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Circular dichroism to determine binding mode and affinity of ligand–DNA interactions

Nature Protocols volume 2, pages 31663172 (2007) | Download Citation

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Abstract

Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand–DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand–DNA interactions and takes approximately 7.5 h.

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  1. James Graham Brown Cancer Center, Health Sciences Campus, University of Louisville, 529 S. Jackson Street, Louisville, Kentucky 40202, USA.

    • Nichola C Garbett
    • , Patricia A Ragazzon
    •  & Jonathan B Chaires

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Correspondence to Jonathan B Chaires.

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https://doi.org/10.1038/nprot.2007.475

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