Figure 3

From: Observation of Distressed Conspecific as a Model of Emotional Trauma Generates Silent Synapses in the Prefrontal-Amygdala Pathway and Enhances Fear Learning, but Ketamine Abolishes those Effects

Figure 3

OF alters NMDAR-mediated currents. (a) Fluorescent images of an amygdala slice irradiated with a 25 μm spot (left) or a broad beam (right) of blue light. (b) Representative traces (25 per cell) of AMPAR-mediated currents elicited by paired pulses of minimal-like stimulation of dmPFC input to BLA in control (upper) and OF mice (lower). Success EPSC responses to the first pulse are shown in black, failures in gray. (c) Summary diagram of mean potencies (left) and failure rates (right) of synaptic responses in individual cells to paired stimulation in control (gray circles) and OF group (black triangles). (d) Left: representative traces (25 per cell) of NMDAR-mediated currents elicited by single pulses of minimal-like stimulation, marked as in b. Right: summary of peak amplitudes of AMPAR- and NMDAR-mediated currents evoked by minimal-like stimulation. (e) Left: averages of five traces of NMDAR-mediated currents evoked by broad beam stimulation, scaled to illustrate slower NMDAR kinetics after OF. Right: NMDAR-mediated current decay time from 90 to 30% of the peak. White and black colors represent, respectively, control and OF. Data are presented as mean±SEM. Number of cells (upper) and animals (lower) are shown. *p<0.05, **p<0.01, ***p<0.001. BLA, basolateral amygdala; dmPFC, dorsomedial prefrontal cortex; OF, observational fear.