Perspective | Published:

Quantitative proteomics: challenges and opportunities in basic and applied research

Nature Protocols volume 12, pages 12891294 (2017) | Download Citation

Abstract

In this Perspective, we discuss developments in mass-spectrometry-based proteomic technology over the past decade from the viewpoint of our laboratory. We also reflect on existing challenges and limitations, and explore the current and future roles of quantitative proteomics in molecular systems biology, clinical research and personalized medicine.

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Acknowledgements

This work was supported by the Human Frontier Science Program (grant LT000737/2016 to O.T.S.), the Swiss National Science Foundation (R.A.; grant P2EZP3_165280 to O.T.S.; grant P2EZP3_162268 to H.L.R.; Ambizione grant PZ00P3_161435 to B.C.C.), EMBO (grant ALTF_854-2015 to H.L.R.), the Swiss Initiative for Systems Biology (SystemsX.ch; to R.A.) and the European Union via ERC (grant AdG-670821 to R.A.).

Author information

Author notes

    • Hannes L Röst
    • , Ben C Collins
    •  & George Rosenberger

    These authors contributed equally to this work.

Affiliations

  1. Department of Human Genetics, University of California, Los Angeles, Los Angeles, California, USA.

    • Olga T Schubert
  2. Department of Genetics, Stanford University, Stanford, California, USA.

    • Hannes L Röst
  3. Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.

    • Ben C Collins
    • , George Rosenberger
    •  & Ruedi Aebersold
  4. Faculty of Science, University of Zurich, Zurich, Switzerland.

    • Ruedi Aebersold

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Contributions

O.T.S., H.L.R., B.C.C., G.R. and R.A. prepared the manuscript.

Competing interests

R.A. holds shares of Biognosys AG, which operates in the field covered by this Perspective.

Corresponding author

Correspondence to Ruedi Aebersold.

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DOI

https://doi.org/10.1038/nprot.2017.040

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