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The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene

Nature Plants volume 1, Article number: 15053 (2015) Cite this article

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An Erratum to this article was published on 18 May 2015

Abstract

The cyanobacterial tricarboxylic acid (TCA) cycle functions in both in biosynthesis and energy generation. However, it has until recently been generally considered to be incomplete1,2 with limited flux3,4, and few attempts have been made to draw carbon from the cycle for biotechnological purposes. We demonstrated that ethylene can be sustainably and efficiently produced from the TCA cycle of the recombinant cyanobacterium Synechocystis 6803 expressing the Pseudomonas ethylene-forming enzyme (Efe)5. A new strain with a modified ribosome binding site upstream of the efe gene diverts 10% of fixed carbon to ethylene and shows increased photosynthetic activities. The highest specific ethylene production rate reached 718 ± 19 μl l–1 h–1 per A730 nm. Experimental and computational analyses based on kinetic 13C-isotope tracer and liquid chromatography coupled with mass spectrometry (LC–MS) demonstrated that the TCA metabolism is activated by the ethylene forming reaction, resulting in a predominantly cyclic architecture. The outcome significantly enhanced flux through the remodelled TCA cycle (37% of total fixed carbon) compared with a complete, but bifurcated and low-flux (13% of total fixed carbon) TCA cycle in the wild type. Global carbon flux is redirected towards the engineered ethylene pathway. The remarkable metabolic network plasticity of this cyanobacterium is manifested by the enhancement of photosynthetic activity and redistribution of carbon flux, enabling efficient ethylene production from the TCA cycle.

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Figure 1: Ethylene production in a transgenic cyanobacterium.
Figure 2: Efe expression and ethylene productivity.
Figure 3: The TCA cycle fluxes analysed by glutamate labelling.
Figure 4: Comparison of metabolism of Synechocystis WT and the ethylene-producing strain JU547.

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Acknowledgements

This work is supported by the National Renewable Energy Laboratory Director's Fellowship (W.X.), and the DOE Energy Efficiency and Renewable Energy (EERE) BioEnergy Technologies Office (J.Y., B.W.), EERE Fuel Cell Technologies Office (P.C.M.), and DOE Office of Science BER grant DE-SC0008628 (J.A.M.). We are grateful to Jamey D. Young of Vanderbilt University for providing software and technical assistance on 13C metabolic modelling, and to Maria Ghirardi, Carrie Eckert and William Michener for helpful discussion or assistance with LC–MS equipment.

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Authors and Affiliations

  1. National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, 80401, Colorado, USA

    Wei Xiong, Justin Ungerer, Bo Wang, Pin-Ching Maness & Jianping Yu

  2. School of Chemical Engineering, Purdue University, West Lafayette, 47907, Indiana, USA

    John A. Morgan

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Contributions

W.X., J.A.M., P.C.M. and J.Y. conceived the idea, and edited the manuscript. J.U. constructed the strains. B.W. analysed the Efe protein levels. W.X. designed and performed the experiments, analysed data and wrote most of the manuscript.

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Correspondence to Jianping Yu.

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Xiong, W., Morgan, J., Ungerer, J. et al. The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene. Nature Plants 1, 15053 (2015). https://doi.org/10.1038/nplants.2015.53

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