Annex, B. H. & Simons, M. Growth factor-induced therapeutic angiogenesis in the heart: protein therapy. Cardiovasc. Res. 65, 649–655 (2005).
Gasparini, G., Longo, R., Toi, M. & Ferrara, N. Angiogenic inhibitors: a new therapeutic strategy in oncology. Nature Clin. Practice Oncol. 2, 562–577 (2005).
Nakashima, M. & Reddi, A. H. The application of bone morphogenetic proteins to dental tissue engineering. Nature Biotech. 21, 1025–1032 (2003).
Bickel, U., Yoshikawa, T. & Pardridge, W. M. Delivery of peptides and proteins through the blood–brain barrier. Adv. Drug Deliv. Rev. 46, 247–279 (2001).
Morris, M. C., Depollier, J., Mery, J., Heitz, F. & Divita, G. A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nature Biotech. 19, 1173–1176 (2001).
Isner, J. M. Arterial gene transfer of naked DNA for therapeutic angiogenesis: early clinical results. Adv. Drug Deliv. Rev. 30, 185–197 (1998).
Behlke, M. A. Progress towards in vivo use of siRNAs. Mol. Ther. 13, 644–670 (2006).
Gleave, M. E. & Monia, B. P. Antisense therapy for cancer. Nature Rev. Cancer 5, 468–479 (2005).
Gerngross, T. U. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nature Biotech. 22, 1409–1414 (2004).
Roque, A. C. A., Lowe, C. R. & Taipa, M. A. Antibodies and genetically engineered related molecules: production and purification. Biotechnol. Prog. 20, 639–654 (2004).
Pavlou, A. K. & Reichert, J. M. Recombinant protein therapeutics — success rates, market trends and values to 2010. Nature Biotech. 22, 1513–1519 (2004).
Tuszynski, M. H. et al. A Phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease. Nature. Med. 11, 551–555 (2005).
Isner, J. M. Myocardial gene therapy. Nature 415, 234–239 (2002).
Mannucci, P. M. & Tuddenham, E. G. D. The hemophilias — from royal genes to gene therapy. N. Eng. J. Med. 344, 1773–1779 (2001).
Robinson, B. W. S. & Lake, R. A. Advances in malignant mesothelioma. N. Eng. J. Med. 353, 1591–1603 (2005).
Talmadge, J. E. The pharmaceutics and delivery of therapeutic polypeptides and proteins. Adv. Drug Deliv. Rev. 10, 247–299 (1993).
Krejsa, C., Rogge, M. & Sadee, W. Protein therapeutics: new applications for pharmacogenetics. Nature Rev. Drug Discov. 5, 507–521 (2006).
Ng, E. W. M. et al. Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease. Nature Rev. Drug Discov. 5, 123–132 (2006).
Pack, D. W., Hoffman, A. S., Pun, S. & Stayton, P. S. Design and development of polymers for gene delivery. Nature Rev. Drug Discov. 4, 581–593 (2005).
Schwarze, S. R., Ho, A., Vocero-Akbani, A. & Dowdy, S. F. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285, 1569–1572 (1999).
Mastrobattista, E., van der Aa, M., Hennink, W. E. & Crommelin, D. J. A. Artificial viruses: a nanotechnological approach to gene delivery. Nature Rev. Drug Discov. 5, 115–121 (2006).
Mrsny, R. J. Strategies for targeting protein therapeutics to selected tissues and cells. Expert Opin. Biol. Ther. 4, 65–73 (2004).
Theys, J. et al. Specific targeting of cytosine deaminase to solid tumors by engineered Clostridium acetobutylicum. Cancer Gene Ther. 8, 294–297 (2001).
Luo, D. & Saltzman, W. M. Synthetic DNA delivery systems. Nature Biotech. 18, 33–37 (2000).
Park, T. G., Jeong, J. H. & Kim, S. W. Current status of polymeric gene delivery systems. Adv. Drug Deliv. Rev. 58, 467–486 (2006).
Storrie, H., Mooney, D. J. Sustained delivery of plasmid DNA from polymeric scaffolds for tissue engineering. Adv. Drug Deliv. Rev. 58, 500–514 (2006).
Lutolf, M. P. & Hubbell, J. A. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nature Biotech. 23, 47–55 (2005).
