Cell viability and recovery after clinorotation. (a) Cell spreading dynamics were measured for cells after 1, 4, and 8 h of clinorotation at 75 rpm. Four hours after clinorotation was terminated, cell areas were observed to increase by a factor of 1.4–1.8 times. (b) A live/dead stain was used to analyze both cells in the control and cells exposed to clinorotation, and revealed that cell viability was preserved over time. (c) A fluorescence micrograph for live cells (green) and dead cells (red) is provided to demonstrate the data we analyzed, dotted line indicates the axis of rotation. (d) Time-lapse micrographs illustrate a round cell adopting a more elongated morphology during recovery in standard microgravity.