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Tools for investigating peptide–protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach

Nature Protocols volume 2, pages 32103218 (2007) | Download Citation

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Abstract

This protocol presents the synthesis and peptide incorporation of environment-sensitive fluorescent amino acids derived from the dimethylamino-phthalimide family. The procedure uses anhydride precursors of 4-dimethylaminophthalimide (4-DMAP) or 6-dimethylaminonaphthalimide (6-DMN), whose syntheses are described in a related protocol by these authors. In this study, the corresponding fluorescent amino acids can be readily obtained in Fmoc-protected form for convenient use as building blocks in solid phase peptide synthesis (SPPS). The time required to complete the procedure depends on the size and the number of peptides targeted. Alternatively, the chromophores can be incorporated directly after SPPS via on-resin derivatization of peptides, which is an option described in a related protocol by these authors.

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Acknowledgements

This research was supported by NSF CHE-0414243 (B.I.) and the Cell Migration Consortium (GM064346). The award of a Marie Curie Fellowship to M.S. is also gratefully acknowledged.

Author information

Affiliations

  1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

    • Matthieu Sainlos
    •  & Barbara Imperiali
  2. Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

    • Barbara Imperiali

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Competing interests

The authors declare that a patent on the environment-sensitive fluorophores is pending: 'Fluorescent Probes for Biological Studies' by Imperiali et al. US Patent Application serial No. 11/106,349, filed April 13, 2005, pending.

Corresponding author

Correspondence to Barbara Imperiali.

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DOI

https://doi.org/10.1038/nprot.2007.443

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