Original Article | Published:

Elevation of Dopamine Induced by Cigarette Smoking: Novel Insights from a [11C]-(+)-PHNO PET Study in Humans

Neuropsychopharmacology volume 39, pages 415424 (2014) | Download Citation

Abstract

Positron emission tomography (PET) has convincingly provided in vivo evidence that psychoactive drugs increase dopamine (DA) levels in human brain, a feature thought critical to their reinforcing properties. Some controversy still exists concerning the role of DA in reinforcing smoking behavior and no study has explored whether smoking increases DA concentrations at the D3 receptor, speculated to have a role in nicotine’s addictive potential. Here, we used PET and [11C]-(+)-PHNO ([11C]-(+)-4-propyl-3,4,4a,5,6,10b-hexahydro-2H-naphtho[1,2-b][1,4]oxazin-9-ol) to test the hypothesis that smoking increases DA release (decreases [11C]-(+)-PHNO binding) in D2-rich striatum and D3-rich extra-striatal regions and is related to craving, withdrawal and smoking behavior. Ten participants underwent [11C]-(+)-PHNO scans after overnight abstinence and after smoking a cigarette. Motivation to smoke (smoking topography), mood, and craving were recorded. Smoking significantly decreased self-reported craving, withdrawal, and [11C]-(+)-PHNO binding in D2 and D3-rich areas (−12.0 and −15.3%, respectively). We found that motivation to smoke (puff rate) predicted magnitude of DA release in limbic striatum, and the latter was correlated with decreased craving and withdrawal symptoms. This is the first report suggesting that, in humans, DA release is increased in D3-rich areas in response to smoking. Results also support the preferential involvement of the limbic striatum in motivation to smoke, anticipation of pleasure from cigarettes and relief of withdrawal symptoms. We propose that due to the robust effect of smoking on [11C]-(+)-PHNO binding, this radiotracer represents an ideal translational tool to investigate novel therapeutic strategies targeting DA transmission.

INTRODUCTION

Most of our knowledge related to the role of dopamine (DA) in drug addiction comes from preclinical studies, but more recently positron emission tomography (PET) has allowed for the exploration of DA neurotransmission in vivo in humans. Psychostimulants drugs (such as amphetamine or cocaine) that are known to produce massive elevation in dialysate DA levels (Di Chiara and Imperato, 1988) can induce euphoria in humans, and this effect is related to decreased radiotracer (eg: [11C]-raclopride) binding interpreted as increased signaling at the D2/3 receptor (Laruelle, 2000; Laruelle et al, 1995; Volkow et al, 1999).

Unlike those psychostimulants, nicotine is known to produce relatively weak elevation in DA levels (Forget et al, 2010; Pontieri et al, 1996), which may explain why initial PET studies in animals report small, inconsistent changes in response to intravenous nicotine (Cumming et al, 2003; Marenco et al, 2004; Tsukada et al, 2002). Subsequently, in some studies smoking nicotine-containing cigarettes produced modest changes in [11C]-raclopride binding in the limbic striatum (Brody et al, 2009; Brody et al, 2006; Brody et al, 2004; Domino et al, 2012; Domino et al, 2013) or in others, changes in [11C]-raclopride binding were limited to those smokers finding the experience of nicotine/smoking pleasurable (Barrett et al, 2004; Montgomery et al, 2007). Elevations in extracellular DA induced by smoking have been associated to either craving (Brody et al, 2006; Brody et al, 2004), improved mood (Barrett et al, 2004; Brody et al, 2009; Montgomery et al, 2007) or greater puff volume (Brody et al, 2010). Therefore, the role of DA in nicotine addiction still remains unclear (refer to the study by Le Foll et al (2009a) for a review). While some authors agree that DA signaling at the D2/3 receptor appears to mediate the rewarding and motivational effects of nicotine, other have argued that DA may instead mediate aversion to acute nicotine or nicotine withdrawal (Grieder et al, 2012; Laviolette and Van Der Kooy, 2003a, 2003b). Because of this complexity, it appears that the use of smoking topography to characterize smoking behavior may yield valuable insights, as rate of nicotine administration or latency to drug-use are critically related to drug reinforcements and warrants further study.

