Culturing bone marrow stromal cells on 3D silk scaffolds supports their proliferation and adipogenesis, while minimizing the activation of inflammatory pathways. Therefore, differentiation of bone marrow adipocytes in 3D culture might provide a more representative model for the study of bone marrow adipose tissue than is offered by traditional 2D cell cultures.
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The authors are supported by the US National Institutes of Health (NIH) (R24 DK092759 and RO1 DK62876 to O.A.M.; T32 DK071212 to K.T.L.).
The authors declare no competing financial interests.
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Lewis, K., MacDougald, O. Bone marrow adipocytes in 3D. Nat Rev Endocrinol 14, 254–255 (2018). https://doi.org/10.1038/nrendo.2018.31