Figure 1 : Diversity of RNA species detection enabled by RNA sequencing applications.

From: Translating RNA sequencing into clinical diagnostics: opportunities and challenges

Figure 1

Various RNA sequencing (RNA-seq) methodologies can be used to measure diverse, clinically relevant RNA species. Small RNA deep sequencing uses size selection to sequence various small non-coding RNAs, including microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs). Precursor RNAs can be measured using random primer amplification and oligo(dT) primers can be used to select polyadenylated transcripts. RNA-seq also allows for detection and measurement of alternative transcripts, chimeric gene fusion transcripts and viral RNA transcripts, as well as evaluation for allele-specific expression. HPV, human papillomavirus; lncRNA, long non-coding RNA; poly(A), polyadenylation; qRT-PCR, quantitative reverse transcription PCR; rRNA, ribosomal RNA; snoRNA, small nucleolar RNA; VUSs, variants of undetermined significance.