A polarly localized transporter for efficient manganese uptake in rice

Abstract

Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

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Figure 1: Tissue- and cell-specificity of localization of MTP9 in primary and lateral roots.
Figure 2: Subcellular localization of rice MTP9.
Figure 3: Characterization of manganese transport by proteoliposomes containing purified MTP9.
Figure 4: Effect of knockout of MTP9 on the growth and yield.
Figure 5: Comparison of manganese concentration in different organs between the wild-type rice and two mtp9 mutants.
Figure 6: Time-dependent manganese uptake.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 23119515 to D.U., No. 22119002 to J.F.M.), and by The Kurata Memorial Hitachi Science and Technology Foundation (No. 1044 to D.U.). We thank Kato, S. Yasutake, D. Rikiishi, A. Morita, T. Haruna and K. Ishii for their technical assistance.

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D.U., N.Y. and J.F.M. designed the research. D.U., A.S. and N.Y. equally contributed to the research. D.U., A.S., N.Y., Y.F., Y.T., K.I., J.C., T.M., S.M., Y.M. and J.F.M. performed the experiments, analysed data and wrote the article.

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Correspondence to Jian Feng Ma.

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The authors declare no competing financial interests.

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Ueno, D., Sasaki, A., Yamaji, N. et al. A polarly localized transporter for efficient manganese uptake in rice. Nature Plants 1, 15170 (2015). https://doi.org/10.1038/nplants.2015.170

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