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Chromatin

A model for nucleosome positioning

Nature Reviews Genetics volume 13, pages 674–675 (2012)Cite this article

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Hughes and colleagues generated seven strains of Saccharomyces cerevisiae that contained yeast artificial chromosomes (YACs) with large segments of foreign DNA from three yeast species (Kluyveromyces lactis, Kluyveromyces waltii and Debaryomyces hansenii). By crosslinking the chromatin with formaldehyde and then digesting the chromatin using miccrococcal nuclease, mononucelosomal DNA could be analysed by deep sequencing. The authors were then able to compare nucleosome-mapping data between the endogenous genome and the modified S. cerevisiae strains to describe the contribution of DNA sequence ('cis') and protein factors ('trans') in nucleosome positioning further. The principle of their functional evolutionary approach is as follows: features that change when a genomic region is placed in the context of S. cerevisiae are influenced by protein factors that differ between the two species, whereas features that are maintained when the foreign DNA is present in the S. cerevisiae must be due either to intrinsic DNA sequence or to conserved trans-acting regulators.

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ORIGINAL RESEARCH PAPER

  1. Hughes, A. L. et al. A functional evolutionary approach to identify determinants of nucleosome positioning: a unifying model for establishing the genome-wide pattern. Mol. Cell 9 Aug 2012 (doi:10.1016/j.molcel.2012.07.003)

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  1. Bryony Jones
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Jones, B. A model for nucleosome positioning. Nat Rev Genet 13, 674–675 (2012). https://doi.org/10.1038/nrg3331

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  • DOI: https://doi.org/10.1038/nrg3331

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