EXaCT-1 mutation correlation studies. (a) EXaCT-1 performance was validated using Reference FFPE DNA (Horizon sample) covering 11 onco-relevant variants at allele frequencies ranging from 2–25% across 6 genes commonly mutated in cancer. VAF results demonstrate a good agreement (Pearson correlation=0.76) between EXaCT-1 AF results and the expected reference DNA results. (b) Concordance between EXaCT-1 and Ion Torrent AmpliSeq assay across 9 genes (26 mutations) in 22 clinical cancer samples. The results demonstrate a good agreement between both tests over a wide range of allele frequencies with a Pearson correlation values of 0.92.