shigella dysenteriae 1 vaccine. Infect. Immun. 70, 2950–2958 (2002).
Cohen, D. et al. Clinical trials of Shigella vaccines in Israel. Adv. Exp. Med. Biol. 397, 159–167 (1996).
Chu, C. Y. et al. Preparation, characterization, and immunogenicity of conjugates composed of the O-specific polysaccharide of Shigella dysenteriae type 1 (Shiga's bacillus) bound to tetanus toxoid. Infect. Immun. 59, 4450–4458 (1991).
Theillet, F. X. et al. Effects of backbone substitutions on the conformational behavior of Shigella flexneri O-antigens: implications for vaccine strategy. Glycobiology 21, 109–121 (2011).
Ng, S. C., Kamm, M. A., Stagg, A. J. & Knight, S. C. Intestinal dendritic cells: their role in bacterial recognition, lymphocyte homing, and intestinal inflammation. Inflamm. Bowel Dis. 16, 1787–1807 (2010).
Joffre, O. P., Segura, E., Savina, A. & Amigorena, S. Cross-presentation by dendritic cells. Nat. Rev. Immunol. 12, 557–569 (2012).