Abstract
This protocol details a method of fabricating off-wafer, multilayered, asymmetric microparticles from the epoxy-based photosensitive polymer SU-8. Also included are methods for altering the surface properties of SU-8 and introducing functional groups for further covalent coupling reactions. These techniques may be used to create microparticles for applications in drug delivery and tissue engineering. Microparticle design, fabrication and surface modification can be completed in 2 d.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dhanaraju, M.D. et al. Biodegradation and biocompatibility of contraceptive-steroid-loaded poly (DL-lactide-co-glycolide) injectable microspheres: in vitro and in vivo study. Contraception 74, 148–156 (2006).
Jain, D., Panda, A.K. & Majumdar, D.K. Eudragit S100 entrapped insulin microspheres for oral delivery. AAPS PharmSciTech. 6, E100–E107 (2005).
Sinha, V.R. & Trehan, A. Biodegradable microspheres for parenteral delivery. Crit. Rev. Ther. Drug Carrier Syst. 22, 535–602 (2005).
Wang, J., Tabata, Y. & Morimoto, K. Aminated gelatin microspheres as a nasal delivery system for peptide drugs: evaluation of in vitro release and in vivo insulin absorption in rats. J. Control Release 113, 31–37 (2006).
Chen, R., Curran, S.J., Curran, J.M. & Hunt, J.A. The use of poly(l-lactide) and RGD modified microspheres as cell carriers in a flow intermittency bioreactor for tissue engineering cartilage. Biomaterials 27, 4453–4460 (2006).
Choi, Y.S., Park, S.N. & Suh, H. Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres. Biomaterials 26, 5855–5863 (2005).
Curran, S.J., Chen, R. Curran, J.M. & Hunt, J.A. Expansion of human chondrocytes in an intermittent stirred flow bioreactor, using modified biodegradable microspheres. Tissue Eng. 11, 1312–1322 (2005).
Kang, S.W., Jeon, O. & Kim, B.S. Poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for cartilage tissue engineering. Tissue Eng. 11, 438–447 (2005).
Mercier, N.R., Costantino, H.R., Tracy, M.A. & Bonassar, L.J. A novel injectable approach for cartilage formation in vivo using PLG microspheres. Ann. Biomed. Eng. 32, 418–429 (2004).
Mercier, N.R., Costantino, H.R., Tracy, M.A. & Bonassar, L.J. Poly(lactide-co-glycolide) microspheres as a moldable scaffold for cartilage tissue engineering. Biomaterials 26, 1945–1952 (2005).
Eiamtrakarn, S. et al. Gastrointestinal mucoadhesive patch system (GI-MAPS) for oral administration of G-CSF, a model protein. Biomaterials 23, 145–152 (2002).
He, H., Cao, X. & Lee, L.J. Design of a novel hydrogel-based intelligent system for controlled drug release. J. Control Release 95, 391–402 (2004).
Tao, S.L. & Desai, T.A. Micromachined devices: The impact of controlled geometry from cell-targeting to bioavailability. J. Control Release 109, 127–138 (2005).
Thissen, H. et al. Synthetic biodegradable microparticles for articular cartilage tissue engineering. J. Biomed. Mater. Res. A 77, 590–598 (2006).
Tao, S.L. & Desai, T.A. Microfabrication of multilayer, asymmetric, polymeric devices for drug delivery. Adv. Mater. 17, 1625–1630.
Ahmed, A., Bonner, C. & Desai, T.A. Bioadhesive microdevices with multiple reservoirs: a new platform for oral drug delivery. J. Control Release 81, 291–306 (2002).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Tao, S., Popat, K. & Desai, T. Off-wafer fabrication and surface modification of asymmetric 3D SU-8 microparticles. Nat Protoc 1, 3153–3158 (2006). https://doi.org/10.1038/nprot.2006.451
Published:
Issue Date:
DOI: https://doi.org/10.1038/nprot.2006.451
This article is cited by
-
Three-Dimensional Anisotropic Metamaterials as Triaxial Optical Inclinometers
Scientific Reports (2017)
-
Microfabrication techniques for producing freestanding multi-dimensional microstructures
Microsystem Technologies (2016)
-
A protocol for improving fabrication yield of thin SU-8 microcantilevers for use in an aptasensor
Microsystem Technologies (2015)
-
A slow cooling rate of indomethacin melt spatially confined in microcontainers increases the physical stability of the amorphous drug without influencing its biorelevant dissolution behaviour
Drug Delivery and Translational Research (2014)
-
Self-folding micropatterned polymeric containers
Biomedical Microdevices (2011)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.