Stevens et al. sought a cost-effective alternative to whole-genome bisulphite sequencing (WGBS) for a high-resolution, whole-genome characterization of DNA methylation. They devised a statistical algorithm for combining DNA methylation data from two relatively economical methods based on methylated DNA immunoprecipitation (MeDIP–seq) or methylation-sensitive restriction enzymes (MRE–seq). This method, termed methylCRF, showed comparable resolution, coverage and accuracy to WGBS at <10% of the cost.
References
Stevens, M. et al. Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods. Genome Res. 23, 1541–1553 (2013)
Rights and permissions
About this article
Cite this article
Burgess, D. An economical route to DNA methylation profiling. Nat Rev Genet 14, 676 (2013). https://doi.org/10.1038/nrg3589
Published:
Issue Date:
DOI: https://doi.org/10.1038/nrg3589
