Zika virus (ZIKV), a mosquito-borne flavivirus, was first identified in the 1940s in Africa, emerged in the Americas in Brazil in May 2015, with evidence that supports a causal role for this virus in a range of clinical disease presentations, collectively termed Zika congenital syndrome (ZCS), including identification of the virus in the brains of children who were still-born with microcephaly. Subsequently, the virus rapidly spread through the Americas with at least 47 countries and territories reporting clinical cases. As one might expect, many research groups in government, academia, industry, and non-governmental organizations have applied their expertise to investigating ZIKV. Prior to 2015, there had been little research on ZIKV as it was not considered to be a major pathogen and there was little existing expertise on the virus. Extraordinary progress has been made over the last year with nearly 1000 publications reporting investigations on the virus and the different clinical syndromes, demonstrating not only the excellent toolbox available to scientists today but also the speed with which multi-authors/multi-institutions can work together.
Much effort has been devoted to Zika vaccine development with at least 40 entities working on developing vaccine candidates using a variety of platforms, which have been reviewed in recent papers.1–11 In addition, to help vaccine developers, the World Health Organization published a Target Product Profile (TPP) for a Zika vaccine to be used in an emergency scenario (http://www.who.int/immunization/research/meetings_workshops/WHO_Zika_vaccine_TPP.pdf?ua=1) and a website for the vaccine pipeline tracker (http://www.who.int/immunization/research/vaccine_pipeline_tracker_spreadsheet/en/).
In the last 3 months, the first papers have been published describing nonclinical studies with candidate Zika vaccines in animal models12–16 to investigate protective immunity and passive protection, including the latest published in npj Vaccines by Muthumani et al.16 These five papers describe utilization of four platforms: purified formalin inactivated virus adjuvanted with alum (PIV), recombinant envelope (rE) protein, three different plasmid DNA constructs that all encode the ZIKV prM/E genes, and two adenovirus vectors expressing ZIKV prM/E or rE genes. All platforms induced protective immunity and passive protection in mice and non-human primates (NHPs) with at least one of the regimens investigated.
Larocca et al.12 demonstrated induction of protective immunity with a single dose of their 50 μg DNA vaccine based on a Brazilian ZIKV sequence given by the IM) route in Balb/c, SJ/L, and C57BL/6 mouse models with undetectable viremia following virus challenge (either homologous Brazilian or heterologous Puerto Rico strains by the intravenous route) at 4 or 8 weeks post immunization. Protection was correlated with the induction of neutralizing antibodies, and although CD4+ and CD8+ lymphocyte responses were induced, they were not required for protection. This group also compared their DNA vaccine with a single dose of 1 μg PIV based on the Puerto Rico strain given by the IM and SC routes. The PIV given by the IM route was superior to that given by the SC route. They followed up their mouse studies with a study in rhesus macaques. Here Abbink et al.13 compared the DNA and PIV candidate vaccines with a Rhesus adenovirus serotype 52 (RhAd52) vector-based vaccine. The PIV was given as a two-dose regimen of 5 μg PIV at 0 and 4 weeks by the SC route. The DNA vaccine was also given as a two-dose 5 mg DNA regimen at 0 and 4 weeks by the IM route, while the RhAD52 vaccine was given as a single dose of 1011 virus particle dose by the IM route. The three different platforms induced protective immunity in NHPs, including neutralizing antibodies and protection from challenge with 103 p.f.u. (plaque-forming unit) of a Brazilian strain by the SC route as measured by undetectable viremia (<100 copies/ml by qRT–PCR), and absence of detection of viral RNA in tissues that were collected in some experiments. Furthermore, neutralization titers increased post challenge, suggesting the vaccine did not induce sterilizing immunity. Interestingly, while the DNA and PIV vaccines appeared comparable in the mouse models, the PIV was superior in these limited NHP studies. Also, the RhAd52 vaccine was strongly immunogenic after one dose, while the other two vaccine platforms needed two doses.
Kim et al.14 compared immunity induced in C57BL/6 mice by a two-dose (0 and 2 weeks) regimen of either 20 μg codon-optimized recombinant E protein ectodomain of a Brazilian strain fused to the T4 fibritin foldon trimerization domain (Efl) to 1011 virus particles of the same construct on an adenovirus 5 vector (Ad5.ZIKV-Efl) following intradermal (ID) and primary SC with intranasal booster administration, respectively. Both constructs induced neutralizing antibodies, although the Efl protein induced lower neutralization titers than Ad5.ZIKV-Efl. Immunization of the Ad5.ZIKV-Efl vaccine candidate, but not the Efl candidate, resulted in passive protection in 7-day-old offspring following ip challenge with 105 pfu of ZIKV African strain Dak Ar 41542.
