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Inositol transporters AtINT2 and AtINT4 regulate arsenic accumulation in Arabidopsis seeds

Abstract

Arsenic contamination of groundwater and soils threatens the health of tens of millions of people worldwide. Understanding the way in which arsenic is taken up by crops such as rice, which serve as a significant source of arsenic in the human diet, is therefore important. Membrane transport proteins that catalyse arsenic uptake by roots, and translocation through the xylem to shoots, have been characterized in a number of plants, including rice. The transporters responsible for loading arsenic from the xylem into the phloem and on into the seeds, however, are yet to be identified. Here, we show that transporters responsible for inositol uptake in the phloem in Arabidopsis also transport arsenic. Transformation of Saccharomyces cerevisiae with AtINT2 or AtINT4 led to increased arsenic accumulation and increased sensitivity to arsenite. Expression of AtINT2 in Xenopus laevis oocytes also induced arsenite import. Disruption of AtINT2 or AtINT4 in Arabidopsis thaliana led to a reduction in phloem, silique and seed arsenic concentrations in plants fed with arsenite through the roots, relative to wild-type plants. These plants also exhibited a large drop in silique and seed arsenic concentrations when fed with arsenite through the leaves. We conclude that in Arabidopsis, inositol transporters are responsible for arsenite loading into the phloem, the key source of arsenic in seeds.

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Figure 1: AtINT2 and AtINT4 expression elevated S. cerevisiae sensitivity to arsenite.
Figure 2: Arsenite transportation with expression of AtINT2 and AtINT4 in yeast and oocytes.
Figure 3: Arsenite uptake inhibition by Myo-inositol.
Figure 4: Kinetic properties of AtINT2 and AtINT4 for As(III).
Figure 5: Arsenic concentration in phloem exudates, xylem sap and plant tissues.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (41371458), the special fund for agro-scientific research in the public interest (201403015) to G.L.D. and Y.G.Z, and NIH grants R15 ES022800 to Z.L. and R37 GM55425 to B.P.R.

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Y.G.Z., Z.J.L., B.P.R. and N.S. designed the research. G.L.D., Y. H., S.S., J.M., J.C. and B.D. performed research and analysed data. All authors were involved in extensive discussions and wrote the manuscript.

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Correspondence to Zijuan Liu or Yong-Guan Zhu.

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The authors declare no competing financial interests.

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Duan, GL., Hu, Y., Schneider, S. et al. Inositol transporters AtINT2 and AtINT4 regulate arsenic accumulation in Arabidopsis seeds. Nature Plants 2, 15202 (2016). https://doi.org/10.1038/nplants.2015.202

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