Although the bulk of human studies have focused indistinctively on the role of DA activity at the D2 and D3 receptor, different lines of evidence, mostly from preclinical work have shown that the D3 dopamine receptor may be particularly involved in nicotine addiction (Le Foll et al, 2007). D3 antagonism reduces the influence of cues on behavior using Pavlovian conditioning procedure (Le Foll et al, 2003), nicotine-induced place preferences (Le Foll et al, 2005; Pak et al, 2006), nicotine-induced reinstatement of nicotine seeking (Andreoli et al, 2003) and cue-induced reinstatement of nicotine seeking (Khaled et al, 2010). It is now possible to investigate DA occupancy at the D3 receptor using the PET probe [11C]-(+)-PHNO (Wilson et al, 2005). Specifically, [11C]-(+)-PHNO binding (20-fold selectivity for D3 over D2) can be interpreted in a region-dependent manner, with binding in dorsal striatum (high D2/low D3 expression) more likely reflecting D2 receptor availability, and binding in hypothalamus and substantia nigra (SN) reflecting predominantly D3 availability. The ventral pallidum (VP) and globus pallidus (GP) are areas of mixed D2/D3 binding where the D3 fraction has been estimated to represent 75% and 65%, respectively (Tziortzi et al, 2011). This agonist radioligand that allows measurement of D3 receptor binding also has greater sensitivity for detecting changes in DA levels compared with the antagonist radiotracer [11C]-raclopride (Ginovart et al, 2006; Shotbolt et al, 2012). To date, no study has examined the effect of tobacco smoking on DA release in D3-rich extra-striatal areas or has investigated whether activity at this receptor accounts for motivation to smoke, craving, or mood (anxiety).

The aim of the study was to use PET/[11C]-(+)-PHNO in human smokers to investigate whether smoking increases dopamine occupancy at the D2, as well as at the D3 receptor. We hypothesized that smoking a cigarette would decreases [11C]-(+)-PHNO binding in the limbic and dorsal striatum as well as in D3-rich VP and SN. We also predicted that smoking-induced DA release would be inversely correlated with subjective craving and anxiety ratings, and positively related to smoking behavior. Individual differences in smoking behavior were investigated via smoking topography.

MATERIALS AND METHODS

Subjects

Participants between the ages of 19 and 45 were recruited using advertisements in the local newspapers and online postings. After phone screen, they came for an in-person assessment. Healthy smokers who smoked 10 cigarettes a day excluding ultra-low nicotine cigarettes, who smoked for at least 2 years and scored 4 on the Fagerström Test for Nicotine Dependence (FTND) (Heatherton et al, 1991) were enrolled. Eligible subjects did not meet criteria for substance abuse or dependence other than nicotine and caffeine at the time of the study and tested negative on broad-spectrum gas chromatography and mass spectroscopy urine drug toxicology screen. Exclusion criteria included pregnancy, claustrophobia, current psychoactive medication use, Axis I psychiatric disorders (other than nicotine dependence) as determined by the Mini International Neuropsychiatric Interview (MINI) (Sheehan et al, 1998), cardiovascular or cerebrovascular disease, history of neurological illness or head trauma, learning disabilities, and the presence of metallic implants.

Smoking Topography

Smoking topography measures the manner or intensity with which cigarettes are smoked and is postulated to be a measure of smoking reinforcement (Frederiksen et al, 1977). The Clinical Research Support System (CReSS from Borgwaldt KC, Richmond, VA), a widely used and reliable smoking topography system (Lee et al, 2003), was used to measure ad libitum smoking behavior of a single cigarette of the participant’s own brand. Reported outcome measures are number of puffs, average and total puff volume, average puff duration and inter-puff interval. Subjects attended a separate study visit to familiarize themselves with the smoking topography device.

Plasma Nicotine and Cotinine Determination

A blood sample was taken prior to [11C]-(+)-PHNO injection on both visits to determine nicotine and cotinine concentration (ng/ml) as in (Bernert et al, 1997).

Scan Visits

PET scans took place at least 24 h apart. Prior to each scan visit, the subjects were required to abstain for 12 h (overnight) from smoking and 24 h from alcohol, and limit their coffee consumption to one caffeinated drink on the day of the scan. Upon arrival at the lab, expired breath carbon monoxide (CO) (Micro III Smokerlyzer, Bedfont Instruments, Kent, England) and breath alcohol (Alco-sensor FST, Intoximeters, MO, USA) levels were measured to confirm abstinence from smoking and alcohol, respectively. CO values of 10 ppm or any other than zero breath alcohol level identified failure to meet protocol requirements and led to scan cancellation. A urine sample was also collected to rule out use of drug of abuse and pregnancy. The subjects completed the baseline battery of craving scales—21-item nicotine-specific Visual Analog Scale (VAS; 100 point scale from agree to disagree), Questionnaire of Smoking Urges (QSU) (Tiffany and Drobes, 1991), abbreviated Tobacco Craving Questionnaire (TCQ) (Heishman et al, 2003), Urge to Smoke questionnaire (Jarvik et al, 2000), Minnesota Nicotine Withdrawal Scale (MNWS) (Hughes and Hatsukami, 1986), Beck Depression Inventory (BDI) (Beck and Steer, 1987), and State-Trait Anxiety Inventory (STAI) (Smith and Lay, 1974). Subjects were not aware of the condition assigned to the first scan visit (abstinence or smoking). They were taken to the negative pressure smoking room where they either smoked one cigarette (usual brand) via the smoking topography device or idled in the room for the similar amount time (10 min). Shortly after, subjects were placed in the PET scanner in the supine position and fitted with the thermoplastic head fixation system. After an intravenous line was placed in an antecubital vein, subjects completed the ‘pre-scan’ battery of questionnaires (TCQ, QSU, VAS, UTS, MNWS, STAI), and a blood sample of 6 ml was collected for the measurement of plasma nicotine and metabolites levels. The average time between the smoking condition and radiotracer injection was 35.0±5.6 min.