Dowd et al.15 utilized both mouse and rhesus macaque models using a DNA vaccine based on the French Polynesia strain. These authors used a single dose of either 2, 10, or 50 μg of DNA in Balb/c and C57BL/6 mouse models, and all immunogens induced similar levels of neutralizing antibodies (note this study did not look at the cellular immune response). For the NHP studies, this group used 1 and 4 mg per animal in a two-dose regimen (0 and 4 weeks) and compared with a single-dose regimen of 1 mg per animal. The two-dose regimen was superior to the one-dose regimen in terms of induction of neutralizing antibodies. All NHPs were challenged at 8 weeks post dose-one of vaccine with 103ffu of the Puerto Rico strain by the SC route. The one-dose vaccine was not protective while 17 of 18 animals given the two-dose regimen had no detectable viremia by qRT–PCR.
The most recent report is by Muthumani et al.16 A three-dose regimen (0, 2 and 4 weeks) of a 25 μg synthetic consensus ZIKV prM/E sequence DNA vaccine was found to induce both cellular and humoral immunity in mouse models. Significantly, in addition to immunocompetent C57BL/6 mice, these authors immunized immunocompromised mice lacking interferon αβ receptors (IFNAR−/−) mice, which is a lethal challenge model as compared with the standard viremia challenge model in immunocompetent mice. IFNAR−/− mice were immunized with either a one or a two 25 μg dose regimen (0 and 2 weeks) and challenged with 106 pfu Puerto Rico strain by the SC route. Both vaccination regimens protected the mice from weight loss and death. Subsequently, rhesus macaques were immunized by the ID route with 2 mg/dose in a two-dose regimen given at 0 and 4 weeks. Again, the vaccine induced a robust cellular and humoral immune response, including neutralizing antibodies.
Passive protection studies have been used for licensed flavivirus vaccines as part of the approval process, for example, the inactivated Japanese encephalitis vaccine.17 Here animals are passively administered with antibody (monoclonal antibodies or sera from immunized animals or humans) prior to challenge with wild-type virus. In the case of the candidate Zika vaccines, the studies describe a range of neutralization titers that are required to mediate passive protection. However, these animal studies are consistent with induction of neutralizing antibodies being a potential surrogate of protection, although the protective titer is unclear.
It is important to realize that the different studies cannot be directly compared with each other, as the methodology to measure neutralizing antibodies is different between studies with a combination of focus reduction neutralization test (FRNT), plaque reduction neutralization test (PRNT), micro-neutralization (MN), and/or reporter virus particle (RVP) assays being used. In addition, there are no antibody standards available yet with which to standardize the different neutralization methods. Nonetheless, based on passive protection studies, all five studies suggest that a neutralization titer of at least 100 is likely to be associated with a protective immune response. Interestingly, if correct, this is higher than that for current licensed flavivirus vaccines. For Japanese encephalitis (formalin inactivated, live attenuated or recombinant chimeric live attenuated), and tick-borne encephalitis (formalin inactivated) vaccines a neutralization titer of 1 in 10 is considered protective, while for yellow fever (live attenuated), a neutralization titer of 1 in 10–50 is protective. So what is different about ZIKV? One possibility to consider is that ZIKV is harder to neutralize by antibodies as compared with other flaviviruses. Basic science results support this hypothesis. Structural studies suggest that ZIKV is more stable than some other mosquito-borne flaviviruses studied,18,19 although this may be a variable phenomenon, as suggested by the recent publication by Goo et al..20 Also, panels of mouse and human monoclonal antibodies recently described neutralize ZIKV poorly compared with similar antibodies raised against dengue and West Nile viruses.21–24 Thus, basic science studies appear to be consistent with applied studies with vaccine candidates. It is possible that future basic science studies will elucidate approaches to improve the ability of immunogens to stimulate antibodies with higher affinity for ZIKV epitopes critical for eliciting neutralizing antibodies.
Overall, the nonclinical studies are consistent with induction of neutralizing antibodies being a potential surrogate of protection, but this would need substantiating with clinical studies involving immunogenicity and clinical endpoint(s) for protection, as results from animals do not always translate to the situation in humans. Studies on the durability of the vaccine-induced immune response are also limited. However, it is encouraging that the candidate vaccines induce immunity that results in undetectable viremia as measured by qRT–PCR post challenge, but the very limited data suggest that, like the currently licensed flavivirus vaccines, these candidate ZIKV vaccines may not induce sterilizing immunity. Some of the vaccine candidates reported in the nonclinical papers have advanced into clinical evaluation (NCT02840487, NCT02887482, and NCT02809443) and it will be very interesting to see how the candidate vaccines perform in immunogenicity studies.
