Reduced glutathione and cysteine hydrochloride on crossbred bull semen

Abstract

Over the last few years, good quality freezable semen was not utilized effectively due to less knowledge about the freezability of semen. One reason is the lack of sufficient antioxidants in the seminal plasma and semen extender. The antioxidant content is reduced during the cryopreservation process and causes premature cryo-capacitation and modification of sperm membrane structure upon thawing. So the study of pre-freezing and post thaw seminal parameters like viability, motility, acrosomal integrity, lipid peroxide assay, vanguard distance traveled by sperm, mitochondrial membrane potential, velocity and motility parameters (CASA) and field fertility trial helped to assess the effectiveness of the additives. The conception rate was better in reduced glutathione than the cysteine hydrochloride treated group. The glutathione has improved the poor freezing and maintained the good freezable semen. Thus the field fertility rate was enhanced and in turn helped to prevent waste of good quality germplasm and repeat breeder syndrome in cows.