PET scans were performed using Siemens-Biograph HiRez XVI (Siemens Molecular Imaging, Knoxville, TN, USA.) PET/CT camera system, which measures radioactivity in 81 brain sections with a reconstructed pixel size of 1.07 × 1.07 × 2.00 mm each with an in-plane resolution of 5 mm full-width at half maximum (FWHM). A transmission scan was acquired and the emission scan, acquired in 32-bit list mode, began after bolus injection of [11C]-(+)-PHNO (mean±SD, dose: 9.9±1.1 mCi; specific activity: 1091.8±383.4 mCi/μmol; mass: 2.3±0.7 μg; duration of the bolus injection=2 min). Emission data were reconstructed by 2D filtered back projection to yield dynamic images with 15 1-min frames and 15 5-min frames. The emission scan lasted for 90 min. Upon scan completion, subjects were asked to complete the last, ‘post-scan’, questionnaire battery (TCQ, QSU, VAS, UTS, MNWS, STAI) and were discharged.

On a separate study visit to Toronto General Hospital, all subjects underwent a proton density weighted MRI scan acquired on a 1.5 T Signa GE scanner (slice thickness, 2 mm; repetition time, >5300 ms; echo time, 13 ms; flip angle, 90°; number of excitations, 2; acquisition matrix, 256 × 256; FOV, 22 cm) for proper localization of regions of interest (ROIs).

ROI Analysis

Delineation of ROIs and time activity curve analyses for all subjects were performed using the automated image analysis software Regions of Mental Interest (ROMI) (Rusjan et al, 2006a). Functional striatal subcompartments, including sensorimotor striatum (SMST), associative striatum (AST), and limbic striatum (LST) were automatically segmented (Rusjan et al, 2006a) bilaterally as per the method described in (Martinez et al, 2003a). The globus pallidus (GP) delineation procedure used in this study was described elsewhere (Rusjan et al, 2006b); briefly, this method, which creates a probability map of gray matter voxels within the GP (in normal space), models on the procedural steps described in the study by Rusjan et al (2006a) with the exception that the segmentation algorithm (SPM2) focuses on classifying tissues in an area limited to GP, striatum and the surrounding white matter and uses the known volume of GP (3.7 cc; (Spinks et al, 2005)) as the targeted final volume of interest. Midbrain gray matter voxels within the substantia nigra (SN) region were identified using the automated procedure described in (Rusjan et al, 2006a). The boundaries of the ventral pallidum (VP) were cropped as in (Tziortzi et al, 2011). Cerebellar cortex (excluding vermis, lobules IX and X) served as reference region. [11C]-(+)-PHNO time activity curves were obtained from dynamic data, and specific binding (BPND) was estimated in each ROI using the simplified reference tissue method (SRTM) (Lammertsma and Hume, 1996). Parameter estimation was performed with PMOD (Version 2.8.5; PMOD Technologies Ltd, Zurich, Switzerland).

Voxel-Wise Parameter Estimation

Voxel-wise parameter estimations of [11C]-(+)-PHNO were generated using the basis function implementation of SRTM (Lammertsma and Hume, 1996), with the cerebellar cortex as reference region. Spatially normalized BPND maps (SPM8; Wellcome Trust Centre for Neuroimaging, London, UK) were investigated statistically to assess significant contrasts conditions using paired t-test in SPM8. Only voxels having BPND values of >0.1 were considered in the analysis. The threshold for significant clusters was set to a family-wise error (FWE) corrected at P<0.05.

Statistical Analysis

Differences in [11C]-(+)-PHNO binding across ROIs between the conditions (abstinence and smoking) were analyzed using two-way repeated-measures analyses of variance (ANOVAs) (ROI × condition) with Bonferroni-corrected post hoc comparisons (two-tailed, α=0.05). Lateralization effect was explored using repeated-measures three-way ANOVA (ROI × condition × side). The effect of the order of treatment on the differences in binding between the conditions was explored with analysis of covariance (ANCOVA) using order of visits as a covariate. Greenhouse–Geisser adjustment was applied when the hypothesis of sphericity was rejected.