References
Dawes, B. E. et al. Research and development of Zika virus vaccines. npj Vaccines 1, 16007 (2016).
Durbin, A. P. Vaccine development for Zika virus-timelines and strategies. Semin. Reprod. Med. 34, 299–304 (2016).
Pierson, T. C. & Graham, B. S. Zika virus: immunity and vaccine development. Cell 167, 625–631 (2016).
Martins, K. A., Dye, J. M. & Bavari, S. Considerations for the development of Zika virus vaccines. Vaccine 34, 3711–3712 (2016).
Marston, H. D., Lurie, N., Borio, L. L. & Fauci, A. S. Considerations for developing a Zika virus vaccine. N. Engl. J. Med. 375, 1209–1212 (2016).
Palacios, R., Poland, G. A. & Kalil, J. Another emerging arbovirus, another emerging vaccine: targeting Zika virus. Vaccine 34, 2291–2293 (2016).
Plotkin, S. A. Zika as still another argument for a new path to vaccine development. Clin. Microbiol. Infect. 22, 294–295 (2016).
Tripp, R. A. & Ross, T. M. Development of a Zika vaccine. Expert Rev. Vaccines. 15, 1083–1085 (2016).
Thomas, S. J., L'Azou, M., Barrett, A. D. & Jackson, N. A. Fast-track Zika vaccine development—is it possible? N. Engl.J. Med. 375, 1212–1216 (2016).
Shan, C. et al. Zika virus: diagnosis, therapeutics, and vaccine. ACS Infect. Dis 2, 170–172 (2016).
Vannice, K. S. et al. Meeting Report: WHO consultation on considerations for regulatory expectations of Zika virus vaccines for use during an emergency. Vaccine, in press.
Larocca, R. A. et al. Vaccine protection against Zika virus from Brazil. Nature 536, 474–478 (2016).
Abbink, P. et al. Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys. Science 353, 1129–1132 (2016).
Dowd, K. A. et al. Rapid development of a DNA vaccine for Zika virus. Science 354, 237–240 (2016).
Kim, E. et al. Preventive vaccines for Zika virus outbreak: preliminary evaluation. EbioMedicine, Epub ahead of print: 3 October 2016.
Muthumani, K. et al. In vivo protection against ZIKV infection and pathogenesis through passive antibody. npj Vaccines http:/dx.doi.org/10.1038/NPJVACCINES.2016.21 (2016).
Van Gessel, Y. et al. Correlation of protection against JE vaccine IXIARO induced neutralizing antibody titers. Vaccine 29, 5925–5931 (2011).
Kostyuchenko, V. A. et al. Structure of the thermally stable Zika virus. Nature 533, 425–428 (2016).
Sirohi, D. et al. The 3.8 Å resolution cryo-EM structure of Zika virus. Science 352, 467–470 (2016).
Goo, L. et al. (2016) Zika virus is not uniquely stable at physiological temperatures compared to other flaviviruses. MBio. 6, 5 (2016).
Zhao, H. et al. Structural basis of zika virus-specific antibody protection. Cell 166, 1016–1027 (2016).
Stettler, K. et al. Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection. Science 353, 823–826 (2016).
Barba-Spaeth, G. et al. Structural basis of potent Zika-dengue virus antibody cross-neutralization. Nature 536, 48–53 (2016).
Dai, L. et al. Structures of the Zika virus envelope protein and its complex with a flavivirus broadly protective antibody. Cell Host Microbe 19, 696–704 (2016).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no conflict of interest.
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Barrett, A. Zika vaccine candidates progress through nonclinical development and enter clinical trials. npj Vaccines 1, 16023 (2016). https://doi.org/10.1038/npjvaccines.2016.23
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1038/npjvaccines.2016.23
This article is cited by
-
Construction of a recombinant avipoxvirus expressing the env gene of Zika virus as a novel putative preventive vaccine
Virology Journal (2021)
-
E90 subunit vaccine protects mice from Zika virus infection and microcephaly
Acta Neuropathologica Communications (2018)
-
Current status of Zika vaccine development: Zika vaccines advance into clinical evaluation
npj Vaccines (2018)
-
Protective efficacy of Zika vaccine in AG129 mouse model
Scientific Reports (2017)
-
A live-attenuated Zika virus vaccine candidate induces sterilizing immunity in mouse models
Nature Medicine (2017)