Supplementary Figure 4 : HR-MS and RP-HPLC analysis of Boc-Asp(Bzl)-AOMK.

From: Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity

Supplementary Figure 4

AOMK warhead was synthesized according to the strategy presented in the main protocol (Block B, steps 125-137), and purified via extraction. The purity of the product warhead was confirmed using analytical HPLC (UV detector, C8 column, 220nm). The molecular mass of the compound was confirmed using High Resolution Mass Spectrometer WATERS LCT premier XE with Electrospray Ionization (ESI) and Time of Flight (TOF) module.