Human mesenchymal stem cell shape as function of time at different rotation speeds. We analyzed the shapes of hMSCs at different rotation speeds as a function of time for 1, 4, and 8 h; black=rounded cells, line pattern=semi-elongated cells, gray=elongated. Cells were stained with phalloidin (actin, red) and representative micrographs were selected for each morphological configuration. At 0 rpm, cells were rounded at the first hour and were mostly elongated at the end of our time lapse. At 30 rpm, the number of elongated cells was reduced but still present at 4 and 8 h. At 60 rpm, there were no elongated cells at 4 h and there were mostly semi-elongated cells at 8 h. At 75 rpm, the number of rounded cells increases at every time point.