Figure 4

From: Amyloid proteotoxicity initiates an inflammatory response blocked by cannabinoids

Figure 4

Cytokines potentiate toxicity and Aβ accumulation. MC65 cells were either uninduced (+) or induced (−) to make Aβ in the presence or absence of IL-8 (10 ng/ml), IL-1 (10 ng/ml), interferon γ (INF-γ; 10 ng/ml) or TNFα (10 ng/ml). Sixty hours later, intracellular Aβ was determined by western blotting (antibody 6E10) (a) and cell viability by the MTT assay (b) n=4; *P<0.05; **P<0.01, ***P<0.001. AA, arachidonic acid; Aβ, beta amyloid; IFN, interferon; IL, interleukin; TNF, tumor necrosis factor.