Figure 1: Plane illumination microscopy with scanned Bessel beams. | Nature Protocols

Figure 1: Plane illumination microscopy with scanned Bessel beams.

From: 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

Figure 1

(a,b) The light sheet of conventional plane illumination can be created by a sheet-like nonscanning line focus (a) or by scanning a focused Gaussian beam to produce a virtual light sheet (b). (c) Comparison of Gaussian beams at 0.5 (top) and 0.15 (middle) excitation NA and a Bessel beam with 0.5 NAOD and 0.46 NAID (bottom). The Bessel beam has a central peak slightly narrower than even the high-NA Gaussian beam, but it is at the same time as long as the low-NA Gaussian beam. However, it also has strong side lobes, which must be taken into account when it is used for plane illumination.

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