Chen, R. R. & Mooney, D. J. Polymeric growth factor delivery strategies for tissue engineering. Pharm. Res. 20, 1103–1112 (2003).
Putney, S. D. & Burke, P. A. Improving protein therapeutics with sustained-release formulations. Nature Biotech. 16, 153–157 (1998).
Langer, R. Drug delivery and targeting. Nature 392, 5–10 (1998).
Steeg, P. S. Tumor metastasis: mechanistic insights and clinical challenges. Nature Med. 12, 895–904 (2006).
Mellor, H. R. et al. Optimising non-viral gene delivery in a tumour spheroid model. J. Gene Med. 8, 1160–1170 (2006).
Jain, R. K. Normalization of tumor vasculature: an emerging concept in antiangiogenic therapy. Science 307, 58–62 (2005).
Harris, S. S. & Giorgio, T. D. Convective flow increases lipoplex delivery rate to in vitro cellular monolayers. Gene Ther. 12, 512–520 (2005).
Bhadriraju, K. & Chen, C. S. Engineering cellular microenvironments to cell-based drug testing. Drug Discov. Today 7, 612–620 (2002).
Paszek, M. J. et al. Tensional homeostasis and the malignant phenotype. Cancer Cell 8, 241–254 (2005).
Griffith, L. G. & Swartz, M. A. Capturing complex 3D tissue physiology in vitro. Nature Rev. Mol. Cell Biol. 7, 211–224 (2006).
Discher, D. E., Janmey, P. & Wang, Y. L. Tissue cells feel and respond to the stiffness of their substrate. Science 310, 1139–1143 (2005).
Sakai, T., Larsen, M. & Yamada, K. M. Fibronectin requirement in branching morphogenesis. Nature 423, 876–881 (2003).
Geiger, B., Bershadsky, A., Pankov, R. & Yamada, K. M. Transmembrane extracellular matrix–cytoskeleton crosstalk. Nature Rev. Mol. Cell Biol. 2, 793–805 (2001).
Giancotti, F. G. & Ruoslahti, E. Integrin signaling. Science 285, 1028–1032 (1999).
Ingber, D. E. & Folkman, J. How does extracellular matrix control capillary morphogenesis? Cell 58, 803–805 (1989).
Kumar, V. A., Abbas, A. & Fausto, N. Robbins & Cotran Pathologic Basis of Disease 7th edn (Elsevier Saunders, Philadelphia, 2005).
Ingber, D. E. Mechanobiology and diseases of mechanotransduction. Ann. Med. 35, 564–577 (2003).
Urban J. P., Smith, S. & Fairbank J. C. Nutrition of the intervertebral disc. Spine 29, 2700–2709 (2004).
Kovacs, E. J. & Dipietro, L. A. Fibrogenic cytokines and connective-tissue production. FASEB J. 8, 854–861 (1994).
Maghazachi, A. A. & Al-Aoukaty, A. Chemokines activate natural killer cells through heterotrimeric G-proteins: implications for the treatment of AIDS and cancer. FASEB J. 12, 913–924 (1998).
Stadelmann, W. K., Digenis, A. G. & Tobin, G. R. Impediments to wound healing. Am. J. Surg. 176, S39–S47 (1998).
Vincent, T. & Mechti, N. Extracellular matrix in bone marrow can mediate drug resistance in myeloma. Leuk. Lymphoma 46, 803–811 (2005).
Taichman, R. S. Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche. Blood 105, 2631–2639 (2005).
Calvi, L. M. et al. Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 425, 841–846 (2003).
Yang, A. D. et al. Improving delivery of antineoplastic agents with anti-vascular endothelial growth factor therapy. Cancer 103, 1561–1570 (2005).
This paper reports that a synergistic improvement in the efficiency of protein therapies can be achieved by addition of supplemental growth factors.
Richardson, T. P., Peters, M. C., Ennett, A. B. & Mooney, D. J. Polymeric system for dual growth factor delivery. Nature Biotech. 19, 1029–1034 (2001).
Woodward, T. L., Xie, J. W. & Haslam, S. Z. The role of mammary stroma in modulating the proliferative response to ovarian hormones in the normal mammary gland. J. Mammary Gland Biol. Neoplasia 3, 117–131 (1998).
