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PsbS interactions involved in the activation of energy dissipation in Arabidopsis

Nature Plants volume 2, Article number: 15225 (2016) Cite this article

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Abstract

The non-photochemical quenching of light energy as heat (NPQ) is an important photoprotective mechanism that is activated in plants when light absorption exceeds the capacity of light utilization in photosynthesis. The PsbS protein plays a central role in this process and is supposed to activate NPQ through specific, light-regulated interactions with photosystem (PS) II antenna proteins. However, NPQ-specific interaction partners of PsbS in the thylakoid membrane are still unknown. Here, we have determined the localization and protein interactions of PsbS in thylakoid membranes in the NPQ-inactive (dark) and NPQ-active (light) states. Our results corroborate a localization of PsbS in PSII supercomplexes and support the model that the light activation of NPQ is based on the monomerization of dimeric PsbS and a light-induced enhanced interaction of PsbS with Lhcb1, the major component of trimeric light-harvesting complexes in PSII.

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Figure 1: Localization of PsbS.
Figure 2: PsbS interaction partners.
Figure 3: Relative amounts of PsbS interaction partners.
Figure 4: Model for PsbS-induced conformational changes in PSII–LHCII.

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Acknowledgements

This work was financially supported by the Deutsche Forschungsgemeinschaft (GRK 1525 to P.J.). We thank M. Benecke and K. Hoffie (IPK Gatersleben) for their highly skilled technical support.

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Authors and Affiliations

  1. Plant Biochemistry, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany

    Viviana Correa-Galvis & Peter Jahns

  2. Molecular Proteomics Laboratory, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany

    Gereon Poschmann & Kai Stühler

  3. Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany

    Michael Melzer

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  1. Viviana Correa-Galvis
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Contributions

V.C.G., G.P. and P.J. designed the experiments; V.C.G. performed the experiments; G.P. and K.S. performed mass spectrometry and analysed data; M.M. performed the electron microscopy; V.C.G. and P.J. wrote the paper.

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Correspondence to Peter Jahns.

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Correa-Galvis, V., Poschmann, G., Melzer, M. et al. PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, 15225 (2016). https://doi.org/10.1038/nplants.2015.225

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