Scores on the self-reported questionnaire were analyzed using two-way repeated-measures ANOVA (condition × time) with Bonferroni-corrected pairwise comparisons (two-tailed, α=0.05). Relationships between regional changes in [11C]-(+)-PHNO BPND and other outcome measures (self-reported measures, plasma levels of nicotine and metabolites, smoking topography) were analyzed using the Pearson product moment correlation coefficient. To minimize the effect of anticipation all correlations were controlled for by order of visits. We also investigated whether D3 receptor levels ([11C]-(+)-PHNO BPND in substantia nigra in abstinence condition) predicted smoking behavior (Boileau et al, 2012; Boileau et al, 2013). Smoking-induced changes in self-reported measures were estimated by comparing changes in abstinence or smoking conditions between baseline and pre-scan (ABS is the abstinence scan, NIC is the smoking scan, and BSL is the baseline measure):

(ABSBSL−ABSPRE-SCAN)−(NICBSL−NICPRE-SCAN)

RESULTS

Subject Characteristics

Out of 19 subjects enrolled, we included a total of 10 subjects in the final analysis. One subject did not complete his MRI (VP was not delineated for this case as we did not have MRI guidelines). Three participants experienced nausea from [11C]-(+)-PHNO injection and could not continue the scan, one person withdrew after the completion of the first PET scan, and others withdrew from the study prior to the first PET visit. Subject characteristics are described in Table 1. One participant tested positive for MDMA (ecstasy) on the abstinence condition (toxicology results were obtained after the completion of the scan) and later confirmed he used ecstasy 1 week prior to scan. His data were included in the analysis (no change were observed in the results while excluding this subject).

Table 1: Subject Characteristics (n=10)

Subjective Measures of Craving and Withdrawal

Two-way repeated-measures ANOVA of craving and withdrawal scores indicated a significant condition by time interaction for self-reported ‘urge to smoke for pleasurable effects’ (TCQ 2/Expectancy F(2,18)=7.7, P<0.005), ‘urge to smoke to relieve the negative affect’ (QSU/Factor 2 F(2,18)=6.9, P<0.01; TCQ 1/emotionality F(2,18)=8.3, P<0.005) and overall urge to smoke (UTS, F(2,18)=8.9, P<0.005). Specifically, the craving scores were significantly lower after smoking compared with same-day baseline level, whereas in abstinence condition the cravings did not significantly differ between the time points P<0.05; see Figure 1). Separate main effects of condition and time on ‘anticipation of positive effects of smoking’ (QSU Factor 1 F(1,9)=13.172, P<0.01 and F(2,18)=8.585, P<0.005, respectively) and ‘intention to smoke’ (TCQ 4/Purposefulness F(1,9)=10.140, P<0.05 and F(2,18)=4.622, P<0.05) were observed such that these measures were significantly lower during the smoking condition (P<0.05) and reduced from same-day baseline (P<0.05). Subjects reported less-pronounced withdrawal symptoms and anxiety under the smoking condition (main effect of condition for MNWS F(1,9)=7.136, P<0.05 and STAI F(1,9)=7.946, P<0.05); however, the interaction with time was marginally significant for withdrawal (P=0.06) and non-significant for anxiety (P=0.61, respectively). Visual Analog Scale items—‘I have a craving for a cigarette’ and ‘I feel impatient’—significantly changed over time (VAS7 F(2,18)=10.07, P<0.005; VAS10 F(2,18)=4.715, P<0.05, respectively), such that the subjects reported lower cravings and impatience after smoking a cigarette (Bonferroni-corrected, P<0.05). Baseline scores recorded during abstinence and smoking condition across all scales were not significantly different (P corrected>0.05). Selected subjective craving measures are illustrated in Figure 1.

Figure 1
Figure 1

Subjective reports at baseline, pre- and post-scan time points in abstinence (ABS) and smoking (NIC) condition: (a). Factor 2 score (Questionnaire of Smoking Urges, QSU). (b). Emotionality factor (Tobacco Craving Questionnaire, TCQ. (c). Expectancy factor (Tobacco Craving Questionnaire, TCQ). (d). Urge to Smoke (UTS). (e). Minnesota Nicotine Withdrawal Scale (MNWS). (f). State-trait anxiety inventory (STAI). Mean±S.E.M.; *condition effect, P<0.05; #pairwise Bonferroni-corrected comparison between the time points, P<0.05.