Thannickal, V. J. et al. Myofibroblast differentiation by transforming growth factor-β1 is dependent on cell adhesion and integrin signaling via focal adhesion kinase. J. Biol. Chem. 278, 12384–12389 (2003).
This report discusess the importance of cell adhesion in mediating the efficiency of growth factors, which stimulate cell differentiation.
Woodward, T. L., Xie, J. W., Fendrick, J. L. & Haslam, S. Z. Proliferation of mouse mammary epithelial cells in vitro: interactions among epidermal growth factor, insulin-like growth factor I, ovarian hormones, and extracellular matrix proteins. Endocrinology 141, 3578–3586 (2000).
Pearson, R. G. et al. Spatial confinement of neurite regrowth from dorsal root ganglia within nonporous microconduits. Tissue Eng. 9, 201–208 (2003).
Korff, T. & Augustin, H. G. Tensional forces in fibrillar extracellular matrices control directional capillary sprouting. J. Cell Sci. 112, 3249–3258 (1999).
Maheshwari, G., Wells, A., Griffith, L. G. & Lauffenburger, D. A. Biophysical integration of effects of epidermal growth factor and fibronectin on fibroblast migration. Biophys. J. 76, 2814–2823 (1999).
A study demonstrating that the efficiency of growth factors that stimulate cell migration is modulated by the extent of cell adhesion.
Maheshwari, G., Brown, G., Lauffenburger, D. A., Wells, A. & Griffith, L. G. Cell adhesion and motility depend on nanoscale RGD clustering. J. Cell Sci. 113, 1677–1686 (2000).
Alobaid, N. et al. Nanocomposite containing bioactive peptides promote endothelialisation by circulating progenitor cells: an in vitro evaluation. Eur. J. Vasc. Surg. 32, 76–83 (2006).
Addison, C. L. et al. The response of VEGF-stimulated endothelial cells to angiostatic molecules is substrate-dependent. BMC Cell Biol. 6, 38 (2005).
Sutton, A. B., Canfield, A. E., Schor, S. L., Grant, M. E. & Schor, A. M. The response of endothelial-cells to TGF-β-1 is dependent upon cell-shape, proliferative state and the nature of the substratum. J. Cell Sci. 99, 777–787 (1991).
Dye, J. F. et al. Distinct patterns of microvascular endothelial cell morphology are determined by extracellular matrix composition. Endothelium 11, 151–167 (2004).
Chen, C. S., Mrkisich, M., Huang, S., Whitesides, G. M. & Ingber, D. E. Geometric control of cell life and death. Science 276, 1425–1428 (1997).
Lee, K. Y. et al. Nanoscale adhesion ligand organization regulates osteoblast proliferation and differentiation. Nano Lett. 4, 1501–1506 (2004).
Kong, H. J., Polte, T., Alsberg, E. & Mooney, D. J. FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness. Proc. Natl Acad. Sci. USA 102, 4300–4305 (2005).
Ito, Y. et al. Differential control of cellular gene expression by diffusible and non-diffusible EGF. J. Biochem. 129, 733–737 (2001).
Ehrbar, M., Metters, A., Zammaretti, P., Hubbell, J. A. & Zisch, A. H. Endothelial cell proliferation and progenitor maturation by fibrin-bound VEGF variants with differential susceptibilities to local cellular activity. J. Control. Release 101, 93–109 (2005).
Chabut, D. et al. Low molecular weight fucoidan and heparin enhance the basic fibroblast growth factor-induced tube formation of endothelial cells through heparan sulfate-dependent α6 overexpression. Mol. Pharmacol. 64, 696–702 (2003).
Wijelath, E. S. et al. Novel vascular endothelial growth factor binding domains of fibronectin enhance vascular endothelial growth factor biological activity. Circ. Res. 91, 25–31 (2002).
Elicerini, B. P. & Cheresh, D. A. The role of av integrins during angiogenesis: insights into potential mechanisms of action and clinical development. J. Clin. Invest. 103, 1227–1229 (1999).
Brooks, P. C., Clark, R. A. F. & Cheresh, D. A. Requirement of vascular integrin α(v)β(3) for angiogenesis. Science 264, 569–571 (1994).
