Figure 4

From: Effects of Conditional Central Expression of HIV-1 Tat Protein to Potentiate Cocaine-Mediated Psychostimulation and Reward Among Male Mice

Figure 4

Human immunodeficiency virus 1 (HIV-1) Tat potentiated cocaine-conditioned place preference (CPP) does not influence the rate of extinction or the magnitude of reinstatement. (a) Time course schematic of extinction and reinstatement experiment. In the first week, mice were preference tested (triangles) the days preceding and following place-conditioning with cocaine (10 mg/kg, s.c.) and saline daily for 4 days (squares). Mice were then tested for place preference twice weekly during weeks 2, 3, and 4 (circles). After extinction (3 weeks after conditioning; 5 weeks into the experiment), mice were given a priming conditioning cycle, and tested the following day again for place preference. (b) Results of extinction and reinstatement experiment. Uninduced (white bars) and Tat-induced (striped gray bars) mice demonstrated significant cocaine-CPP compared with pre-conditioning preferences. As expected, the Tat-induced mice showed a significantly potentiated response. This potentiation was still evident 2 weeks after conditioning. Three weeks after the completion of conditioning, induced and uninduced mice demonstrated extinction of CPP. Following a priming cycle of cocaine conditioning (dashed line), both sets of mice demonstrated reinstatement of a similar magnitude of place preferences (bars to right of dashed line). *Indicates significant difference from response of uninduced mice in same test session, p<0.05, repeated measures analysis of variance (ANOVA).