PET [11C]-(+)-PHNO BPND

Repeated-measures ANOVA of regional [11C]-(+)-PHNO binding differences between the abstinence and smoking conditions yielded a significant condition × ROI interaction (F(1.8,14.7)=4.6, P<0.05). Pairwise comparisons revealed significant decrease in [11C]-(+)-PHNO BPND in LST (mean±SD) (−12.0±8.8%; n=10, corrected P<0.005, Cohen’s d=0.86) and VP (−15.3±15.7%; n=9, P<0.05, Cohen’s d=0.38) under the smoking condition (Figure 2). These results were replicated by voxel-wise analysis (Figure 3). The reduction in binding potential was marginally significant in SMST (−4.3±6.3%, P=0.06, Cohen’s d=0.34) and did not reach statistical significance in SN (−8.5±20%, P=0.14, Cohen’s d=0.42) and AST (−4.0±7.1%; P=0.1, Cohen’s d=0.27). Adding ROI laterality as a within-subject factor indicated significant ROI × side interaction (F(2.2,17.9)=7.034, P<0.01); however, no significant effect of side alone or its interaction with the condition effect was found. The condition × ROI interaction remained significant when ANCOVA with the order of treatment as a covariate was performed. Paired Student’s t-test indicated a significant [11C]-(+)-PHNO binding change in full striatum (−6.3±5.3%, P<0.005). There was no difference in the area under the time activity curve for the cerebellum between the smoking and abstinence condition (P=0.9).

Figure 2
Figure 2

[11C](+)PHNO BPND in abstinence (empty circles) and smoking condition (full circles) in selected ROIs (n=10, except for VP, n=9). Left y axis: SN, substantia nigra; AST, associative striatum; LST, limbic striatum; SMST, sensorimotor striatum; Right y axis: VP, ventral pallidum; *P<0.05.

Figure 3
Figure 3

T-statistical overlaid average T1 MRI showing clusters of significantly reduced [11C]-+-PHNO BPND after smoking a cigarette. Greatest decreases in [11C]-+-PHNO BPND cluster in the ventral part of the striatum and in the area that corresponds to the ventral pallidum (t max=10.3; p FEW-corrected=0.03, k=736; x=−28, y=−10, z=−4). For visualization purposes the image is threshold at a P<0.05 uncorrected.

We also analyzed data after excluding the subject who tested positive for MDMA on the abstinence condition. The 9-subject sample changes in [11C]-(+)-PHNO BPND in LST (−11.4±9.0%; P<0.05) and VP (−15.9±16.5%; P<0.05) did not significantly differ from those of the 10-subject sample (unpaired Student’s t-test, P=0.88).

Correlation Analyses

Decrease in [11C]-(+)-PHNO binding in LST after smoking a cigarette was significantly associated with greater decrease in urge to smoke in anticipation of positive effects of smoking (TCQ 2/Expectancy, r=0.71; P=0.03), and marginally correlated with the urge to smoke to relieve the negative affect (QSU/Factor 2, r=0.67; P<0.05) and overall urge to smoke (UTS, r=0.67; P<0.05). Subjects with greater smoking-induced decreases in LST [11C]-(+)-PHNO BPND also consistently reported less withdrawal symptoms (MNWS, r=0.73; P=0.03) and anxiety on smoking condition (STAI, r=0.76; P=0.02). Greater number of puffs recorded during the smoking session significantly predicted lower LST [11C]-(+)-PHNO BPND (r=−0.73, P uncorrected=0.025) and was associated with the greater relief from nicotine withdrawal or negative affect with an urgent and overwhelming desire to smoke (QSU/Factor 2 r=−0.71, P<0.05) (Figure 4). In the D3-rich VP, we found the magnitude of [11C]-(+)-PHNO displacement was related to reduced smoking-induced anxiety (STAI, r=0.7; P=0.035), but not reduced craving and withdrawal.

Figure 4
Figure 4

Relationship between [11C](+)PHNO BPND change in limbic striatum (LST), rate of smoking (puff number) and urge to smoke to relieve the negative affect (QSU/Factor 2; see in text for details on calculation). Pearson correlation coefficients (uncorrected r) and partial correlations coefficients (corrected r, controlling for the order of visits) are presented; *P<0.05.

Plasma levels of nicotine and its metabolites showed no correlation with the changes in [11C]-(+)-PHNO binding, subjective cravings and smoking behavior. D3 receptor levels (estimated in SN during abstinence) did not correlate with behavioral outcome measures and was not related to smoking-induced displacement.

DISCUSSION

To our knowledge, this is the first study to assess fluctuation of DA levels in D3- and D2-rich areas in humans using [11C]-(+)-PHNO. As expected, we found that smoking significantly attenuated craving and withdrawal scores induced by overnight abstinence. We also found that smoking had a robust effect on [11C]-(+)-PHNO binding that could be detected in whole striatum, LST and D3-rich VP. Finally, we found that increased rate of smoking predicted [11C]-(+)-PHNO displacement in LST, which was related to decreased withdrawal symptoms and urge to smoke.