A study showing that cellular integrin expression levels modulate the efficiency of angiogenesis-promoting growth factors.
Nakatsu, M. N. et al. Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and angiopoietin-1. Microvasc. Res. 66, 102–112 (2003).
Koblizek, T. I., Weiss, C., Yancopoulos, G. D., Deutsch, U. & Risau, W. Angiopoietin-1 induces sprouting angiogenesis in vitro. Curr. Biol. 8, 529–532 (1998).
Weaver, V. M. et al. β4 Integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium. Cancer Cell 2, 205–216 (2002).
A resport on cells that are cultured in a 3D microenvironment respond to therapeutic proteins in a distinct manner compared with cells cultured on 2D substrates.
dit Faute, M. A. et al. Distinctive alterations of invasiveness, drug resistance and cell–cell organization in 3D-cultures of MCF-7, a human breast cancer cell line, and its multidrug resistant variant. Clin. Exp. Metastasis 19, 161–167 (2002).
Wang, F. et al. Reciprocal interactions between β1-integrin and epidermal growth factor receptor in three-dimensional basement membrane breast cultures: a different perspective in epithelial biology. Proc. Natl Acad. Sci. USA 95, 14821–14826 (1998).
Minchinton, A. I. & Tannock, I. F. Drug penetration in solid tumors. Nature Rev. Cancer 6, 583–592 (2006).
Herodin, F., Bourin, P., Mayol, J. F., Lataillade, J. J. & Drouet, M. Short-term injection of antiapoptotic cytokine combinations soon after lethal γ-irradiation promotes survival. Blood 101, 2609–2616 (2003).
A paper reporting that the cellular microenvironment can mediate the resistance of cells to apoptosis induced by irradiation.
Bender, J. G., Cooney, E. M., Kandel, J. J. & Yamashiro, D. J. Vascular remodeling and clinical resistance to antiangiogenic cancer therapy. Drug Resist. Updat. 7, 289–300 (2004).
Jo, N. et al. Inhibition of platelet-derived growth factor B signaling enhances the efficacy of anti-vascular enclothelial growth factor therapy in multiple models of ocular neovascularization. Am. J. Pathol. 168, 2036–2053 (2006).
Lee, J. Y. et al. Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials. J. Control. Release 78, 187–197 (2002).
Boontheekul, T. & Mooney, D. J. Protein-based signaling systems in tissue engineering. Curr. Opin. Biotechnol. 14, 559–565 (2003).
Schmoekel, H. G. et al. Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices. Biotechnol. Bioeng. 89, 253–262 (2005).
Zisch, A. H. et al. Cell-demanded release of VEGF from synthetic, biointeractive cell-ingrowth matrices for vascularized tissue growth. FASEB J. 17, 2260–2262 (2003).
Lutolf, M. R. et al. Repair of bone defects using synthetic mimetics of collagenous extracellular matrices. Nature Biotech. 21, 513–518 (2003).
A study demonstrating that growth-factor release from synthetic ECM can be regulated by cell-mediated degradation of the ECM.
Murphy, W. L., Peters, M. C., Kohn, D. H. & Mooney, D. J. Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering. Biomaterials 21, 2521–2527 (2000).
Leach, J. K., Kaigler, D., Wang, Z., Krebsbach, P. H. & Mooney, D. J. Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. Biomaterials 27, 3249–3255 (2006).
Gwak, S. J. et al. Synergistic effect of keratinocyte transplantation and epidermal growth factor delivery on epidermal regeneration. Cell Transplant. 14, 809–817 (2005).
Davis, M. E. et al. Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction. Proc. Natl Acad. Sci. USA 103, 8155–8160 (2006).
Park, M. S., Kim, S. S., Cho, S. W., Choi, C. Y. & Kim, B. S. Enhancement of the osteogenic efficacy of osteoblast transplantation by the sustained delivery of basic fibroblast growth factor. J. Biomed. Mater. Res. 79, 353–359 (2006).
Simmons, C. A., Alsberg, E., Hsiong, S., Kim, W. J. & Mooney, D. J. Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells. Bone 35, 562–569 (2004).
Um, S. H. et al. Enzyme-catalysed assembly of DNA hydrogel. Nature Mater. 5, 797–801 (2006).
