Phylogenetic and growth characteristics of Vibrio PUGSK8. (a) Phylogenetic tree of Vibrio sp. PUGSK8. Maximum parsimony consensus phylogenetic tree constructed using MEGA 6.0 based on 16S rRNA gene sequence of Vibrio sp. PUGSK8 showing representatives of other related taxa. The phylogenetic analysis showed 99% to Vibrio harveyi and Vibrio campbellii. On the basis of the AHL molecule produced by the strain PUSK8, it was tentatively identified as Vibrio campbellii strain PUGSK8. (b) The effect of PHB on growth of Vibrio PUGSK8. The growth was recorded at OD600 nm. The isolate (control) entered logarithmic phase after 9 h of growth and started to decline after 17 h. The trend of growth pattern was not affected in the media supplemented with PHB (50 μg/ml).