We were able to detect significant differences in binding potential between the two conditions. This effect was significant for the whole striatum, but more pronounced in LST and VP. The magnitude of the effect that has been exerted on [11C]-(+)-PHNO binding by smoking appears of higher magnitude (12±8.8%) than the effects previously reported using [11C]-raclopride (8±11.8%) (Brody et al, 2009). Although [11C]-raclopride is perhaps the gold standard for measuring binding at D2/3 receptors, it has been proposed that [11C]-(+)-PHNO may be superior to measure acute fluctuations in synaptic DA release (Ginovart et al, 2006; Shotbolt et al, 2012). This higher sensitivity of [11C]-(+)-PHNO over [11C]-raclopride has also been recently confirmed in a recent study comparing the impact of nicotine and amphetamine on the binding of those two radiotracers in non-human primates (Gallezot et al, 2013). The first study conducted with smoking and [11C]-raclopride PET found no overall significant displacement (Barrett et al, 2004). Subsequently, in a study that included 20 subjects, Brody et al (2004) observed a significant displacement with [11C]-raclopride that appeared to have been found in LST, but also in the caudate and putamen. The magnitude of the DA release varied between the studies reported (Brody et al, 2004, 2009, 2006), but the largest study conducted so far with a sample of 46 subjects revealed an average decrease of 8.4±1.5% that can be detected following smoking a cigarette (Brody et al, 2009). Other groups have also reported that smoking a cigarette elicits DA release in the same range (Domino et al, 2012, 2013). In contrast to the radiotracer binding change previously observed in both ventral and dorsal striatum, our results with [11C]-(+)-PHNO suggest that the ability of smoking to lead to DA release is most pronounced in LST with no significant effect in the AST and SMST.

Importantly, due to properties of the radiotracer employed, we report for the first time that smoking is able to elicit elevation of DA in the D3-rich VP and to a lesser extent in the SN where 100% of the binding is on D3 receptors. This indicates that smoking elevates DA levels in areas outside of the striatum. The fact that there is an increase of DA transmission at the level of D3 receptors give further validity to the hypothesis that blocking DA transmission at the level of D3 could be an effective strategy for nicotine addiction (Khaled et al, 2010; Le Foll et al, 2007). Further studies should explore the role of the ventral pallidum in drug addiction (Kemppainen et al, 2012) and notably the role of the D3 receptors (Le Foll et al, 2009a; Tziortzi et al, 2011) in this area.

One of the important findings of this study is the confirmation of a significant relationship between urge to smoke and elevation of DA in LST. Previous studies using [11C-]-raclopride and smoking have linked smoking-induced DA release with hedonic response to cigarette smoking (Barrett et al, 2004), while studies with nicotine nasal spray found a correlation between nicotine-induced DA release and rating of happiness (Montgomery et al, 2007). The relationship between urge to smoke and smoking-induced DA release was found in one (Brody et al, 2004), but not all (Brody et al, 2009; Brody et al, 2006) studies where this question was examined. Here, using [11C]-(+)-PHNO, we found that only LST DA release was associated with the urge to smoke, whereas no correlation were found in other brain areas studied. This suggests that further studies should carefully analyze the different subregions of striatum when exploring such associations (Martinez et al, 2003b). Previously, a relationship between displacement of [11C-]-raclopride and mood, but not anxiety, was found in a relatively large study (Brody et al, 2009). Our study, in contrast, demonstrated a robust correlation between the subjective anxiety ratings and [11C]-(+)-PHNO displacement not only in LST, but also in VP. Some previous studies have suggested that there could be an inverse correlation between severity of nicotine dependence, as assessed with the Fagerström Test for Nicotine Dependence, and [11C-]-raclopride (Montgomery et al, 2007), although we did not observe such a relationship here, perhaps due to a limited sample size.

The exploratory correlational analysis revealed a strong relationship between withdrawal and increase in DA release. This previously unreported finding is of particular interest as numerous animal studies have suggested that the dysphoric effects associated with withdrawal could be DA-mediated (Grieder et al, 2012), and this study provides evidence for such a relationship in humans. We also found that while the total puff volume correlated with the craving and not [11C]-(+)-PHNO binding, the number of puffs subjects made during the smoking condition did correlate with both DA level elevation in LST and decrease in urge to smoke. This is in agreement with previous studies (Williams et al, 2011) where rate of smoking has been shown to be related to withdrawal symptoms attenuation. Lastly, we found no correlation between the [11C]-(+)-PHNO displacement and plasma nicotine levels, in contrast with the study by Domino et al (2013), which showed an increase in venous plasma nicotine concentrations to be correlated with increased DA release in the left caudate nucleus. This could be explained by modest rise in nicotine levels (7.8±3.6 ng/ml) obtained through controlled smoking compared with smoking to ‘satiation’ paradigm where nicotine levels achieve 30 ng/ml in 30 min (Sacco et al, 2005).