Gates, B. D. et al. New approaches to nanofabrication: molding, printing, and other techniques. Chem. Rev. 105, 1171–1196 (2005).
Sia, S. K. & Whitesides, G. M. Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies. Electrophoresis 24, 3563–3576 (2003).
Hanania, E. G. et al. Recent advances in the application of gene-therapy to human disease. Am. J. Med. 99, 537–552 (1995).
Tuszynski, M. H. & Blesch, A. Nerve growth factor: from animal models of cholinergic neuronal degeneration to gene therapy in Alzheimer's disease. Prog. Brain Res. 146, 441–449 (2004).
Yu, M., Poeschla, E. & Wongstaal, F. Progress towards gene therapy for HIV infection. Gene Ther. 1, 13–26 (1994).
Shi, F. S., Weber, S., Gan, J., Rakhmilevich, A. L. & Mahvi, D. M. Granulocyte-macrophage colony-stimulating factor (GM-CSF) secreted by cDNA-transfected tumor cells induces a more potent antitumor response than exogenous GM-CSF. Cancer Gene Ther. 6, 81–88 (1999).
Yamamoto, M. & Tabata, Y. Tissue engineering by modulated gene delivery. Adv. Drug Deliv. Rev. 58, 535–554 (2006).
Ikeda, Y. & Taira, K. Ligand-targeted delivery of therapeutic siRNA. Pharm. Res. 23, 1631–1640 (2006).
Kasid, U. & Dritschilo, A. RAF antisense oligonucleotide as a tumor radiosensitizer. Oncogene 22, 5876–5884 (2003).
Ledley, F. D. Nonviral gene therapy: the promise of genes as pharmaceutical products. Hum. Gene Ther. 6, 1129–1144 (1995).
Ge, Q. et al. Inhibition of influenza virus production in virus-infected mice by RNA interference. Proc. Natl Acad. Sci. USA 101, 8676–8681 (2004).
Lawrie, A. et al. Ultrasound enhances reporter gene expression after transfection of vascular cells in vitro. Circulation 99, 2617–2620 (1999).
A study demonstrating that external physical stimulation can regulate the efficiency of gene delivery.
Satkauskas, S. et al. Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis. Mol. Ther. 5, 133–140 (2002).
Escriou, V., Carriere, M., Bussone, F., Wils, P. & Scherman, D. Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer. J. Gene Med. 3, 179–187 (2001).
Tseng, W. C., Haselton, F. R. & Giorgio, T. D. Mitosis enhances transgene expression of plasmid delivered by cationic liposomes. Biochim. Biophys. Acta 1445, 53–64 (1999).
Hanenberg, H. et al. Colocalization of retrovirus and target cells on specific fibronectin fragments increases genetic transduction of mammalian cells. Nature Med. 2, 876–882 (1996).
Dao, M. A., Hashino, K., Kato, I. & Nolta, J. A. Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells. Blood 92, 4612–4621 (1998).
MacNeill, E. C. et al. Simultaneous infection with retroviruses pseudotyped with different envelope proteins bypasses viral receptor interference associated with colocalization of gp70 and target cells on fibronectin CH-296. J. Virol. 73, 3960–3967 (1999).
Goerner, M. et al. The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs. Blood 94, 2287–2292 (1999).
Kong, H. J., Hsiong, S. & Mooney, D. J. Nanoscale cell adhesion ligands presentation regulates non-viral gene delivery and expression. Nano Lett. 7, 161–166 (2007).
Keselowsky, B. G., Collard, D. M. & Garcia, A. J. Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion. J. Biomed. Mater. Res. A. 66, 247–259 (2003).
Shen, H., Tan, J. & Saltzman, W. M. Surface-mediated gene transfer from nanocomposites of controlled texture. Nature Mater. 3, 569–574 (2004).
Kong, H. J. et al. Non-viral gene delivery regulated by stiffness of cell adhesion substrates. Nature Mater. 4, 460–464 (2005).
A report demonstrating that the physical properties of a cell-adhesion matrix regulate the efficiency of non-viral gene uptake and expression.
Hosseinkhani, H. et al. Combination of 3D tissue engineered scaffold and non-viral gene carrier enhance in vitro DNA expression of mesenchymal stem cells. Biomaterials 27, 4269–4278 (2006).
