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Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition

Nature Plants volume 3, Article number: 17105 (2017) Cite this article

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An Author Correction to this article was published on 04 May 2021

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Abstract

The phytohormone auxin induces or represses growth depending on its concentration and the underlying tissue type. However, it remains unknown how auxin signalling is modulated to allow tissues transiting between repression and promotion of growth. Here, we used apical hook development as a model for growth transitions in plants. A PIN-FORMED (PIN)-dependent intercellular auxin transport module defines an auxin maximum that is causal for growth repression during the formation of the apical hook. Our data illustrate that growth transition for apical hook opening is largely independent of this PIN module, but requires the PIN-LIKES (PILS) putative auxin carriers at the endoplasmic reticulum. PILS proteins reduce nuclear auxin signalling in the apical hook, leading to the de-repression of growth and the onset of hook opening. We also show that the phytochrome (phy) B-reliant light-signalling pathway directly regulates PILS gene activity, thereby enabling light perception to repress nuclear auxin signalling and to control growth. We propose a novel mechanism, in which PILS proteins allow external signals to alter tissue sensitivity to auxin, defining differential growth rates.

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Figure 1: PILS expression during apical hook development.
Figure 2: PILS2 and PILS5 requirements for apical hook development.
Figure 3: Light-induced apical hook opening correlates with elevated PILS expression.
Figure 4: Light-induced apical hook opening is a PILS-dependent manner.
Figure 5: Phytochrome Interacting Factor 5 represses PILS expression.
Figure 6: phyB/PIF5 pathway regulates PILS-dependent growth transition during apical hook early opening.

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Acknowledgements

We are grateful to P. Zadnikova and E. Benkova for introducing us to infrared-based time lapse imaging; D. Scheuring and F. Barbez for helping us establishing our infrared imaging station; C. Fankhauser, P. Wigge, J. Friml and A. Maizel for providing published material; E. Benkova, M. Geisler, U. Hammes and J.K.-V. group members for critical reading of the manuscript; J. Thacker for help with the manuscript; and the BOKU-VIBT Imaging Centre for access and expertise. This work was supported by the Vienna Research Group (VRG) programme of the Vienna Science and Technology Fund (WWTF), the Austrian Science Fund (FWF) (Projects: P26568-B16 and P26591-B16) and the European Research Council (ERC) (Starting Grant 639478-AuxinER) (to J.K.-V.) as well as the APART fellowship of the Austrian Academy of Sciences (ÖAW) (to D.L.).

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Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190 Vienna, Austria

    Chloé Béziat, Elke Barbez, Mugurel I. Feraru, Doris Lucyshyn & Jürgen Kleine-Vehn

  2. Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Gent, Belgium

    Chloé Béziat, Elke Barbez & Jürgen Kleine-Vehn

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  1. Chloé Béziat
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Contributions

C.B. carried out most of the experiments; J.K.-V. and C.B. interpreted the results and designed experiments. E.B. cloned most constructs and contributed to the statistical analysis. M.I.F. genotyped pils loss-of-function mutant and crosses. D.L. and C.B. performed ChIP and qPCR experiments, J.K.-V. and C.B. wrote the manuscript. All authors saw and commented on the manuscript.

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Correspondence to Jürgen Kleine-Vehn.

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The authors declare no competing financial interests.

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Supplementary Figures 1–12, Supplementary Table 1. (PDF 7526 kb)

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Béziat, C., Barbez, E., Feraru, M. et al. Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition. Nature Plants 3, 17105 (2017). https://doi.org/10.1038/nplants.2017.105

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