Supplementary Figure 4 : ITDRFCETSA confirmation of CBK derived hit compounds.

From: The cellular thermal shift assay for evaluating drug target interactions in cells

Supplementary Figure 4

An ITDRFCETSA experiment involving the human kinase p38α was performed to verify intracellular target binding of CBK200177 and CBK107148, both of which were identified in Box 4 (High throughput screening procedure for identification of stabilizers of intracellular p38α). Data are presented as the fold increase in AlphaScreen signal for each concentration of compound compared to DMSO controls. The data demonstrate dose dependent thermal stabilization of p38α for both compounds, thus confirming the results from the “High throughput screening procedure for identification of stabilizers of intracellular p38α”.