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Changing medium and passaging cell lines

Nature Protocols volume 2, pages 2276–2284 (2007)Cite this article

Abstract

Cell lines are widely used in biomedical research. This protocol describes the methods used routinely to change the medium and passage the cells. Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth. Despite the simplicity of the methods used, each cell line has idiosyncracies. Whether working with one or several cell lines, there is no substitute for knowledge of their needs, including the range of phenotypes and growth patterns under different physical and nutrient conditions. Given the necessary care and attention, most cell lines are easy to maintain and grow.

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Acknowledgements

We thank Ian Freshney for reading and commenting on this set of protocols.

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Authors and Affiliations

  1. Department of Surgery, Prostate Cancer Research Centre, Royal Free and University College London Medical School, 67 Riding House Street, London, W1W 7EJ, UK

    John R Masters

  2. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, Hertfordshire, UK

    Glyn N Stacey

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  1. John R Masters
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  2. Glyn N Stacey
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Correspondence to John R Masters.

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The authors declare no competing financial interests.

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Masters, J., Stacey, G. Changing medium and passaging cell lines. Nat Protoc 2, 2276–2284 (2007). https://doi.org/10.1038/nprot.2007.319

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