Our study has some limitations. First of all, the sample size was modest, raising the question of power. While we were powered enough to observe DA release in LST (Cohen’s d=0.86), lack of significant findings in AST and marginally significant DA release in SMST could be due to small effect size (Cohen’s d=0.27 and 0.34, for AST and SMST respectively). Furthermore, it is possible that smaller smoking-induced changes in [11C]-(+)-PHNO in dorsal striatum are potentially due to a lower displacement sensitivity in areas of minimal DRD3 fraction. However, in a recent study the bindings of both [11C]-(+)-PHNO and [11C]-raclopride were affected in the same brain areas in non-human primates following nicotine and amphetamine administration (Gallezot et al, 2013). The small sample size did not allow us to explore the gender differences or additional factors, such as gene variants, that could underlie inter-individual variability (Brody et al, 2006; Domino et al, 2012). Finally, as the significance level of the exploratory correlations between the DA release, withdrawal and smoking behavior was not corrected for multiple comparisons, these finding could be due to type I error.

We also acknowledge some design-related limitations. Owing to the institution policy, subjects were unable to smoke in the scanner; hence, smoking took place in a separate area. This led to relatively long break between cigarette smoking and radiotracer injection (35 min, on average), possibly preventing us from observing correlations between the plasma nicotine concentration and striatal DA release. The time between the cigarette smoke and the end of PET scanning was around 2 hours, a period that may not have been ideal to measure elevation of dopamine induced by smoking. However, we do not believe it was an issue. Indeed, microdialysis experiments performed in non-human primates or rodents indicate that elevation of dopamine are almost at maximal level at the time-point we selected for the beginning of the scanning time, and elevation of dopamine are prolonged and can last at least 2 hours in some reports (Domino and Tsukada, 2009; Forget et al, 2009). We also did not use denicotinized cigarette as a control. Although we believe that comparing abstinence and smoking conditions is of clinical relevance, the current design could not dissociate the role of the substances other than nicotine that are present in tobacco smoke that could modulate the reinforcing effects of nicotine or the role of conditioning factors that are thought to be critically involved in the reinforcing properties of nicotine (Le Foll and Goldberg, 2009b). The fact that administration of nicotine through nicotine spray failed to produce a significant displacement of [11C]-raclopride (Montgomery et al, 2007) suggests that conditioning factors could be important. It is noteworthy that in a recent study, presentation of smoking-associated cues was not able to affect [11C]-(+)-PHNO binding while the subjects were under the influence of nicotine (Chiuccariello et al, 2013), suggesting that at early abstinence, nicotine is more potent than cues to elicit dopamine release in human smokers. The ability of smoking cues to elicit dopamine release should be explored at a longer abstinence period in future studies. The fact that denicotinized cigarettes did not affect [11C]-raclopride binding indicates that nicotine is central to smoking-induced DA release (Brody et al, 2009).

A group of methodological caveats are related to the PET imaging acquisition and analysis procedures. The injected mass of the radiotracer in our study slightly exceed the the mass limit suggested by Gallezot et al (2012), potentially leading to underestimation of dopamine occupancy in both conditions (Shotbolt et al, 2012). The injected mass, however, did not differ between the groups and did not correlate with [11C]-(+)-PHNO BPND. In addition, as the injected mass was on average higher in the abstinence vs smoking conditions (0.031±0.011 μg/kg vs 0.026±0.008 μg/kg), the injected mass could not account for the lowest binding of [11C]-(+)-PHNO observed in the smoking condition. Moreover, the study findings are limited by the SRTM assumption of no specific binding in the cerebellum. This assumption is likely to be met, as the cerebellar standard uptake values did not differ between the conditions. Finally, although the test–retest scan reliability study for [11C]-(+)-PHNO did not show significant differences in any of the ROIs (Willeit et al, 2006), data on the reliability of smoking-induced [11C]-(+)-PHNO occupancy quantification are not available yet.

In conclusion, this study demonstrated that we can detect significant differences in limbic striatum and ventral pallidum DA levels induced by smoking (compared with abstinence) using [11C]-(+)-PHNO. We found significant correlations between elevation of DA in the limbic striatum and decreases of withdrawal, urge to smoke, and anxiety ratings. Another innovative aspect of this study is the observed correlation between DA release in limbic striatum and ventral pallidum and decrease in anxiety. This study also demonstrate that there is an increase of DA transmission at the level of D3 receptors supporting the hypothesis that blocking D3 transmission could have therapeutic value for nicotine addiction. Owing to the robust and significant effects obtained with [11C]-(+)-PHNO to detect smoking-induced DA release, we propose that this radiotracer may represent a useful tool for the assessment of novel therapeutic strategies that could modulate smoking-induced DA release and to study the impact of smoking in different populations of smokers.

