Oncogenes are frequently activated through gene amplification (that is, an increase in copy number), which can lead to their overexpression and drive neoplastic progression. In tumour cells, amplified genomic regions (so-called 'amplicons') can occur within chromosomes, or amplification gives rise to circular extrachromosomal DNA (ecDNA). Now, Turner et al. have developed a new integrative analysis pipeline that provides insights into ecDNA biology in human cancers.
DNA sequencing can identify amplicons in tumour genomes, but it cannot spatially resolve their location in the chromosomal landscape. To ensure the unbiased detection of ecDNA, the authors developed ECdetect, a semi-automated image analysis software package for cytogenetic analyses, and integrated it with whole-genome sequencing.
ECdetect quantifies ecDNA from cells in metaphase that are stained with the fluorescent DNA-binding dye DAPI (4′,6-diamidino-2-phenylindole). The software was used to quantify ecDNA in 2,572 cells from a variety of cancer types, including 72 primary cancer cell samples, 10 immortalized tumour cell lines and 8 non-cancerous control cell lines.
Notably, ecDNA was rarely seen in normal cells but was detectable in nearly half of human cancers, both in immortalized cells and primary samples, at varying frequencies depending on tumour type. Additionally, ecDNA copy number varied from cell to cell in most tumour cultures that were analysed, demonstrating the heterogeneity of cells within a tumour.
Next, the authors carried out whole-genome sequencing of 117 tumour samples and compared the resulting reads with sequences in The Cancer Genome Atlas to validate focal amplifications. Selected amplified oncogenes in tumour cells were visualized by fluorescence in situ hybridization (FISH) and analysed using ECdetect. Interestingly, all genes tested were located solely in ecDNA, or in both ecDNA and in chromosomal homogeneously staining regions (which are indicative of gene amplification). Furthermore, quantitative PCR analysis of the oncogenes MYC and EGFRvIII (EGFR variant III) showed that they were highly expressed from ecDNA in tumour cells.
Together, these data suggest that common oncogenic driver amplifications in cancer are located on, and are expressed from, ecDNA. Importantly, mathematical modelling and the experimental validation of its predictions revealed that oncogenes on ecDNA increase copy number and tumour heterogeneity more than do those resulting from chromosomal amplification. These findings strongly suggest that ecDNA plays a crucial part in driving accelerated cancer evolution.
Turner, K. M. et al. Extrachromosomal oncogene amplification drives tumour evolution and genetic heterogeneity. Nature http://dx.doi.org/10.1038/nature21356 (2017)
About this article
Cite this article
Starling, S. ECdetect hunts extrachromosomal DNA. Nat Rev Genet 18, 212 (2017). https://doi.org/10.1038/nrg.2017.13
Revisiting characteristics of oncogenic extrachromosomal DNA as mobile enhancers on neuroblastoma and glioma cancers
Cancer Cell International (2022)
Journal of Translational Medicine (2021)
Current understanding of extrachromosomal circular DNA in cancer pathogenesis and therapeutic resistance
Journal of Hematology & Oncology (2020)