Supplementary Figure 6: Protocol steps: high-sensitivity Bioanalyzer profiles of post-captured final libraries. | Nature Protocols

Supplementary Figure 6: Protocol steps: high-sensitivity Bioanalyzer profiles of post-captured final libraries.

From: Target-enrichment sequencing for detailed characterization of small RNAs

Supplementary Figure 6

(a) High Sensitivity Bioanalyser profiles of post-captured libraries of 4 different biological replicates (lanes 1-4). On the left, 1 -in green- represents the lower Bioanalyser marker and 2 -in purple- shows the upper Bioanalyser marker. On the right, lane number 5 shows the negative control PCR, where no template was used. L represents the dsDNA ladder used. (b) Single nucleotide resolution scheme of post-captured libraries. N represents a given nucleotide according to the sequence of the transcriptome enriched for. X represents a given nucleotide according to the complete list of index sequences provided by ScriptSeq™ Index PCR Primers.

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