Tidball, J. G. Mechanical signal transduction in skeletal muscle growth and adaptation. J. Appl. Physiol. 98, 1900–1908 (2005).
Huang, H. D., Kamm, R. D. & Lee, R. T. Cell mechanics and mechanotransduction: pathways, probes, and physiology. Am. J. Physiol. 287, C1–C11 (2004).
Brown, T. D. Techniques for mechanical stimulation of cells in vitro: a review. J. Biomech. 33, 3–14 (2000).
Griese, D. P. et al. Vascular gene delivery of anticoagulants by transplantation of retrovirally-transduced endothelial progenitor cells. Cardiovasc. Res. 58, 469–477 (2003).
Grove, J. E. et al. Marrow-derived cells as vehicles for delivery of gene therapy to pulmonary epithelium. Am. J. Respir. Cell Mol. Biol. 27, 645–651 (2002).
Cheung, A. T. et al. Glucose-dependent insulin release from genetically engineered K cells. Science 290, 1959–1962 (2000).
Lu, Y. X. et al. Recombinant vascular endothelial growth factor secreted from tissue-engineered bioartificial muscles promotes localized angiogenesis. Circulation 104, 594–599 (2001).
Shansky, J., Creswick, B., Lee, P., Wang, X. & Vandenburgh, H. Paracrine release of insulin-like growth factor 1 from a bioengineered tissue stimulates skeletal muscle growth in vitro. Tissue Eng. 12, 1833–1841 (2006).
Cartier, R. & Reszka, R. Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems. Gene Ther. 9, 157–167 (2002).
Andre, F. M., Cournil-Henrionnet, C., Vernerey, D., Opolon, P. & Mir, L. M. Variability of naked DNA expression after direct local injection: the influence of the injection speed. Gene Ther. 13, 1619–1627 (2006).
Riddle, K. W. et al. Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Mol. Ther. 15, 361–368 (2006).
Plopper, G. E., McNamee, H. P., Dike, L. E., Bojanowski, K. & Ingber, D. E. Convergence of integrin and growth-factor receptor signaling pathways within the focal adhesion complex. Mol. Biol. Cell 6, 1349–1365 (1995).
Nayak, B. P., Sailaja, G. & Jabbar, A. M. Augmenting the immunogenecity of DNA vaccines: role of plasmid-encoded Flt-3 ligand. as a molecular adjuvant in genetic vaccination. Virology 348, 277–288 (2006).
Li, J. M., Fan, L. M., Shah, A. & Brooks, G. Targeting αvβ3 and α5β1 for gene delivery to proliferating VSMCs: synergistic effect of TGF-β1. Am. J. Physiol. 285, H1123–H1131 (2003).
Shea, L. D., Smiley, E., Bonadio, J. & Mooney, D. J. DNA delivery from polymer matrices for tissue engineering. Nature Biotech. 17, 551–554 (1999).
Bonadio, J., Smiley, E., Patil, P. & Goldstein, S. Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nature Med. 5, 753–759 (1999).
This paper suggests that tissue regeneration can be promoted by the sustained release of plasmid DNA from a synthetic matrix to inwardly migrating host cells.
Huang, Y. C., Kaigler, D., Rice, K. G., Krebsbach, P. H. & Mooney, D. J. Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal cell-driven bone regeneration. J. Bone Miner. Res. 20, 848–857 (2005).
Endo, M. et al. Bone regeneration by modified gene-activated matrix: effectiveness in segmental tibial defects in rats. Tissue Eng. 12, 489–497 (2006).
Joki, T. et al. Continuous release of endostatin from microencapsulated engineered cells for tumor therapy. Nature Biotech. 19, 35–39 (2001).
Hill, E., Boontheekul, T. & Mooney, D. J. Regulating activation of transplanted cells controls tissue regeneration. Proc. Natl Acad. Sci. USA 103, 2494–2499 (2006).
Khademhosseini, A., Langer, R., Borenstein, J. & Vacanti, J. P. Microscale technologies for tissue engineering and biology. Proc. Natl Acad. Sci. USA 103, 2480–2487 (2006).
Langer, R. & Tirrell, D. A. Designing materials for biology and medicine. Nature 428, 487–492 (2004).