The analysis of transcripts in situ provides valuable spatial information on gene expression at tissue-level and/or subcellular resolution. Approaches involving hybridization or sequencing have been developed, but these have been limited to characterizing the expression of only a few genes simultaneously. Lee et al. now present a new method of fluorescent in situ RNA sequencing (FISSEQ) that is applicable to various sample types from cultured cells to tissue sections and whole-mount embryos. All steps — reverse transcription, amplification and fluorescence-based sequencing — are carried out across the transcriptome at the cellular location of each transcript. Such an approach provides opportunities for spatially annotated global expression analyses.
References
Lee, J. H. et al. Highly multiplexed subcellular RNA sequencing in situ. Science http://dx.doi.org/10.1126/science.1250212 (2014)
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Burgess, D. Sequencing transcriptomes in situ. Nat Rev Genet 15, 217 (2014). https://doi.org/10.1038/nrg3708
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DOI: https://doi.org/10.1038/nrg3708