FUNDING AND DISCLOSURE

Dr Guranda, Dr Wilson, Dr Houle, Dr Rusjan, Dr Busto and Dr Boileau declare no potential conflict of interest. Dr Le Foll has received grant and salary support from Pfizer Inc. and is a consultant for Richter Pharmaceuticals, Lundbeck, Mylan, Ethypharm and Pfizer. Dr Wing has received funding from the Pfizer Global Research Awards in Nicotine Dependence (GRAND) and the Pfizer Investigated Initiated Research (IIR) schemes and from the Canadian Institute of Health Research (CIHR) and the Ontario Mental Health Foundation (OMHF). Dr Zawertailo reports no conflict of interest in regards to this work. Dr Zawertailo has received grant funding from the Canadian Institutes of Health Research (CIHR), the Ontario Lung Association (OLA), Cancer Care Ontario, the Canadian Tobacco Control Research Initiative (CTCRI), the Global Research Awards for Nicotine Dependence (GRAND) and Pfizer Canada, and has received travel funds from Pfizer Canada. She has served as a paid expert witness for Avanir Pharmaceuticals. Dr Selby has no competing interests to declare with respect to this manuscript. Dr Selby has received research support from Pfizer including the Grand Award, Ministry of Health and Long term Care Ontario, Canadian Institutes of Health Research, Public Health Agency of Canada, and Health Canada; and, consulting or speaker fees from AbbVie, Pfizer Canada, Mylan pharmaceuticals and NABI pharmaceuticals. He has no personal financial interests. Dr Brody has nothing to disclose. Dr Brody’s work has been funded by the National Institute on Drug Abuse (A.L.B. [R01 DA20872]), the Tobacco-Related Disease Research Program (A.L.B. [19XT-0135]), and the Department of Veterans Affairs, Office of Research and Development (CSR&D Merit Review Award I01 CX000412 (A.L.B.)). Dr George has received consulting fees from Pfizer and Novartis, and grant/contract support from Pfizer, the National Institutes of Health, the Canada Foundation for Innovation, the Canadian Institutes of Health Research and the Ontario Mental Health Foundation.

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Acknowledgements

We would like to acknowledge the support of Research Imaging Center staff. This study was supported in part by a 2010 Pfizer GRAND Award awarded to Dr Le Foll and Canadian Foundation for Innovation.

Author information

Affiliations

  1. Translational Addiction Research Laboratory, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Bernard Le Foll
    •  & Mihail Guranda
  2. Alcohol Research and Treatment Clinic, Addiction Medicine Services, Ambulatory Care and Structured Treatments, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Bernard Le Foll
  3. Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Bernard Le Foll
    • , Alan A Wilson
    • , Sylvain Houle
    • , Pablo M Rusjan
    •  & Isabelle Boileau
  4. Department of Family and Community Medicine, University of Toronto, Toronto, ON, Canada

    • Bernard Le Foll
    •  & Peter Selby
  5. Department of Pharmacology, University of Toronto, Toronto, ON, Canada

    • Bernard Le Foll
    •  & Laurie Zawertailo
  6. Department of Psychiatry, Division of Brain and Therapeutics, University of Toronto, Toronto, ON, Canada

    • Bernard Le Foll
    • , Alan A Wilson
    • , Peter Selby
    • , Tony P George
    •  & Isabelle Boileau
  7. Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada

    • Bernard Le Foll
    • , Tony P George
    •  & Isabelle Boileau
  8. Research Imaging Centre, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Alan A Wilson
    • , Sylvain Houle
    • , Pablo M Rusjan
    •  & Isabelle Boileau
  9. Biobehavioural Addictions and Concurrent Disorders Laboratory (BACDRL), Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Victoria C Wing
    •  & Tony P George
  10. Clinical Neuroscience Program, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Laurie Zawertailo
    • , Usoa Busto
    •  & Peter Selby
  11. Addiction Program, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Laurie Zawertailo
    •  & Peter Selby
  12. Department of Psychiatry and Behavioral Sciences, University of California at Los Angeles (UCLA), Los Angeles, CA, USA

    • Arthur L Brody
  13. Addiction Imaging Research Group, Centre for Addiction and Mental Health, Toronto, ON, Canada

    • Isabelle Boileau

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Correspondence to Bernard Le Foll.

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https://doi.org/10.1038/npp.2013.209

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