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Turning up the pace of ion channel screening in drug discovery

Neuropsychopharmacology volume 34, page 253 (2009) | Download Citation

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Ion channels represent an important family of integral membrane proteins involved in many diverse physiological processes and are also implicated in a number of pathological conditions in particular of the nervous, cardiovascular, and inflammatory systems. These proteins have proven to be attractive targets for drug discovery with approximately 13% of marketed drugs having their mechanism of action attributed to activity at ligand- or voltage-gated ion channels (Overington et al, 2006). Although this success is noteworthy, there is a general consensus in the field of ion channel-targeted drug discovery that progress has been significantly hampered because of the low-throughput nature of the gold standard assay for electrophysiological assessment of ion channel activity, ie, manual patch clamp electrophysiology in mammalian cell lines expressing channels of interest. Recent innovations in the development of enabling technologies supporting higher throughput and fully automated patch clamp electrophysiology (Dunlop et al, 2008; Lu and An, 2008) have provided for a reenergizing of ion channel drug discovery with unprecedented capabilities for compound screening.

Two different approaches to achieving automation of manual patch clamp electrophysiology have recently emerged taking advantage of the so-called planar array of multi-well configurations in either a plate- or chip-based format allowing for multiple parallel recordings replacing the single channel recording typical of manual patch clamp. The IonWorks platform (Schroeder et al, 2003) was the first major innovation to be introduced and although this technology did not recapitulate the tight gigaohm seal quality typical of manual recordings, a number of assays have been successfully transferred onto this platform. Most notably, it has been possible to screen small compound libraries using the IonWorks (John et al, 2007), representing perhaps the best example of how such technologies have revolutionized ion channel screening as such a feat would be unimaginable with manual recording approaches. The second series of technologies to be introduced in the form of the PatchXpress, QPatch, and Patchliner (Dunlop et al, 2008) have successfully recapitulated the gigaohm quality seals typical of manual recordings. Until recently, these systems have relied on the parallel recording of up to 16 cells, in of itself a significant increase in screening capability. A recent innovation toward unprecedented screening capacity has been introduced in the form of a 48-channel QPatch system, a major advance over the manual recording approach where one can only imagine having 48 different individuals operating manual recording set-ups.

Despite the obvious advantages associated with fully automated ion channel screening there are challenges associated with the implementation of these technologies. Not to be underestimated is the often significant time to generate a cell line compatible with each platform, and not necessarily the cell line you have been using for many years in others applications. This process together with assay optimization and validation can be lengthy and resource intensive. However, these challenges are clearly outweighed by the now unprecedented screening capability to support ion channel-targeted drug discovery, holding much promise for expediting the discovery of new ion channel-targeted drugs.

References

  1. , , , , (2008). High-throughput electrophysiology: an emerging paradigm for ion channel screening and physiology. Nat Rev Drug Discov 7: 358–368.

  2. , , , , , et al (2007). Novel 384-well population patch clamp electrophysiology assays for Ca2+-Activated K+ channels. J Biomol Screen 12: 50–60.

  3. , (2008). Impact of novel screening technologies on ion channel drug discovery. Comb Chem High Throughput Screen 11: 185–194.

  4. , , (2006). How many drug targets are there? Nat Rev Drug Discov 5: 993–996.

  5. , , , (2003). Ionworks HT: a new high-throughput electrophysiology measurement platform. J Biomol Screen 8: 50–64.

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  1. Neuroscience Discovery Research, Wyeth Research, Princeton, NJ, USA

    • John Dunlop

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Correspondence to John Dunlop.

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DOI

https://doi.org/10.1038/npp.2008.163

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The author is a full time employee of Wyeth Research